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Infect Immun. 1989 September; 57(9): 2919-2922

Internalization of Shigella flexneri into HeLa cells occurs without an increase in cytosolic Ca2+ concentration.

P L Clerc, B Berthon, M Claret and P J Sansonetti

Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, France.

ABSTRACT

Pathogenic Shigella flexneri invades epithelial cells through directed phagocytosis. The role of intracellular free calcium ([Ca2+]i) as a signal for this process was tested. No variation in the [Ca2+]i could be detected by using the fluorescent indicator fura 2 to measure the [Ca2+]i in HeLa cells during the invasion process. In addition, neither clamping nor maximal decreasing or increasing of the [Ca2+]i of HeLa cells had any effect on their susceptibility to invasion. These data demonstrate that [Ca2+]i is not a signal for S. flexneri directed phagocytosis in HeLa cells.

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Infect Immun. 1989 September; 57(9): 2919-2922

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