This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Barrow, P A Articles by Lovell, M A Search for Related Content PubMed PubMed Citation Articles by Barrow, P A Articles by Lovell, M A

 Previous Article  |  Next Article 

Infect Immun. 1989 October; 57(10): 3136-3141

Functional homology of virulence plasmids in Salmonella gallinarum, S. pullorum, and S. typhimurium.

AFRC Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambridgeshire, United Kingdom.

ABSTRACT

The virulence-associated plasmids of strains of Salmonella gallinarum and S. pullorum were transferred separately by mobilization with the F plasmid into virulence plasmid-cured derivatives of S. gallinarum, S. pullorum, and S. typhimurium and into a prototrophic Escherichia coli K-12 strain. The transconjugants were tested for virulence in chickens of different ages and in mice. The S. gallinarum and S. pullorum plasmids were able to restore full virulence in the three Salmonella strains, thus demonstrating functional homology in virulence plasmids from these Salmonella serotypes and biotypes. The virulence phenotypes of the transconjugants remained the same as that of the parent strain of the recipient. This, together with the fact that E. coli K-12 containing either of the virulence plasmids was avirulent for chickens, suggested that in addition to plasmid genes, chromosomal genes are important in determining virulence, particularly in determining the ability to survive and multiply in the cells of the reticuloendothelial system. The virulence plasmids were not self-transmissible and could not be transduced by temperate bacteriophages lysogenizing field strains of S. gallinarum. They were not in the same incompatibility group as F but were fi+.

This article has been cited by other articles:

• Shah, D. H., Lee, M.-j., Park, J.-h., Lee, J.-h., Eo, S.-k., Kwon, J.-t., Chae, J.-s. (2005). Identification of Salmonella gallinarum virulence genes in a chicken infection model using PCR-based signature-tagged mutagenesis. Microbiology 151: 3957-3968 [Abstract] [Full Text]  
• Chu, C., Hong, S.-F., Tsai, C., Lin, W.-S., Liu, T.-P., Ou, J. T. (1999). Comparative Physical and Genetic Maps of the Virulence Plasmids of Salmonella enterica Serovars Typhimurium, Enteritidis, Choleraesuis, and Dublin. Infect. Immun. 67: 2611-2614 [Abstract] [Full Text]