This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stoebner, J A Articles by Payne, S M Search for Related Content PubMed PubMed Citation Articles by Stoebner, J A Articles by Payne, S M

 Previous Article  |  Next Article 

Infect Immun. 1988 November; 56(11): 2891-2895

Iron-regulated hemolysin production and utilization of heme and hemoglobin by Vibrio cholerae.

Department of Microbiology, University of Texas, Austin 78712-1095.

ABSTRACT

El Tor and non-O1 strains of Vibrio cholerae were analyzed to determine whether synthesis of secreted hemolysin was influenced by the concentration of iron in the medium. Synthesis of hemolysin was found to be iron regulated in both El Tor and non-O1 isolates. Increased levels of hemolytic activity were detected in supernatants of iron-starved cells. Spontaneous hemolysin-deficient mutants of one non-O1 strain were found to occur at high frequency. These variants also failed to synthesize vibriobactin, the iron transport compound utilized by V. cholerae. Another non-O1 strain was found to synthesize both hemolysin and vibriobactin constitutively. When the cloned Escherichia coli fur gene, encoded on the plasmid pABN203, was introduced into this constitutive strain, normal iron regulation of both hemolysin and vibriobactin was reestablished. The ability of V. cholerae to utilize mammalian iron compounds was determined, and it was found that both hemin and hemoglobin could serve as sole sources of iron.

This article has been cited by other articles:

• Mey, A. R., Wyckoff, E. E., Hoover, L. A., Fisher, C. R., Payne, S. M. (2008). Vibrio cholerae VciB Promotes Iron Uptake via Ferrous Iron Transporters. J. Bacteriol. 190: 5953-5962 [Abstract] [Full Text]  
• Asuthkar, S., Velineni, S., Stadlmann, J., Altmann, F., Sritharan, M. (2007). Expression and Characterization of an Iron-Regulated Hemin-Binding Protein, HbpA, from Leptospira interrogans Serovar Lai. Infect. Immun. 75: 4582-4591 [Abstract] [Full Text]  
• Mey, A. R., Wyckoff, E. E., Kanukurthy, V., Fisher, C. R., Payne, S. M. (2005). Iron and Fur Regulation in Vibrio cholerae and the Role of Fur in Virulence. Infect. Immun. 73: 8167-8178 [Abstract] [Full Text]  
• Mey, A. R., Craig, S. A., Payne, S. M. (2005). Characterization of Vibrio cholerae RyhB: the RyhB Regulon and Role of ryhB in Biofilm Formation. Infect. Immun. 73: 5706-5719 [Abstract] [Full Text]  
• Ahn, S.-H., Han, J.-H., Lee, J.-H., Park, K.-J., Kong, I.-S. (2005). Identification of an Iron-Regulated Hemin-Binding Outer Membrane Protein, HupO, in Vibrio fluvialis: Effects on Hemolytic Activity and the Oxidative Stress Response. Infect. Immun. 73: 722-729 [Abstract] [Full Text]  
• Wyckoff, E. E., Schmitt, M., Wilks, A., Payne, S. M. (2004). HutZ Is Required for Efficient Heme Utilization in Vibrio cholerae. J. Bacteriol. 186: 4142-4151 [Abstract] [Full Text]  
• Murphy, E. R., Sacco, R. E., Dickenson, A., Metzger, D. J., Hu, Y., Orndorff, P. E., Connell, T. D. (2002). BhuR, a Virulence-Associated Outer Membrane Protein of Bordetella avium, Is Required for the Acquisition of Iron from Heme and Hemoproteins. Infect. Immun. 70: 5390-5403 [Abstract] [Full Text]  
• Mey, A. R., Wyckoff, E. E., Oglesby, A. G., Rab, E., Taylor, R. K., Payne, S. M. (2002). Identification of the Vibrio cholerae Enterobactin Receptors VctA and IrgA: IrgA Is Not Required for Virulence. Infect. Immun. 70: 3419-3426 [Abstract] [Full Text]  
• Sha, J., Lu, M., Chopra, A. K. (2001). Regulation of the Cytotoxic Enterotoxin Gene in Aeromonas hydrophila: Characterization of an Iron Uptake Regulator. Infect. Immun. 69: 6370-6381 [Abstract] [Full Text]  
• Wilks, A., Moenne-Loccoz, P. (2000). Identification of the Proximal Ligand His-20 in Heme Oxygenase (Hmu O) from Corynebacterium diphtheriae. OXIDATIVE CLEAVAGE OF THE HEME MACROCYCLE DOES NOT REQUIRE THE PROXIMAL HISTIDINE. J. Biol. Chem. 275: 11686-11692 [Abstract] [Full Text]  
• Butterton, J. R., Choi, M. H., Watnick, P. I., Carroll, P. A., Calderwood, S. B. (2000). Vibrio cholerae VibF Is Required for Vibriobactin Synthesis and Is a Member of the Family of Nonribosomal Peptide Synthetases. J. Bacteriol. 182: 1731-1738 [Abstract] [Full Text]  
• Ochsner, U. A., Johnson, Z., Vasil, M. L. (2000). Genetics and regulation of two distinct haem-uptake systems, phu and has, in Pseudomonas aeruginosa. Microbiology 146: 185-198 [Abstract] [Full Text]  
• O'Malley, S. M., Mouton, S. L., Occhino, D. A., Deanda, M. T., Rashidi, J. R., Fuson, K. L., Rashidi, C. E., Mora, M. Y., Payne, S. M., Henderson, D. P. (1999). Comparison of the Heme Iron Utilization Systems of Pathogenic Vibrios. J. Bacteriol. 181: 3594-3598 [Abstract] [Full Text]  
• Bearden, S. W., Staggs, T. M., Perry, R. D. (1998). An ABC Transporter System of Yersinia pestis Allows Utilization of Chelated Iron by Escherichia coli SAB11. J. Bacteriol. 180: 1135-1147 [Abstract] [Full Text]  
• Wilks, A., Schmitt, M. P. (1998). Expression and Characterization of a Heme Oxygenase (Hmu O) from Corynebacterium diphtheriae. IRON ACQUISITION REQUIRES OXIDATIVE CLEAVAGE OF THE HEME MACROCYCLE. J. Biol. Chem. 273: 837-841 [Abstract] [Full Text]  
• Slauch, J, Taylor, R, Maloy, S (1997). Survival in a cruel world: how Vibrio cholerae and Salmonella respond to an unwilling host.. Genes Dev. 11: 1761-1774