This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lo, R Y Articles by Cooney, B J Search for Related Content PubMed PubMed Citation Articles by Lo, R Y Articles by Cooney, B J

 Previous Article  |  Next Article 

Infect Immun. 1991 October; 59(10): 3398-3406

Molecular studies of Ssa1, a serotype-specific antigen of Pasteurella haemolytica A1.

Department of Microbiology, University of Guelph, Ontario, Canada.

ABSTRACT

A serotype-specific antigen of Pasteurella haemolytica A1 encoded on the recombinant plasmid pSSA1 is characterized. Nucleotide sequence analysis of the insert DNA in pSSA1 identified the gene ssaI, which codes for a protein of approximately 100 kDa. In vivo labeling of pSSA1-encoded protein in Escherichia coli maxicells showed the expression of a 100-kDa protein from the insert DNA on the recombinant plasmid. Northern blot and primer extension analyses were used to identify the mRNA transcript in P. haemolytica A1 and the putative promoter of ssaI. The antigen (designated Ssa1) could be localized to the outer membrane of P. haemolytica A1 and E. coli clones carrying pSSA1. A rabbit serum against Ssa1 was produced by using whole cells of E. coli expressing Ssa1 on the surface as the immunogen, demonstrating that Ssa1 is immunogenic in rabbits. The results from colony immunoblot analysis with calf serum from animals that were resistant to P. haemolytica A1-induced pneumonia suggest indirectly that Ssa1 is also immunogenic in the animals.

This article has been cited by other articles:

• Gioia, J., Qin, X., Jiang, H., Clinkenbeard, K., Lo, R., Liu, Y., Fox, G. E., Yerrapragada, S., McLeod, M. P., McNeill, T. Z., Hemphill, L., Sodergren, E., Wang, Q., Muzny, D. M., Homsi, F. J., Weinstock, G. M., Highlander, S. K. (2006). The Genome Sequence of Mannheimia haemolytica A1: Insights into Virulence, Natural Competence, and Pasteurellaceae Phylogeny.. J. Bacteriol. 188: 7257-7266 [Abstract] [Full Text]  
• Henderson, I. R., Navarro-Garcia, F., Desvaux, M., Fernandez, R. C., Ala'Aldeen, D. (2004). Type V Protein Secretion Pathway: the Autotransporter Story. Microbiol. Mol. Biol. Rev. 68: 692-744 [Abstract] [Full Text]  
• Turner, D. P. J., Wooldridge, K. G., Ala'Aldeen, D. A. A. (2002). Autotransported Serine Protease A of Neisseria meningitidis: an Immunogenic, Surface-Exposed Outer Membrane, and Secreted Protein. Infect. Immun. 70: 4447-4461 [Abstract] [Full Text]  
• Fink, D. L., Cope, L. D., Hansen, E. J., Geme, J. W. St. III (2001). The Hemophilus influenzae Hap Autotransporter Is a Chymotrypsin Clan Serine Protease and Undergoes Autoproteolysis via an Intermolecular Mechanism. J. Biol. Chem. 276: 39492-39500 [Abstract] [Full Text]  
• Henderson, I. R., Nataro, J. P. (2001). Virulence Functions of Autotransporter Proteins. Infect. Immun. 69: 1231-1243 [Full Text]  
• Bohnsack, J. F., Takahashi, S., Hammitt, L., Miller, D. V., Aly, A. A., Adderson, E. E. (2000). Genetic Polymorphisms of Group B Streptococcus scpB Alter Functional Activity of a Cell-Associated Peptidase That Inactivates C5a. Infect. Immun. 68: 5018-5025 [Abstract] [Full Text]