This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Clinkenbeard, K D
Right arrow Articles by Confer, A W
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Clinkenbeard, K D
Right arrow Articles by Confer, A W

 Previous Article  |  Next Article 

Infect Immun. 1989 February; 57(2): 420-425

Transmembrane pore size and role of cell swelling in cytotoxicity caused by Pasteurella haemolytica leukotoxin.

K D Clinkenbeard, D A Mosier and A W Confer

Department of Veterinary Pathology, Oklahoma State University, Stillwater 74078.

ABSTRACT

Pasteurella haemolytica A1 leukotoxin causes rapid (5 to 15 min) leakage of intracellular K+ and cell swelling and slower (15 to 60 min), Ca2+-dependent formation of large plasma membrane defects (congruent to 100 nm) and leakage of lactate dehydrogenase from bovine lymphoma cells (BL3 cells) (K. D. Clinkenbeard, D. A. Mosier, A. L. Timko, and A. W. Confer, Am. J. Vet. Res., in press). Incubation of BL3 cells in medium made hypertonic by inclusion of 75 mM sucrose blocked leukotoxin-induced cell swelling, formation of large plasma membrane defects, and leakage of lactate dehydrogenase but did not block leukotoxin-induced leakage of intracellular K+. Carbohydrates with molecular weights less than that of sucrose, e.g., mannitol, did not block leukotoxin-induced cell swelling of BL3 cells. Increasing the concentration of mannitol to twice that of sucrose still resulted in no protective effect. Assuming that leukotoxin acts as a transmembrane molecular sieve, then the functional transmembrane pore size formed by leukotoxin in BL3 cells is slightly less than the size of sucrose, i.e., 0.9 nm. Exposure of BL3 cells to leukotoxin for 15 or 45 min followed by the addition of hypertonic sucrose to the incubation medium reversed leukotoxin-induced cell swelling and prevented further leakage of lactate dehydrogenase. Leukotoxin-induced leakage of lactate dehydrogenase required both cell swelling and Ca2+-dependent processes. The Ca2+-dependent steps can occur before or concurrent with cell swelling.

Infect Immun. 1989 February; 57(2): 420-425




This article has been cited by other articles:

  • Wiles, T. J., Dhakal, B. K., Eto, D. S., Mulvey, M. A. (2008). Inactivation of Host Akt/Protein Kinase B Signaling by Bacterial Pore-forming Toxins. Mol. Biol. Cell 19: 1427-1438 [Abstract] [Full Text]  
  • Narayanan, S., Stewart, G. C., Chengappa, M. M., Willard, L., Shuman, W., Wilkerson, M., Nagaraja, T. G. (2002). Fusobacterium necrophorum Leukotoxin Induces Activation and Apoptosis of Bovine Leukocytes. Infect. Immun. 70: 4609-4620 [Abstract] [Full Text]  
  • Lee, R. W. H., Strommer, J., Hodgins, D., Shewen, P. E., Niu, Y., Lo, R. Y. C. (2001). Towards Development of an Edible Vaccine against Bovine Pneumonic Pasteurellosis Using Transgenic White Clover Expressing a Mannheimia haemolytica A1 Leukotoxin 50 Fusion Protein. Infect. Immun. 69: 5786-5793 [Abstract] [Full Text]  
  • Highlander, S. K., Fedorova, N. D., Dusek, D. M., Panciera, R., Alvarez, L. E., Rinehart, C. (2000). Inactivation of Pasteurella (Mannheimia) haemolytica Leukotoxin Causes Partial Attenuation of Virulence in a Calf Challenge Model. Infect. Immun. 68: 3916-3922 [Abstract] [Full Text]  
  • Gilbert, R. O., Elia, G., Beach, D. H., Klaessig, S., Singh, B. N. (2000). Cytopathogenic Effect of Trichomonas vaginalis on Human Vaginal Epithelial Cells Cultured In Vitro. Infect. Immun. 68: 4200-4206 [Abstract] [Full Text]  
  • Billson, F. M., Harbour, C., Michalski, W. P., Tennent, J. M., Egerton, J. R., Hodgson, J. L. (2000). Characterization of Hemolysin of Moraxella bovis Using a Hemolysis-Neutralizing Monoclonal Antibody. Infect. Immun. 68: 3469-3474 [Abstract] [Full Text]  
  • Sun, Y., Clinkenbeard, K. D., Cudd, L. A., Clarke, C. R., Clinkenbeard, P. A. (1999). Correlation of Pasteurella haemolytica Leukotoxin Binding with Susceptibility to Intoxication of Lymphoid Cells from Various Species. Infect. Immun. 67: 6264-6269 [Abstract] [Full Text]  
  • Singh, B. N., Lucas, J. J., Beach, D. H., Shin, S. T., Gilbert, R. O. (1999). Adhesion of Tritrichomonas foetus to Bovine Vaginal Epithelial Cells. Infect. Immun. 67: 3847-3854 [Abstract] [Full Text]  
  • Whiteley, L. O., Maheswaran, S. K., Weiss, D. J., Ames, T. R. (1991). Alterations in Pulmonary Morphology and Peripheral Coagulation Profiles Caused by Intratracheal Inoculation of Live and Ultraviolet Light-killed Pasteurella haemolytica A1 in Calves. Veterinary Pathology 28: 275-285 [Abstract]  
  • Whiteley, L. O., Maheswaran, S. K., Weiss, D. J., Ames, T. R. (1990). Immunohistochemical Localization of Pasteurella haemolytica Al-derived Endotoxin, Leukotoxin, and Capsular Polysaccharide in Experimental Bovine Pasteurella Pneumonia. Veterinary Pathology 27: 150-161 [Abstract]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»