This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cover, T L
Right arrow Articles by Blaser, M J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cover, T L
Right arrow Articles by Blaser, M J

 Previous Article  |  Next Article 

Infect Immun. 1990 March; 58(3): 603-610

Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity.

T L Cover, C P Dooley and M J Blaser

Infectious Disease Section, Veterans Administration Medical Center, Denver, Colorado 80220.

ABSTRACT

Helicobacter pylori infection is strongly associated with histologic gastritis and peptic ulcer disease. Broth culture supernatants from a subset of H. pylori strains induce vacuolization in cultured cells, a phenomenon that has been attributed to cytotoxin activity. Concentrated culture supernatants from 15 of 28 (53.6%) H. pylori strains tested induced vacuolization in HeLa cells in titers ranging from 1:10 to 1:180. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining of supernatants from these 28 strains and 2 control strains demonstrated an 82-kilodalton (kDa) protein band in 3 of 16 supernatants with vacuolizing activity, but in none of 14 supernatants without vacuolizing activity. By immunoblotting with human sera, a 128-kDa band was recognized in all 16 supernatants with vacuolizing activity, compared with 9 of 14 (64%) supernatants without vacuolizing activity (P = 0.014). Serologic recognition of the 128-kDa band in H. pylori culture supernatants was more prevalent among persons infected with vacuolizing H. pylori strains than among persons infected with nonvacuolizing strains, but the difference was not statistically significant (80 versus 45%; P = 0.079); human serologic recognition of the 82-kDa band was less common. The 128-kDa band was recognized by 100% of 31 serum samples from H. pylori-infected patients with duodenal ulcer disease, compared with 60.8% of 74 serum samples from H. pylori-infected persons without peptic ulcer disease (P = 0.0001). These data indicate that antigenic 128- and 82-kDa proteins are present in H. pylori broth culture supernatants with vacuolizing activity and that serologic responses to the 128-kDa protein are more prevalent among H. pylori-infected persons with duodenal ulceration than among infected persons without peptic ulceration.

Infect Immun. 1990 March; 58(3): 603-610




This article has been cited by other articles:

  • Lippman, S. M., Hawk, E. T. (2009). Cancer Prevention: From 1727 to Milestones of the Past 100 Years. Cancer Res. 69: 5269-5284 [Abstract] [Full Text]  
  • Lippman, S. M. (2009). Cancer Prevention Research: Back to the Future. Cancer Prevention Research 2: 503-513 [Full Text]  
  • Chitcholtan, K., Hampton, M. B., Keenan, J. I. (2008). Outer membrane vesicles enhance the carcinogenic potential of Helicobacter pylori. Carcinogenesis 29: 2400-2405 [Abstract] [Full Text]  
  • Keenan, J. I., Davis, K. A., Beaugie, C. R., McGovern, J. J., Moran, A. P. (2008). Alterations in Helicobacter pylori outer membrane and outer membrane vesicle-associated lipopolysaccharides under iron-limiting growth conditions. Innate Immunity 14: 279-290 [Abstract]  
  • Peek, R. M. Jr (2008). Review: Prevention of gastric cancer: When is treatment of Helicobacter pylori warranted?. Therapeutic Advances in Gastroenterology 1: 19-31 [Abstract]  
  • Ayala, G., Torres-Mena, J., Lopez-Carrillo, L. (2008). Association between Helicobacter pylori VacA antigens and gastric cancer depends on the detection method used: immunoblot versus neutralization of the vacuolating activity of VacA. J Med Microbiol 57: 9-14 [Abstract] [Full Text]  
  • Megraud, F., Lehours, P. (2007). Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin. Microbiol. Rev. 20: 280-322 [Abstract] [Full Text]  
  • Bhat, N., Gaensbauer, J., Peek, R. M., Bloch, K., Tham, K.-T., Blaser, M. J., Perez-Perez, G. (2005). Local and Systemic Immune and Inflammatory Responses to Helicobacter pylori Strains. CVI 12: 1393-1400 [Abstract] [Full Text]  
  • Correa, P., Schneider, B. G. (2005). Etiology of Gastric Cancer: What Is New?. Cancer Epidemiol. Biomarkers Prev. 14: 1865-1868 [Abstract] [Full Text]  
  • Peek, R. M. Jr. (2005). Orchestration of Aberrant Epithelial Signaling by Helicobacter pylori CagA. Sci Signal 2005: pe14-pe14 [Abstract] [Full Text]  
  • Bland, M. V., Ismail, S., Heinemann, J. A., Keenan, J. I. (2004). The Action of Bismuth against Helicobacter pylori Mimics but Is Not Caused by Intracellular Iron Deprivation. Antimicrob. Agents Chemother. 48: 1983-1988 [Abstract] [Full Text]  
  • Saribasak, H., Salih, B. A., Yamaoka, Y., Sander, E. (2004). Analysis of Helicobacter pylori Genotypes and Correlation with Clinical Outcome in Turkey. J. Clin. Microbiol. 42: 1648-1651 [Abstract] [Full Text]  
  • Ismail, S., Hampton, M. B., Keenan, J. I. (2003). Helicobacter pylori Outer Membrane Vesicles Modulate Proliferation and Interleukin-8 Production by Gastric Epithelial Cells. Infect. Immun. 71: 5670-5675 [Abstract] [Full Text]  
  • Letley, D. P., Rhead, J. L., Twells, R. J., Dove, B., Atherton, J. C. (2003). Determinants of Non-toxicity in the Gastric Pathogen Helicobacter pylori. J. Biol. Chem. 278: 26734-26741 [Abstract] [Full Text]  
  • Kim, N., Weeks, D. L., Shin, J. M., Scott, D. R., Young, M. K., Sachs, G. (2002). Proteins Released by Helicobacter pylori In Vitro. J. Bacteriol. 184: 6155-6162 [Abstract] [Full Text]  
  • Xu, Q., Morgan, R. D., Roberts, R. J., Xu, S. Y., van Doorn, L. J., Donahue, J. P., Miller, G. G., Blaser, M. J. (2002). Functional analysis of iceA1, a CATG-recognizing restriction endonuclease gene in Helicobacter pylori. Nucleic Acids Res 30: 3839-3847 [Abstract] [Full Text]  
  • FIGUEROA, G., TRONCOSO, M., TOLEDO, M.S., FAUNDEZ, G., ACUNA, R. (2002). Prevalence of serum antibodies to Helicobacter pylori VacA and CagA and gastric diseases in Chile. J Med Microbiol 51: 300-304 [Abstract] [Full Text]  
  • Perez-Perez, G I, Salomaa, A, Kosunen, T U, Daverman, B, Rautelin, H, Aromaa, A, Knekt, P, Blaser, M J (2002). Evidence that cagA+Helicobacter pylori strains are disappearing more rapidly than cagA- strains. Gut 50: 295-298 [Abstract] [Full Text]  
  • Ando, T., Peek, R. M. Jr., Lee, Y.-C., Krishna, U., Kusugami, K., Blaser, M. J. (2002). Host Cell Responses to Genotypically Similar Helicobacter pylori Isolates from United States and Japan. CVI 9: 167-175 [Abstract] [Full Text]  
  • Lorente, S, Doiz, O, Serrano, M T., Castillo, J, Lanas, A (2002). Helicobacter pylori stimulates pepsinogen secretion from isolated human peptic cells. Gut 50: 13-18 [Abstract] [Full Text]  
  • Chiou, C-C, Chan, C-C, Sheu, D-L, Chen, K-T, Li, Y-S, Chan, E-C (2001). Helicobacter pylori infection induced alteration of gene expression in human gastric cells. Gut 48: 598-604 [Abstract] [Full Text]  
  • Peek, R. M. Jr., van Doorn, L.-J., Donahue, J. P., Tham, K. T., Figueiredo, C., Blaser, M. J., Miller, G. G. (2000). Quantitative Detection of Helicobacter pylori Gene Expression In Vivo and Relationship to Gastric Pathology. Infect. Immun. 68: 5488-5495 [Abstract] [Full Text]  
  • Ando, T., Perez-Perez, G. I., Kusugami, K., Ohsuga, M., Bloch, K. C., Blaser, M. J. (2000). Anti-CagA Immunoglobulin G Responses Correlate with Interleukin-8 Induction in Human Gastric Mucosal Biopsy Culture. CVI 7: 803-809 [Abstract] [Full Text]  
  • Smythies, L. E., Waites, K. B., Lindsey, J. R., Harris, P. R., Ghiara, P., Smith, P. D. (2000). Helicobacter pylori-Induced Mucosal Inflammation Is Th1 Mediated and Exacerbated in IL-4, But Not IFN-{gamma}, Gene-Deficient Mice. J. Immunol. 165: 1022-1029 [Abstract] [Full Text]  
  • Beil, W., Enss, M. L., Müller, S., Obst, B., Sewing, K.-F., Wagner, S. (2000). Role of vacA and cagA in Helicobacter pylori Inhibition of Mucin Synthesis in Gastric Mucous Cells. J. Clin. Microbiol. 38: 2215-2218 [Abstract] [Full Text]  
  • Kather, B., Stingl, K., van der Rest, M. E., Altendorf, K., Molenaar, D. (2000). Another Unusual Type of Citric Acid Cycle Enzyme in Helicobacter pylori: the Malate:Quinone Oxidoreductase. J. Bacteriol. 182: 3204-3209 [Abstract] [Full Text]  
  • Spechler, S. J., Fischbach, L., Feldman, M. (2000). Clinical Aspects of Genetic Variability in Helicobacter pylori. JAMA 283: 1264-1266 [Full Text]  
  • Asahi, M., Azuma, T., Ito, S., Ito, Y., Suto, H., Nagai, Y., Tsubokawa, M., Tohyama, Y., Maeda, S., Omata, M., Suzuki, T., Sasakawa, C. (2000). Helicobacter pylori Caga Protein Can Be Tyrosine Phosphorylated in Gastric Epithelial Cells. JEM 191: 593-602 [Abstract] [Full Text]  
  • Ito, Y., Azuma, T., Ito, S., Suto, H., Miyaji, H., Yamazaki, Y., Kato, T., Kohli, Y., Keida, Y., Kuriyama, M. (2000). Sequence Analysis and Clinical Significance of the iceA Gene from Helicobacter pylori Strains in Japan. J. Clin. Microbiol. 38: 483-488 [Abstract] [Full Text]  
  • Kato, S., Sugiyama, T., Kudo, M., Ohnuma, K., Ozawa, K., Iinuma, K., Asaka, M., Blaser, M. J. (2000). CagA Antibodies in Japanese Children with Nodular Gastritis or Peptic Ulcer Disease. J. Clin. Microbiol. 38: 68-70 [Abstract] [Full Text]  
  • Kidd, M, Lastovica, A J, Atherton, J C, Louw, J A (1999). Heterogeneity in the Helicobacter pylori vacA and cagA genes: association with gastroduodenal disease in South Africa?. Gut 45: 499-502 [Abstract] [Full Text]  
  • Morales-Espinosa, R., Castillo-Rojas, G., Gonzalez-Valencia, G., Ponce de León, S., Cravioto, A., Atherton, J. C., López-Vidal, Y. (1999). Colonization of Mexican Patients by Multiple Helicobacter pylori Strains with Different vacA and cagA Genotypes. J. Clin. Microbiol. 37: 3001-3004 [Abstract] [Full Text]  
  • Marais, A., Mendz, G. L., Hazell, S. L., Megraud, F. (1999). Metabolism and Genetics of Helicobacter pylori: the Genome Era. Microbiol. Mol. Biol. Rev. 63: 642-674 [Abstract] [Full Text]  
  • ZEVERING, Y, JACOB, L, MEYER, T F (1999). Naturally acquired human immune responses against Helicobacter pylori and implications for vaccine development. Gut 45: 465-474 [Full Text]  
  • Ng, E. K. W., Thompson, S. A., Perez-Perez, G. I., Kansau, I., van der Ende, A., Labigne, A., Sung, J. J. Y., Chung, S. C. S., Blaser, M. J. (1999). Helicobacter pylori Heat Shock Protein A: Serologic Responses and Genetic Diversity. CVI 6: 377-382 [Abstract] [Full Text]  
  • Donati, M., Storni, E., D'Apote, L., Moreno, S., Tucci, A., Poli, L., Cevenini, R. (1999). PCR-Based Restriction Pattern Typing of the vacA Gene Provides Evidence for a Homogeneous Group among Helicobacter pylori Strains Associated with Peptic Ulcer Disease. J. Clin. Microbiol. 37: 912-915 [Abstract] [Full Text]  
  • Yamaoka, Y., Kodama, T., Graham, D. Y., Kashima, K. (1998). Comparison of Four Serological Tests To Determine the CagA or VacA Status of Helicobacter pylori Strains. J. Clin. Microbiol. 36: 3433-3434 [Abstract] [Full Text]  
  • Sommi, P., Ricci, V., Fiocca, R., Necchi, V., Romano, M., Telford, J. L., Solcia, E., Ventura, U. (1998). Persistence of Helicobacter pylori VacA toxin and vacuolating potential in cultured gastric epithelial cells. Am. J. Physiol. Gastrointest. Liver Physiol. 275: G681-G688 [Abstract] [Full Text]  
  • Terres, A. M., Pajares, J. M., Hopkins, A. M., Murphy, A., Moran, A., Baird, A. W., Kelleher, D. (1998). Helicobacter pylori Disrupts Epithelial Barrier Function in a Process Inhibited by Protein Kinase C Activators. Infect. Immun. 66: 2943-2950 [Abstract] [Full Text]  
  • Lehmann, F. S., Schiller, N., Cover, T., Hatch, R., Seensalu, R., Kato, K., Walsh, J. H., Soll, A. H. (1998). H. pylori stimulates gastrin release from canine antral cells in primary culture. Am. J. Physiol. Gastrointest. Liver Physiol. 274: G992-G996 [Abstract] [Full Text]  
  • Rudi, J., Kolb, C., Maiwald, M., Kuck, D., Sieg, A., Galle, P. R., Stremmel, W. (1998). Diversity of Helicobacter pylori vacA and cagA Genes and Relationship to VacA and CagA Protein Expression, Cytotoxin Production, and Associated Diseases. J. Clin. Microbiol. 36: 944-948 [Abstract] [Full Text]  
  • Maeda, S, Ogura, K, Yoshida, H, Kanai, F, Ikenoue, T, Kato, N, Shiratori, Y, Omata, M (1998). Major virulence factors, VacA and CagA, are commonly positive in Helicobacter pylori isolates in Japan. Gut 42: 338-343 [Abstract] [Full Text]  
  • Sharma, S. A., Tummuru, M. K. R., Blaser, M. J., Kerr, L. D. (1998). Activation of IL-8 Gene Expression by Helicobacter pylori Is Regulated by Transcription Factor Nuclear Factor-{kappa}B in Gastric Epithelial Cells. J. Immunol. 160: 2401-2407 [Abstract] [Full Text]  
  • Ogura, K, Kanai, F, Maeda, S, Yoshida, H, Ogura, M, Lan, K-H, Hirota, K, Kawabe, T, Shiratori, Y, Omata, M (1997). High prevalence of cytotoxin positive Helicobacter pylori in patients unrelated to the presence of peptic ulcers in Japan. Gut 41: 463-468 [Abstract] [Full Text]  
  • Tompkins, L., Falkow, S (1995). The new path to preventing ulcers. Science 267: 1621-1622  
  • Xu, Q., Stickel, S., Roberts, R. J., Blaser, M. J., Morgan, R. D. (2000). Purification of the Novel Endonuclease, Hpy188I, and Cloning of Its Restriction-Modification Genes Reveal Evidence of Its Horizontal Transfer to the Helicobacter pylori Genome. J. Biol. Chem. 275: 17086-17093 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»