This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chalvignac, M A Articles by Mollaret, H H Search for Related Content PubMed PubMed Citation Articles by Chalvignac, M A Articles by Mollaret, H H

 Previous Article  |  Next Article 

Infect Immun. 1988 October; 56(10): 2576-2580

In vitro expression of a 22-kilodalton Yersinia pestis polypeptide immunologically related to the 25-kilodalton plasmid-encoded protein of the three pathogenic Yersinia species.

Unité d'Ecologie Bactérienne, Institut Pasteur, Paris, France.

ABSTRACT

Antibodies raised against the 25-kilodalton (p25) plasmid-encoded polypeptide of Yersinia enterocolitica recognized the homologous protein in the three Yersinia species grown in vitro. This polypeptide was recovered from whole cells as well as from the fluid supernatant of bacteria grown at 37 degrees C in a Ca2+-deficient medium. Furthermore, a 22-kilodalton (p22) plasmid-encoded polypeptide immunologically related to p25 was found only in Y. pestis during early growth. After 30 h of culture, the Y. pestis p25 and p22 were completely degraded, whereas the intensity of the Y. enterocolitica p25 was decreased, but the protein was still detectable in the fluid supernatant. This proteolytic activity was independent of the presence of the virulence plasmid. Some disulfide bonds are probably involved in the quaternary structure of the p25 of the three pathogenic species and of the Y. pestis p22.

This article has been cited by other articles:

• FEODOROVA, V.A., DEVDARIANI, Z.L. (2001). Immunogeneity and structural organisation of some pLCR-encoded proteins of Yersinia pestis. J Med Microbiol 50: 13-22 [Abstract] [Full Text]  
• Kutyrev, V., Mehigh, R. J., Motin, V. L., Pokrovskaya, M. S., Smirnov, G. B., Brubaker, R. R. (1999). Expression of the Plague Plasminogen Activator in Yersinia pseudotuberculosis and Escherichia coli. Infect. Immun. 67: 1359-1367 [Abstract] [Full Text]