This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Patel, A H Articles by Foster, T Search for Related Content PubMed PubMed Citation Articles by Patel, A H Articles by Foster, T

 Previous Article  |  Next Article 

Infect Immun. 1987 December; 55(12): 3103-3110

Virulence of protein A-deficient and alpha-toxin-deficient mutants of Staphylococcus aureus isolated by allele replacement.

Microbiology Department, Moyne Institute, Dublin, Ireland.

ABSTRACT

The gene coding for protein A (spa) of Staphylococcus aureus 8325-4 has been inactivated by substituting part of the spa coding sequence for a DNA fragment specifying resistance to ethidium bromide. The in vitro-constructed spa::EtBrr substitution mutation was introduced into the S. aureus chromosome by recombinational allele replacement. Southern blot hybridization showed that the in vitro-constructed mutation was present in the chromosomal spa locus. We have previously reported the inactivation of the alpha-toxin gene (hly) by allele replacement with an in vitro-constructed hly::Emr (erythromycin resistance) mutation (M. O'Reilly, J.C.S. de Azavedo, S. Kennedy, and T.J. Foster, Microb. Pathogen. 1:125-138, 1986). A double Spa- Hly- mutant was constructed by transduction. The virulence of Spa- and Hly- mutants was tested by experimental infection of mice. When subcutaneous injections were given, Hly- mutants formed a flat, darkened lesion, whereas Hly+ strains caused a raised, cream lesion. Alpha-toxin was shown to be a major factor in forming subcutaneous lesions and in causing the death of mice injected intraperitoneally. Spa- mutants were slightly less virulent than their Spa+ counterparts, which suggests that protein A is also a virulence factor of S. aureus.

This article has been cited by other articles:

• Parcina, M., Wendt, C., Goetz, F., Zawatzky, R., Zahringer, U., Heeg, K., Bekeredjian-Ding, I. (2008). Staphylococcus aureus-Induced Plasmacytoid Dendritic Cell Activation Is Based on an IgG-Mediated Memory Response. J. Immunol. 181: 3823-3833 [Abstract] [Full Text]  
• Collins, J., Buckling, A., Massey, R. C. (2008). Identification of Factors Contributing to T-Cell Toxicity of Staphylococcus aureus Clinical Isolates. J. Clin. Microbiol. 46: 2112-2114 [Abstract] [Full Text]  
• Li, D., Cheung, A. (2008). Repression of hla by rot Is Dependent on sae in Staphylococcus aureus. Infect. Immun. 76: 1068-1075 [Abstract] [Full Text]  
• Wardenburg, J. B., Schneewind, O. (2008). Vaccine protection against Staphylococcus aureus pneumonia. J. Exp. Med. 205: 287-294 [Abstract] [Full Text]  
• Wardenburg, J. B., Patel, R. J., Schneewind, O. (2007). Surface Proteins and Exotoxins Are Required for the Pathogenesis of Staphylococcus aureus Pneumonia. Infect. Immun. 75: 1040-1044 [Abstract] [Full Text]  
• Marraffini, L. A., DeDent, A. C., Schneewind, O. (2006). Sortases and the Art of Anchoring Proteins to the Envelopes of Gram-Positive Bacteria. Microbiol. Mol. Biol. Rev. 70: 192-221 [Abstract] [Full Text]  
• Burts, M. L., Williams, W. A., DeBord, K., Missiakas, D. M. (2005). EsxA and EsxB are secreted by an ESAT-6-like system that is required for the pathogenesis of Staphylococcus aureus infections. Proc. Natl. Acad. Sci. USA 102: 1169-1174 [Abstract] [Full Text]  
• Gao, J., Stewart, G. C. (2004). Regulatory Elements of the Staphylococcus aureus Protein A (Spa) Promoter. J. Bacteriol. 186: 3738-3748 [Abstract] [Full Text]  
• Park, P. W., Foster, T. J., Nishi, E., Duncan, S. J., Klagsbrun, M., Chen, Y. (2004). Activation of Syndecan-1 Ectodomain Shedding by Staphylococcus aureus {alpha}-Toxin and {beta}-Toxin. J. Biol. Chem. 279: 251-258 [Abstract] [Full Text]  
• Sifri, C. D., Begun, J., Ausubel, F. M., Calderwood, S. B. (2003). Caenorhabditis elegans as a Model Host for Staphylococcus aureus Pathogenesis. Infect. Immun. 71: 2208-2217 [Abstract] [Full Text]  
• Gemmell, C. G., Ford, C. W. (2002). Virulence factor expression by Gram-positive cocci exposed to subinhibitory concentrations of linezolid. J Antimicrob Chemother 50: 665-672 [Abstract] [Full Text]  
• Shirtliff, M. E., Mader, J. T. (2002). Acute Septic Arthritis. Clin. Microbiol. Rev. 15: 527-544 [Abstract] [Full Text]  
• Massey, R. C., Dissanayeke, S. R., Cameron, B., Ferguson, D., Foster, T. J., Peacock, S. J. (2002). Functional Blocking of Staphylococcus aureus Adhesins following Growth in Ex Vivo Media. Infect. Immun. 70: 5339-5345 [Abstract] [Full Text]  
• Bjerketorp, J., Nilsson, M., Ljungh, A., Flock, J.-I., Jacobsson, K., Frykberg, L. (2002). A novel von Willebrand factor binding protein expressed by Staphylococcus aureus. Microbiology 148: 2037-2044 [Abstract] [Full Text]  
• Cucarella, C., Tormo, M. A., Knecht, E., Amorena, B., Lasa, I., Foster, T. J., Penades, J. R. (2002). Expression of the Biofilm-Associated Protein Interferes with Host Protein Receptors of Staphylococcus aureus and Alters the Infective Process. Infect. Immun. 70: 3180-3186 [Abstract] [Full Text]  
• Mongodin, E., Bajolet, O., Cutrona, J., Bonnet, N., Dupuit, F., Puchelle, E., Bentzmann, S. d. (2002). Fibronectin-Binding Proteins of Staphylococcus aureus Are Involved in Adherence to Human Airway Epithelium. Infect. Immun. 70: 620-630 [Abstract] [Full Text]  
• Karlsson, A., Saravia-Otten, P., Tegmark, K., Morfeldt, E., Arvidson, S. (2001). Decreased Amounts of Cell Wall-Associated Protein A and Fibronectin-Binding Proteins in Staphylococcus aureus sarA Mutants due to Up-Regulation of Extracellular Proteases. Infect. Immun. 69: 4742-4748 [Abstract] [Full Text]  
• Worlitzsch, D., Kaygin, H., Steinhuber, A., Dalhoff, A., Botzenhart, K., Döring, G. (2001). Effects of Amoxicillin, Gentamicin, and Moxifloxacin on the Hemolytic Activity of Staphylococcus aureus In Vitro and In Vivo. Antimicrob. Agents Chemother. 45: 196-202 [Abstract] [Full Text]  
• Hartleib, J., Kohler, N., Dickinson, R. B., Chhatwal, G. S., Sixma, J. J., Hartford, O. M., Foster, T. J., Peters, G., Kehrel, B. E., Herrmann, M. (2000). Protein A is the von Willebrand factor binding protein on Staphylococcus aureus. Blood 96: 2149-2156 [Abstract] [Full Text]  
• Nair, S. P., Williams, R. J., Henderson, B. (2000). Advances in our understanding of the bone and joint pathology caused by Staphylococcus aureus infection. Rheumatology (Oxford) 39: 821-834 [Full Text]  
• Silverman, G. J., Cary, S. P., Dwyer, D. C., Luo, L., Wagenknecht, R., Curtiss, V. E. (2000). A B Cell Superantigen-induced Persistent "Hole" in the B-1 Repertoire. J. Exp. Med. 192: 87-98 [Abstract] [Full Text]  
• Graille, M., Stura, E. A., Corper, A. L., Sutton, B. J., Taussig, M. J., Charbonnier, J.-B., Silverman, G. J. (2000). Crystal structure of a Staphylococcus aureus protein A domain complexed with the Fab fragment of a human IgM antibody: Structural basis for recognition of B-cell receptors and superantigen activity. Proc. Natl. Acad. Sci. USA 97: 5399-5404 [Abstract] [Full Text]  
• Goerke, C., Campana, S., Bayer, M. G., Doring, G., Botzenhart, K., Wolz, C. (2000). Direct Quantitative Transcript Analysis of the agr Regulon of Staphylococcus aureus during Human Infection in Comparison to the Expression Profile In Vitro. Infect. Immun. 68: 1304-1311 [Abstract] [Full Text]  
• Chien, Y.-t., Manna, A. C., Projan, S. J., Cheung, A. L. (1999). SarA, a Global Regulator of Virulence Determinants in Staphylococcus aureus, Binds to a Conserved Motif Essential for sar-dependent Gene Regulation. J. Biol. Chem. 274: 37169-37176 [Abstract] [Full Text]  
• McElroy, M. C., Harty, H. R., Hosford, G. E., Boylan, G. M., Pittet, J.-F., Foster, T. J. (1999). Alpha-Toxin Damages the Air-Blood Barrier of the Lung in a Rat Model of Staphylococcus aureus-Induced Pneumonia. Infect. Immun. 67: 5541-5544 [Abstract] [Full Text]  
• Wann, E. R., Fehringer, A. P., Ezepchuk, Y. V., Schlievert, P. M., Bina, P., Reiser, R. F., Hook, M. M., Leung, D. Y. M. (1999). Staphylococcus aureus Isolates from Patients with Kawasaki Disease Express High Levels of Protein A. Infect. Immun. 67: 4737-4743 [Abstract] [Full Text]  
• Navarre, W. W., Schneewind, O. (1999). Surface Proteins of Gram-Positive Bacteria and Mechanisms of Their Targeting to the Cell Wall Envelope. Microbiol. Mol. Biol. Rev. 63: 174-229 [Abstract] [Full Text]  
• Silverman, G. J., Nayak, J. V., Warnatz, K., Hajjar, F. F., Cary, S., Tighe, H., Curtiss, V. E. (1998). The Dual Phases of the Response to Neonatal Exposure to a VH Family-Restricted Staphylococcal B Cell Superantigen. J. Immunol. 161: 5720-5732 [Abstract] [Full Text]  
• Ohlsen, K., Ziebuhr, W., Koller, K.-P., Hell, W., Wichelhaus, T. A., Hacker, J. (1998). Effects of Subinhibitory Concentrations of Antibiotics on Alpha-Toxin (hla) Gene Expression of Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus Isolates. Antimicrob. Agents Chemother. 42: 2817-2823 [Abstract] [Full Text]  
• Schneewind, O, Fowler, A, Faull, K. (1995). Structure of the cell wall anchor of surface proteins in Staphylococcus aureus. Science 268: 103-106 [Abstract]  
• Mazmanian, S. K., Liu, G., Jensen, E. R., Lenoy, E., Schneewind, O. (2000). From the Cover: Staphylococcus aureus sortase mutants defective in the display of surface proteins and in the pathogenesis of animal infections. Proc. Natl. Acad. Sci. USA 97: 5510-5515 [Abstract] [Full Text]