This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kar, S Articles by Brinton, C C Search for Related Content PubMed PubMed Citation Articles by Kar, S Articles by Brinton, C C, Jr

 Previous Article  |  Next Article 

Infect Immun. 1990 April; 58(4): 903-908

Cloning and expression in Escherichia coli of LKP pilus genes from a nontypeable Haemophilus influenzae strain.

Department of Biological Sciences, University of Pittsburgh, Pennsylvania.

ABSTRACT

Nontypeable Haemophilus influenzae HF0295, isolated by aspiration from the middle ear of a patient with otitis media, expresses long, thick, and hemagglutinating pili of a single serotype (LKP1) on its surface. An intact pilus vaccine consisting of the LKP1 serotype protected chinchillas against experimental otitis media (C. C. Brinton, Jr., M. J. Carter, D. B. Derber, S. Kar, J. A. Kramarik, A. C. C. To, S. C. M. To, and S. W. Wood, Pediatr. Infect. Dis. J. 8:554-561, 1989; R. B. Karasic, D. J. Beste, S. C. M. To, W. J. Doyle, S. W. Wood, M. J. Carter, A. C. C. To, K. Tanpowpong, C. D. Bluestone, and C. C. Brinton, Jr., Pediatr. Infect. Dis. J. 8:562-565, 1989). The genes encoding LKP1 pili were cloned from a genomic library of the clinical strain as a 12.5-kilobase insert on a plasmid vector and inserted into Escherichia coli K-12. Transposon mutagenesis and deletion constructs mapped the pilus-coding region within a 7-kilobase region of insert DNA. The recombinant bacteria were found by electron microscopy to express pili morphologically similar to LKP1 pili. Purified pilus rods from the recombinant and its parental strain were composed of a single detectable protein with an apparent molecular weight of 27,500. Antibodies raised against LKP1 pili purified from H. influenzae immunologically reacted with pili from the recombinant bacteria. Pili from both strains also adhered to human erythrocytes and buccal cells with the same specificity.

This article has been cited by other articles:

• Ruer, S., Stender, S., Filloux, A., de Bentzmann, S. (2007). Assembly of Fimbrial Structures in Pseudomonas aeruginosa: Functionality and Specificity of Chaperone-Usher Machineries. J. Bacteriol. 189: 3547-3555 [Abstract] [Full Text]  
• Holm, M. M., Vanlerberg, S. L., Sledjeski, D. D., Lafontaine, E. R. (2003). The Hag Protein of Moraxella catarrhalis Strain O35E Is Associated with Adherence to Human Lung and Middle Ear Cells. Infect. Immun. 71: 4977-4984 [Abstract] [Full Text]  
• Sethi, S., Murphy, T. F. (2001). Bacterial Infection in Chronic Obstructive Pulmonary Disease in 2000: a State-of-the-Art Review. Clin. Microbiol. Rev. 14: 336-363 [Abstract] [Full Text]  
• Read, T. D., Satola, S. W., Farley, M. M. (2000). Nucleotide Sequence Analysis of Hypervariable Junctions of Haemophilus influenzae Pilus Gene Clusters. Infect. Immun. 68: 6896-6902 [Abstract] [Full Text]  
• Read, T. D., Satola, S. W., Opdyke, J. A., Farley, M. M. (1998). Copy Number of Pilus Gene Clusters in Haemophilus influenzae and Variation in the hifE Pilin Gene. Infect. Immun. 66: 1622-1631 [Abstract] [Full Text]  
• Clemans, D. L., Marrs, C. F., Patel, M., Duncan, M., Gilsdorf, J. R. (1998). Comparative Analysis of Haemophilus influenzae hifA (Pilin) Genes. Infect. Immun. 66: 656-663 [Abstract] [Full Text]