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Infect Immun. 1988 January; 56(1): 213-218

Induction of murine cytolytic T lymphocytes by Pseudomonas aeruginosa exotoxin A.

Israel Institute for Biological Research, Ness-Ziona, Israel.

ABSTRACT

Pseudomonas aeruginosa exotoxin A (PA), a potent protein synthesis inhibitor, was found to be a weak T-cell mitogen for murine splenocytes. Maximal stimulation of [3H]thymidine incorporation was obtained with 10 to 100 ng of toxin per ml following a 4-day induction. PA was also shown to be a polyclonal activator of cytolytic T lymphocytes (CTL), effective against concanavalin A-treated target cells. The effective PA dose for CTL induction was the same as that for mitogenic stimulation, only with a prolonged priming time (7 days). In contrast to other mitogens, PA could not reactivate memory CTL into secondary CTL. The stimulation of CTL by subcytotoxic doses of PA may be relevant to its modulatory effect on the immunocellular system.

This article has been cited by other articles:

• Schumann, J., Angermuller, S., Bang, R., Lohoff, M., Tiegs, G. (1998). Acute Hepatotoxicity of Pseudomonas aeruginosa Exotoxin A in Mice Depends on T Cells and TNF. J. Immunol. 161: 5745-5754 [Abstract] [Full Text]