This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kadurugamuwa, J L Articles by Timmis, K N Search for Related Content PubMed PubMed Citation Articles by Kadurugamuwa, J L Articles by Timmis, K N

 Previous Article  |  Next Article 

Infect Immun. 1991 October; 59(10): 3463-3471

Intercellular spread of Shigella flexneri through a monolayer mediated by membranous protrusions and associated with reorganization of the cytoskeletal protein vinculin.

Department of Microbiology, GBF-National Research Center for Biotechnology, Braunschweig, Germany.

ABSTRACT

The spread of Shigella flexneri in a monolayer of infected Henle and HeLa cells was studied by using immunofluorescence and electron microscopy. Infected cells produced numerous bacterium-containing membranous protrusions up to 18 microns in length that penetrated adjacent cells and were subsequently phagocytosed. Fluorescence staining of actin and vinculin in infected cells with phalloidin and monoclonal antibody to vinculin, respectively, demonstrated that the protrusions containing the bacteria consisted of these cytoskeletal proteins. Actin accumulated predominantly at the poles of bacteria distal to the tip of protrusions and appeared as trails extending back towards the host cell cytoplasm. Vinculin, however, was distributed uniformly around the bacteria and throughout the protrusion. A profound rearrangement of vinculin occurred in Henle and HeLa cells following infection with shigellae: whereas in uninfected cells it was distributed mainly around the cell periphery, in infected cells it concentrated mainly around clusters of bacteria in the cytoplasm. This suggests a possible involvement of the vinculin cytoskeletal protein in the intercellular spread of shigellae during an infection.

This article has been cited by other articles:

• Paetzold, S., Lourido, S., Raupach, B., Zychlinsky, A. (2007). Shigella flexneri Phagosomal Escape Is Independent of Invasion. Infect. Immun. 75: 4826-4830 [Abstract] [Full Text]  
• Miura, M., Terajima, J., Izumiya, H., Mitobe, J., Komano, T., Watanabe, H. (2006). OspE2 of Shigella sonnei Is Required for the Maintenance of Cell Architecture of Bacterium-Infected Cells.. Infect. Immun. 74: 2587-2595 [Abstract] [Full Text]  
• Burton, E. A., Oliver, T. N., Pendergast, A. M. (2005). Abl Kinases Regulate Actin Comet Tail Elongation via an N-WASP-Dependent Pathway. Mol. Cell. Biol. 25: 8834-8843 [Abstract] [Full Text]  
• Goldberg, M. B. (2001). Actin-Based Motility of Intracellular Microbial Pathogens. Microbiol. Mol. Biol. Rev. 65: 595-626 [Abstract] [Full Text]  
• Van Kirk, L. S., Hayes, S. F., Heinzen, R. A. (2000). Ultrastructure of Rickettsia rickettsii Actin Tails and Localization of Cytoskeletal Proteins. Infect. Immun. 68: 4706-4713 [Abstract] [Full Text]  
• Rathman, M, de Lanerolle, P, Ohayon, H, Gounon, P, Sansonetti, P (2000). Myosin light chain kinase plays an essential role in S. flexneri dissemination. J. Cell Sci. 113: 3375-3386 [Abstract]  
• Meyer, D. H., Rose, J. E., Lippmann, J. E., Fives-Taylor, P. M. (1999). Microtubules Are Associated with Intracellular Movement and Spread of the Periodontopathogen Actinobacillus actinomycetemcomitans. Infect. Immun. 67: 6518-6525 [Abstract] [Full Text]  
• Suzuki, T., Mimuro, H., Miki, H., Takenawa, T., Sasaki, T., Nakanishi, H., Takai, Y., Sasakawa, C. (1999). Rho Family GTPase Cdc42 Is Essential for the Actin-based Motility of Shigella in Mammalian Cells. J. Exp. Med. 191: 1905-1920 [Abstract] [Full Text]  
• Charles, M., Magdalena, J., Theriot, J. A., Goldberg, M. B. (1999). Functional Analysis of a Rickettsial OmpA Homology Domain of Shigella flexneri IcsA. J. Bacteriol. 181: 869-878 [Abstract] [Full Text]  
• Gouin, E, Gantelet, H, Egile, C, Lasa, I, Ohayon, H, Villiers, V, Gounon, P, Sansonetti, P., Cossart, P (1999). A comparative study of the actin-based motilities of the pathogenic bacteria Listeria monocytogenes, Shigella flexneri and Rickettsia conorii. J. Cell Sci. 112: 1697-1708 [Abstract]  
• Kadurugamuwa, J. L., Beveridge, T. J. (1998). Delivery of the Non-Membrane-Permeative Antibiotic Gentamicin into Mammalian Cells by Using Shigella flexneri Membrane Vesicles. Antimicrob. Agents Chemother. 42: 1476-1483 [Abstract] [Full Text]  
• Laine, R. O., Zeile, W., Kang, F., Purich, D. L., Southwick, F. S. (1997). Vinculin Proteolysis Unmasks an ActA Homolog for Actin-based Shigella Motility. J. Cell Biol. 138: 1255-1264 [Abstract] [Full Text]  
• Suzuki, T., Saga, S., Sasakawa, C. (1996). Functional Analysis of Shigella VirG Domains Essential for Interaction with Vinculin and Actin-based Motility. J. Biol. Chem. 271: 21878-21885 [Abstract] [Full Text]  
• Mimuro, H., Suzuki, T., Suetsugu, S., Miki, H., Takenawa, T., Sasakawa, C. (2000). Profilin Is Required for Sustaining Efficient Intra- and Intercellular Spreading of Shigella flexneri. J. Biol. Chem. 275: 28893-28901 [Abstract] [Full Text]