This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Frehel, C Articles by Rastogi, N Search for Related Content PubMed PubMed Citation Articles by Frehel, C Articles by Rastogi, N

 Previous Article  |  Next Article 

Infect Immun. 1987 December; 55(12): 2916-2921

Mycobacterium leprae surface components intervene in the early phagosome-lysosome fusion inhibition event.

Département de Biologie Moléculaire, Institut Pasteur, Paris, France.

ABSTRACT

Bone marrow-derived cultured macrophages were infected with Mycobacterium leprae. The bacteria were either used as freshly isolated organisms or incubated with M. leprae antiserum (1:5) for 30 min prior to phagocytosis. Immediately after inoculation (1 to 4 h) and at 1 to 8 days later, macrophages were stained for acid phosphatase activity to assess fusions between phagosomes and lysosomes. Inhibition of fusions was essentially apparent as an early event, which was partially reversed by antiserum treatment of the bacteria, suggesting a role for M. leprae immunogenic surface components in this early phenomenon. Later incubation times (1 to 8 days) did not show any considerable difference between antiserum-treated and nontreated bacteria. The formation of an electron-transparent zone around phagocytized bacteria and its role in phagosome-lysosome fusion was investigated, and a direct relationship could not be established.

This article has been cited by other articles:

• Maurin, M., Raoult, D. (2001). Use of Aminoglycosides in Treatment of Infections Due to Intracellular Bacteria. Antimicrob. Agents Chemother. 45: 2977-2986 [Full Text]  
• Rastogi, N., Goh, K. S., Berchel, M. (1999). Species-Specific Identification of Mycobacterium leprae by PCR-Restriction Fragment Length Polymorphism Analysis of the hsp65 Gene. J. Clin. Microbiol. 37: 2016-2019 [Abstract] [Full Text]  
• Sturgill-Koszycki, S, Schlesinger, P., Chakraborty, P, Haddix, P., Collins, H., Fok, A., Allen, R., Gluck, S., Heuser, J, Russell, D. (1994). Lack of acidification in Mycobacterium phagosomes produced by exclusion of the vesicular proton-ATPase. Science 263: 678-681 [Abstract]