This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Carlin, N I Articles by Lindberg, A A Search for Related Content PubMed PubMed Citation Articles by Carlin, N I Articles by Lindberg, A A

 Previous Article  |  Next Article 

Infect Immun. 1987 June; 55(6): 1412-1420

Monoclonal antibodies specific for Shigella flexneri lipopolysaccharides: clones binding to type IV, V, and VI antigens, group 3,4 antigen, and an epitope common to all Shigella flexneri and Shigella dysenteriae type 1 stains.

ABSTRACT

Monoclonal antibodies reactive with Shigella flexneri O antigens were generated in both mouse and rat systems. Antibody-producing hybridomas were screened in an enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides as antigens, and the epitope specificities were determined with a panel of lipopolysaccharides and synthetic O-antigen-specific glycoconjugates as antigens. To verify the specificity seen in the enzyme-linked immunosorbent assay, the antibodies were used in agglutination against a large number of S. flexneri strains. Monoclonal antibodies with the following specificities were identified: type, antigen IV (reactive with serotype 4a and 4b bacteria); type antigen V (reactive with serotype 5a and 5b bacteria); type antigen VI (reactive with serotype 6 bacteria); group antigen 3,4(reactive with serotype 1a, 2a, 3b, 4a, 5a, and Y bacteria); and group antigen 1 (reactive with an epitope present on all S. flexneri and Shigella dysenteriae type 1 bacteria). Furthermore, a monoclonal antibody defining a new O-antigenic epitope present on some S. flexneri strains of serotypes 4a, X, and Y was characterized (4X). The monoclonal antibodies analyzed in this study define epitopes described by polyclonal antisera (type antigens IV, V, and VI), define a hitherto uncharacterized epitope (group antigen 1), and finally identify new epitopes in what has previously been considered as one epitope (group antigen 3,4 and type antigen IV). These immunochemically characterized monoclonal antibodies may have a powerful potential in studies of the importance of humoral immunity in shigellosis.

This article has been cited by other articles:

• Phalipon, A., Costachel, C., Grandjean, C., Thuizat, A., Guerreiro, C., Tanguy, M., Nato, F., Vulliez-Le Normand, B., Belot, F., Wright, K., Marcel-Peyre, V., Sansonetti, P. J., Mulard, L. A. (2006). Characterization of Functional Oligosaccharide Mimics of the Shigella flexneri Serotype 2a O-Antigen: Implications for the Development of a Chemically Defined Glycoconjugate Vaccine. J. Immunol. 176: 1686-1694 [Abstract] [Full Text]  
• Talukder, K. A., Dutta, D. K., Safa, A., Ansaruzzaman, M., Hassan, F., Alam, K., Islam, K. M. N., Carlin, N. I. A., Nair, G. B., Sack, D. A. (2001). Altering Trends in the Dominance of Shigella flexneri Serotypes and Emergence of Serologically Atypical S. flexneri Strains in Dhaka, Bangladesh. J. Clin. Microbiol. 39: 3757-3759 [Abstract] [Full Text]  
• Adams, M. M., Allison, G. E., Verma, N. K. (2001). Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296. Microbiology 147: 851-860 [Abstract] [Full Text]  
• Adhikari, P., Allison, G., Whittle, B., Verma, N. K. (1999). Serotype 1a O-Antigen Modification: Molecular Characterization of the Genes Involved and Their Novel Organization in the Shigella flexneri Chromosome. J. Bacteriol. 181: 4711-4718 [Abstract] [Full Text]  
• El-Gendy, A., El-Ghorab, N., Lane, E. M., Elyazeed, R. A., Carlin, N. I. A., Mitry, M. M., Kay, B. A., Savarino, S. J., Peruski, L. F. Jr. (1999). Identification of Shigella flexneri Subserotype 1c in Rural Egypt. J. Clin. Microbiol. 37: 873-874 [Abstract] [Full Text]