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Infect Immun. 1988 November; 56(11): 2866-2870

Tn916 insertional inactivation of multiple genes on the chromosome of Streptococcus mutans GS-5.

Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.

ABSTRACT

Streptococcus mutans GS-5 was transformed with the Escherichia coli plasmid pAM150 containing the cloned streptococcal transposon Tn916. Southern blot analyses with the tetracycline-resistant determinant of Tn916 showed that Tn916 was inserted into the chromosome of S. mutans at a variety of different sites. Tn916 insertions resulted in the inactivation of genes that code for various steps in the biosynthesis of several different amino acids. Two auxotrophs which contained a single copy of Tn916 were shown to revert to prototrophy at frequencies of about 10(-8). All of the revertant prototrophs were susceptible to tetracycline, indicating regeneration of the functional gene by excision of Tn916.

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