This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arthur, M Articles by Goldstein, R Search for Related Content PubMed PubMed Citation Articles by Arthur, M Articles by Goldstein, R

 Previous Article  |  Next Article 

Infect Immun. 1989 February; 57(2): 314-321

Structure and copy number of gene clusters related to the pap P-adhesin operon of uropathogenic Escherichia coli.

Maxwell Finland Laboratory for Infectious Diseases, Boston University School of Medicine, Massachusetts 02118.

ABSTRACT

The structurally related pap and prs operons of the uropathogenic Escherichia coli isolate J96 encode a P and an F adhesin that mediate bacterial attachment to the human P blood group antigen and the Forssman antigen, respectively. Using probes prepared from different segments of the pap operon, Southern blot hybridizations were performed to characterize pap-related sequences of 30 E. coli clinical isolates expressing different adhesin phenotypes. Gene clusters encoding P and F adhesins displayed no restriction site polymorphism in sequences homologous to the papH, papC, and papD genes that encode proteins essential to the transport and polymerization of the subunits of the P-pilus adhesin. In contrast, pap-related genetic elements associated with a null phenotype either lacked homology to the papH, papC, and papD genes or displayed a restriction site polymorphism in this region. Sequences within and surrounding the J96 papG and prsG adhesin genes that determine the binding specificities to the P and F antigens, respectively, were not conserved. However, gene clusters encoding different binding specificities could not be distinguished based on such restriction site polymorphisms. The majority of clinical isolates had more than one copy of pap-related sequences that involved gene clusters similar to the J96 pap operon, as well as genetic elements that were related only to a part of this operon. The implications of this unexpected copy number polymorphism with respect to possible recombination events involving pap-related sequences are discussed.

This article has been cited by other articles:

• Bolduc, G. R., Bouchet, V., Jiang, R.-Z., Geisselsoder, J., Truong-Bolduc, Q. C., Rice, P. A., Pelton, S. I., Goldstein, R. (2000). Variability of Outer Membrane Protein P1 and Its Evaluation as a Vaccine Candidate against Experimental Otitis Media due to Nontypeable Haemophilus influenzae: an Unambiguous, Multifaceted Approach. Infect. Immun. 68: 4505-4517 [Abstract] [Full Text]  
• Johnson, J. R., Brown, J. J., Ahmed, P. (1998). Diversity of Hemagglutination Phenotypes among P-Fimbriated Wild-Type Strains of Escherichia coli in Relation to papG Allele Repertoire. CVI 5: 160-170 [Abstract] [Full Text]  
• Steinbach, S., Sun, L., Jiang, R.-Z., Flume, P., Gilligan, P., Egan, T. M., Goldstein, R. (1994). Transmissibility of Pseudomonas cepacia Infection in Clinic Patients and Lung-Transplant Recipients with Cystic Fibrosis. NEJM 331: 981-987 [Abstract] [Full Text]