This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Carsiotis, M Articles by O'Brien, A D Search for Related Content PubMed PubMed Citation Articles by Carsiotis, M Articles by O'Brien, A D

 Previous Article  |  Next Article 

Infect Immun. 1989 November; 57(11): 3276-3280

A Salmonella typhimurium virulence gene linked to flg.

Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Ohio 45267-0524.

ABSTRACT

Isogenic pairs of strains of Salmonella typhimurium which differed only in whether or not they were flagellate were found to be equally virulent in C57BL/6J mice infected orally, intravenously, or intraperitoneally. Therefore, we investigated the genetic basis for our previous observation that in this mouse model, nonflagellate delta flagABCDE25 strains were reduced in virulence compared with isogenic wild-type flagellate strains. The recombinant plasmid pMH6, which contains several flg+ genes and a segment of the S. typhimurium chromosome adjacent to the flg genes, was introduced into a delta flgABCDE25 mutant. This restored virulence in mice challenged intraperitoneally, which suggested that a virulence gene occurs adjacent to the flg genes. When plasmid pMH64, which lacks the chromosomal segment adjacent to the flg genes, was introduced into the same delta flgABCDE25 mutant, virulence was not restored. In contrast, the introduction of pMH71, a plasmid which retains the chromosomal segment adjacent to the flg genes, restored virulence. We concluded that a hitherto unknown virulence gene, which we have named mviS, occurs adjacent to the flg genes and that its absence in delta flgABCDE25 mutants, rather than the nonflagellate phenotype of the delta flgABCDE25 mutants, caused the previously reported attenuation of such mutants.

This article has been cited by other articles:

• Salazar-Gonzalez, R.-M., Srinivasan, A., Griffin, A., Muralimohan, G., Ertelt, J. M., Ravindran, R., Vella, A. T., McSorley, S. J. (2007). Salmonella Flagellin Induces Bystander Activation of Splenic Dendritic Cells and Hinders Bacterial Replication In Vivo. J. Immunol. 179: 6169-6175 [Abstract] [Full Text]  
• Radosevich, T. J., Reinhardt, T. A., Lippolis, J. D., Bannantine, J. P., Stabel, J. R. (2007). Proteome and Differential Expression Analysis of Membrane and Cytosolic Proteins from Mycobacterium avium subsp. paratuberculosis Strains K-10 and 187. J. Bacteriol. 189: 1109-1117 [Abstract] [Full Text]  
• Ducey, T. F., Carson, M. B., Orvis, J., Stintzi, A. P., Dyer, D. W. (2005). Identification of the Iron-Responsive Genes of Neisseria gonorrhoeae by Microarray Analysis in Defined Medium. J. Bacteriol. 187: 4865-4874 [Abstract] [Full Text]  
• Fisher, M. A., Grimm, D., Henion, A. K., Elias, A. F., Stewart, P. E., Rosa, P. A., Gherardini, F. C. (2005). From the Cover: Borrelia burgdorferi {sigma}54 is required for mammalian infection and vector transmission but not for tick colonization. Proc. Natl. Acad. Sci. USA 102: 5162-5167 [Abstract] [Full Text]  
• McLeod, M. P., Qin, X., Karpathy, S. E., Gioia, J., Highlander, S. K., Fox, G. E., McNeill, T. Z., Jiang, H., Muzny, D., Jacob, L. S., Hawes, A. C., Sodergren, E., Gill, R., Hume, J., Morgan, M., Fan, G., Amin, A. G., Gibbs, R. A., Hong, C., Yu, X.-j., Walker, D. H., Weinstock, G. M. (2004). Complete Genome Sequence of Rickettsia typhi and Comparison with Sequences of Other Rickettsiae. J. Bacteriol. 186: 5842-5855 [Abstract] [Full Text]  
• Schmitt, C. K., Ikeda, J. S., Darnell, S. C., Watson, P. R., Bispham, J., Wallis, T. S., Weinstein, D. L., Metcalf, E. S., O'Brien, A. D. (2001). Absence of All Components of the Flagellar Export and Synthesis Machinery Differentially Alters Virulence of Salmonella enterica Serovar Typhimurium in Models of Typhoid Fever, Survival in Macrophages, Tissue Culture Invasiveness, and Calf Enterocolitis. Infect. Immun. 69: 5619-5625 [Abstract] [Full Text]  
• Ikeda, J. S., Schmitt, C. K., Darnell, S. C., Watson, P. R., Bispham, J., Wallis, T. S., Weinstein, D. L., Metcalf, E. S., Adams, P., O'Connor, C. D., O'Brien, A. D. (2001). Flagellar Phase Variation of Salmonella enterica Serovar Typhimurium Contributes to Virulence in the Murine Typhoid Infection Model but Does Not Influence Salmonella-Induced Enteropathogenesis. Infect. Immun. 69: 3021-3030 [Abstract] [Full Text]  
• Rudnick, P. A., Arcondéguy, T., Kennedy, C. K., Kahn, D. (2001). glnD and mviN Are Genes of an Essential Operon in Sinorhizobium meliloti. J. Bacteriol. 183: 2682-2685 [Abstract] [Full Text]  
• Eichelberg, K., Galan, J. E. (2000). The Flagellar Sigma Factor FliA (sigma 28) Regulates the Expression of Salmonella Genes Associated with the Centisome 63 Type III Secretion System. Infect. Immun. 68: 2735-2743 [Abstract] [Full Text]