This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arakere, G Articles by Frasch, C E Search for Related Content PubMed PubMed Citation Articles by Arakere, G Articles by Frasch, C E

 Previous Article  |  Next Article 

Infect Immun. 1991 December; 59(12): 4349-4356

Specificity of antibodies to O-acetyl-positive and O-acetyl-negative group C meningococcal polysaccharides in sera from vaccinees and carriers.

Division of Bacterial Products, Food and Drug Administration, Bethesda, Maryland 20892.

ABSTRACT

Most group C Neisseria meningitidis strains produce an O-acetyl-positive polysaccharide, a homopolymer of alpha-2----9-linked N-acetylneuraminic acid with O-acetyl groups at the C-7 and C-8 of its sialic acid residues. The majority of disease isolates have been reported to contain this polysaccharide. Some strains produce group C polysaccharide lacking O-acetyl groups. The licensed vaccine contains the O-acetyl-positive polysaccharide. We have measured the antibody specificities to the two polysaccharides in sera from asymptomatic group C meningococcal carriers and vaccinated adults by a new enzyme-linked immunosorbent assay (ELISA) procedure using methylated human serum albumin for coating the group C polysaccharide onto microtiter plates. Inhibition of binding of serum antibodies to polysaccharide-coated plates was measured by ELISA after incubation with O-acetyl-positive and O-acetyl-negative group C polysaccharides. Greater inhibition of binding of carrier sera was observed with the homologous polysaccharide. There was substantial inhibition of binding of vaccinee sera to the O-acetyl-positive polysaccharide-coated plate following preincubation with O-acetyl-positive polysaccharide, but homologous inhibition on plates coated with the O-acetyl-negative polysaccharide required much higher concentrations of polysaccharide. Carrier sera may have a higher proportion of antibodies with greater specificity for the O-acetyl-negative polysaccharide, while vaccinee sera contain antibodies with greater affinity for the O-acetyl-positive polysaccharide. Studies with monoclonal antibodies specific for O-acetyl-positive and O-acetyl-negative polysaccharides reveal that the percentage of group C meningococcal disease caused by O-acetyl-negative strains remains about 15%, as found over 15 years ago.

This article has been cited by other articles:

• Toropainen, M., Saarinen, L., Wedege, E., Bolstad, K., Michaelsen, T. E., Aase, A., Kayhty, H. (2005). Protection by Natural Human Immunoglobulin M Antibody to Meningococcal Serogroup B Capsular Polysaccharide in the Infant Rat Protection Assay Is Independent of Complement-Mediated Bacterial Lysis. Infect. Immun. 73: 4694-4703 [Abstract] [Full Text]  
• Giardina, P. C., Longworth, E., Evans-Johnson, R. E., Bessette, M. L., Zhang, H., Borrow, R., Madore, D., Fernsten, P. (2005). Analysis of Human Serum Immunoglobulin G against O-Acetyl-Positive and O-Acetyl-Negative Serogroup W135 Meningococcal Capsular Polysaccharide. CVI 12: 586-592 [Abstract] [Full Text]  
• Lewis, A. L., Nizet, V., Varki, A. (2004). Discovery and characterization of sialic acid O-acetylation in group B Streptococcus. Proc. Natl. Acad. Sci. USA 101: 11123-11128 [Abstract] [Full Text]  
• Garcia-Ojeda, P. A., Hardy, S., Kozlowski, S., Stein, K. E., Feavers, I. M. (2004). Surface Plasmon Resonance Analysis of Antipolysaccharide Antibody Specificity: Responses to Meningococcal Group C Conjugate Vaccines and Bacteria. Infect. Immun. 72: 3451-3460 [Abstract] [Full Text]  
• Giardina, P. C., Evans, R. E., Sikkema, D. J., Madore, D., Hildreth, S. W. (2003). Effect of Antigen Coating Conditions on Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G Antibody to Neisseria meningitidis Serogroup Y and W135 Capsular Polysaccharide Antigens in Serum. CVI 10: 1136-1140 [Abstract] [Full Text]  
• Harris, S. L., King, W. J., Ferris, W., Granoff, D. M. (2003). Age-Related Disparity in Functional Activities of Human Group C Serum Anticapsular Antibodies Elicited by Meningococcal Polysaccharide Vaccine. Infect. Immun. 71: 275-286 [Abstract] [Full Text]  
• Berry, D. S., Lynn, F., Lee, C.-H., Frasch, C. E., Bash, M. C. (2002). Effect of O Acetylation of Neisseria meningitidis Serogroup A Capsular Polysaccharide on Development of Functional Immune Responses. Infect. Immun. 70: 3707-3713 [Abstract] [Full Text]  
• Guttormsen, H.-K., Baker, C. J., Nahm, M. H., Paoletti, L. C., Zughaier, S. M., Edwards, M. S., Kasper, D. L. (2002). Type III Group B Streptococcal Polysaccharide Induces Antibodies That Cross-React with Streptococcus pneumoniae Type 14. Infect. Immun. 70: 1724-1738 [Abstract] [Full Text]  
• Coquillat, D., Bruge, J., Danve, B., Latour, M., Hurpin, C., Schulz, D., Durbec, P., Rougon, G. (2001). Activity and Cross-Reactivity of Antibodies Induced in Mice by Immunization with a Group B Meningococcal Conjugate. Infect. Immun. 69: 7130-7139 [Abstract] [Full Text]  
• Richmond, P., Borrow, R., Findlow, J., Martin, S., Thornton, C., Cartwright, K., Miller, E. (2001). Evaluation of De-O-Acetylated Meningococcal C Polysaccharide-Tetanus Toxoid Conjugate Vaccine in Infancy: Reactogenicity, Immunogenicity, Immunologic Priming, and Bactericidal Activity against O-Acetylated and De-O-Acetylated Serogroup C Strains. Infect. Immun. 69: 2378-2382 [Abstract] [Full Text]  
• Fattom, A. I., Sarwar, J., Basham, L., Ennifar, S., Naso, R. (1998). Antigenic Determinants of Staphylococcus aureus Type 5 and Type 8 Capsular Polysaccharide Vaccines. Infect. Immun. 66: 4588-4592 [Abstract] [Full Text]  
• Concepcion, N., Frasch, C. E. (1998). Evaluation of Previously Assigned Antibody Concentrations in Pneumococcal Polysaccharide Reference Serum 89SF by the Method of Cross-Standardization. CVI 5: 199-204 [Abstract] [Full Text]