This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ferrante, A Search for Related Content PubMed PubMed Citation Articles by Ferrante, A

 Previous Article  |  Next Article 

Infect Immun. 1989 October; 57(10): 3110-3115

Augmentation of the neutrophil response to Naegleria fowleri by tumor necrosis factor alpha.

Department of Immunology, Adelaide Children's Hospital, South Australia.

ABSTRACT

Conditioned medium from phytohemagglutinin-stimulated human mononuclear leukocytes, previously shown to activate neutrophils for amoeba killing, was found to contain high levels of tumor necrosis factor alpha (TNF-alpha) by an enzyme-linked immunosorbent assay. The effects of human recombinant TNF-alpha on the response of human neutrophils to the pathogenic free-living amoeba Naegleria fowleri was studied in vitro. The data showed that recombinant human TNF-alpha augmented the neutrophil respiratory burst (assessed by the cytochrome c reduction assay and lucigenin-dependent chemiluminescence assay) in response to amoebae opsonized with human serum. The priming effects of TNF-alpha were transient; marked enhancement was found with short 5- to 30-min preincubations of neutrophils with the cytokine. The enhancement of oxygen radical production was evident with 20 U of TNF-alpha per 10(6) neutrophils and continued to increase with up to 100 U. TNF-alpha also augmented the neutrophil lysosomal enzyme release in response to N. fowleri. The results support previous reports suggesting an important role of neutrophil cytokine activation for effective immunity against free-living amoebae.

This article has been cited by other articles:

• Rojas-Hernandez, S., Rodriguez-Monroy, M. A., Lopez-Revilla, R., Resendiz-Albor, A. A., Moreno-Fierros, L. (2004). Intranasal Coadministration of the Cry1Ac Protoxin with Amoebal Lysates Increases Protection against Naegleria fowleri Meningoencephalitis. Infect. Immun. 72: 4368-4375 [Abstract] [Full Text]  
• Twining, S. S., Schulte, D. P., Wilson, P. M., Fish, B. L., Moulder, J. E. (1997). Vitamin A Deficiency Alters Rat Neutrophil Function. J. Nutr. 127: 558-565 [Abstract] [Full Text]