This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Landale, E C Articles by McCabe, M M Search for Related Content PubMed PubMed Citation Articles by Landale, E C Articles by McCabe, M M

 Previous Article  |  Next Article 

Infect Immun. 1987 December; 55(12): 3011-3016

Characterization by affinity electrophoresis of an alpha-1,6-glucan-binding protein from Streptococcus sobrinus.

Department of Microbiology and Immunology, University of Miami School of Medicine, Florida 33101.

ABSTRACT

Glucan-binding protein 1 (GBP1), the most abundant glucan-binding protein isolated from culture supernatants of Streptococcus sobrinus 6715-49, has been purified by affinity chromatography on Sephadex G-50 followed by gel permeation chromatography with Bio-Gel P-10. The specificity and affinity of GBP1 for glucans were assessed by affinity electrophoresis. GBP1 did not detectably bind to glucans lacking linear arrays of alpha-1,6 linkages. The association constant for the linear alpha-1,6-glucan Dextran T2000 was 3 x 10(7) M-1. Providing small isomaltosaccharide ligands to compete with this dextran indicated that the binding site maximally accommodated isomaltosaccharides with a degree of polymerization of 8. When glucans produced by purified S. sobrinus glucosyltransferases were tested, GBP1 displayed the highest affinity for the glucan from the soluble-product, primer-independent glucosyltransferase.

This article has been cited by other articles:

• Banas, J.A., Vickerman, M.M. (2003). GLUCAN-BINDING PROTEINSOFTHE ORAL STREPTOCOCCI. Crit. Rev. Oral Biol. Med. 14: 89-99 [Abstract] [Full Text]  
• Smith, D. J., King, W. F., Godiska, R. (2001). Passive Transfer of Immunoglobulin Y Antibody to Streptococcus mutans Glucan Binding Protein B Can Confer Protection against Experimental Dental Caries. Infect. Immun. 69: 3135-3142 [Abstract] [Full Text]  
• Haas, W., Banas, J. A. (2000). Ligand-Binding Properties of the Carboxyl-Terminal Repeat Domain of Streptococcus mutans Glucan-Binding Protein A. J. Bacteriol. 182: 728-733 [Abstract] [Full Text]  
• Smith, D. J., King, W. F., Wu, C. D., Shen, B. I, Taubman, M. A. (1998). Structural and Antigenic Characteristics of Streptococcus sobrinus Glucan Binding Proteins. Infect. Immun. 66: 5565-5569 [Abstract] [Full Text]