This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yamashita, T Articles by Saito, K Search for Related Content PubMed PubMed Citation Articles by Yamashita, T Articles by Saito, K

 Previous Article  |  Next Article 

Infect Immun. 1989 August; 57(8): 2405-2409

Purification, primary structure, and biological activity of guinea pig neutrophil cationic peptides.

Department of Biochemistry, School of Medicine, Juntendo University, Tokyo, Japan.

ABSTRACT

The guinea pig neutrophil cationic peptides GNCP-1 and GNCP-2 were purified from a granule-rich subcellular fraction of peritoneal exudate neutrophils by acid-gel electrophoresis and reversed-phase high-performance liquid chromatography. Both peptides not only released histamine from rat mast cells to the same extent but also were equally active against Staphylococcus aureus and Escherichia coli. The peptides were rich in arginine and cystine and lacked free sulfhydryl groups. Composition and sequence determinations revealed that GNCP-1 and GNCP-2 are each single polypeptides containing 31 amino acid residues and three intramolecular disulfide bonds. The complete amino acid sequence of GNCP-1 is Arg-Arg-Cys-Ile-Cys-Thr-Thr-Arg-Thr-Cys-Arg-Phe-Pro-Tyr-Arg-Arg-Leu-Gly- Thr-Cys - Ile-Phe-Gln-Asn-Arg-Val-Tyr-Thr-Phe-Cys-Cys. The sequence of GNCP-2 is identical except for the substitution of isoleucine for leucine at residue 21.

This article has been cited by other articles:

• Diamond, G., Beckloff, N., Ryan, L.K. (2008). Host Defense Peptides in the Oral Cavity and the Lung: Similarities and Differences. J. Dent. Res. 87: 915-927 [Abstract] [Full Text]  
• Biragyn, A., Coscia, M., Nagashima, K., Sanford, M., Young, H. A., Olkhanud, P. (2008). Murine {beta}-defensin 2 promotes TLR-4/MyD88-mediated and NF-{kappa}B-dependent atypical death of APCs via activation of TNFR2. J. Leukoc. Biol. 83: 998-1008 [Abstract] [Full Text]  
• Rank, R. G., Bowlin, A. K., Reed, R. L., Darville, T. (2003). Characterization of Chlamydial Genital Infection Resulting from Sexual Transmission from Male to Female Guinea Pigs and Determination of Infectious Dose. Infect. Immun. 71: 6148-6154 [Abstract] [Full Text]  
• Biragyn, A., Surenhu, M., Yang, D., Ruffini, P. A., Haines, B. A., Klyushnenkova, E., Oppenheim, J. J., Kwak, L. W. (2001). Mediators of Innate Immunity That Target Immature, But Not Mature, Dendritic Cells Induce Antitumor Immunity When Genetically Fused with Nonimmunogenic Tumor Antigens. J. Immunol. 167: 6644-6653 [Abstract] [Full Text]  
• Yang, D., Chertov, O., Oppenheim, J. J. (2001). Participation of mammalian defensins and cathelicidins in anti-microbial immunity: receptors and activities of human defensins and cathelicidin (LL-37). J. Leukoc. Biol. 69: 691-697 [Abstract] [Full Text]  
• Halder, T. M., Bluggel, M., Heinzel, S., Pawelec, G., Meyer, H. E., Kalbacher, H. (2000). Defensins are dominant HLA-DR-associated self-peptides from CD34- peripheral blood mononuclear cells of different tumor patients (plasmacytoma, chronic myeloid leukemia). Blood 95: 2890-2896 [Abstract] [Full Text]  
• Befus, A. D., Mowat, C., Gilchrist, M., Hu, J., Solomon, S., Bateman, A. (1999). Neutrophil Defensins Induce Histamine Secretion from Mast Cells: Mechanisms of Action. J. Immunol. 163: 947-953 [Abstract] [Full Text]  
• van den Berg, R. H., Faber-Krol, M. C., van Wetering, S., Hiemstra, P. S., Daha, M. R. (1998). Inhibition of Activation of the Classical Pathway of Complement by Human Neutrophil Defensins. Blood 92: 3898-3903 [Abstract] [Full Text]  
• Gamelli, R. L., He, L.-K., Liu, H., Ricken, J. D. (1998). Improvement in Survival With Peptidyl Membrane Interactive Molecule D4B Treatment After Burn Wound Infection. Arch Surg 133: 715-720 [Abstract] [Full Text]  
• Bateman, A., MacLeod, R. J., Lembessis, P., Hu, J., Esch, F., Solomon, S. (1996). The Isolation and Characterization of a Novel Corticostatin/Defensin-like Peptide from the Kidney. J. Biol. Chem. 271: 10654-10659 [Abstract] [Full Text]