This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schmitt, M P Articles by Holmes, R K Search for Related Content PubMed PubMed Citation Articles by Schmitt, M P Articles by Holmes, R K

 Previous Article  |  Next Article 

Infect Immun. 1991 June; 59(6): 1899-1904

Iron-dependent regulation of diphtheria toxin and siderophore expression by the cloned Corynebacterium diphtheriae repressor gene dtxR in C. diphtheriae C7 strains.

Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.

ABSTRACT

A regulatory gene (dtxR) responsible for iron-dependent repression of the toxin (tox) and siderophore genes in Corynebacterium diphtheriae was cloned and characterized. A DNA fragment carrying dtxR repressed expression of a tox-lacZ gene fusion in Escherichia coli DH5 alpha in a high-iron environment but not under low-iron conditions. A protein with mobility corresponding to approximately 28 to 29 kDa was identified as the product of the dtxR gene by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A shuttle vector designated pCM2.6 was constructed which carries the origin of replication from C. diphtheriae plasmid pNG2 and confers resistance to chloramphenicol in E. coli and C. diphtheriae. DNA fragments carrying dtxR were cloned into pCM2.6, and the hybrid shuttle plasmids were transformed by electroporation into wild-type C. diphtheriae C7(beta) and the regulatory mutant C7(beta)hm723, which produces toxin and siderophore constitutively under high-iron conditions. Expression of the cloned dtxR determinant did not affect the phenotype of C. diphtheriae C7(beta). In C. diphtheriae C7(beta)hm723, expression of cloned dtxR restored full repression of siderophore production and partial repression of diphtheria toxin production during growth in a high-iron environment.

This article has been cited by other articles:

• Frunzke, J., Bramkamp, M., Schweitzer, J.-E., Bott, M. (2008). Population Heterogeneity in Corynebacterium glutamicum ATCC 13032 Caused by Prophage CGP3. J. Bacteriol. 190: 5111-5119 [Abstract] [Full Text]  
• Miranda-CasoLuengo, R., Prescott, J. F., Vazquez-Boland, J. A., Meijer, W. G. (2008). The Intracellular Pathogen Rhodococcus equi Produces a Catecholate Siderophore Required for Saprophytic Growth. J. Bacteriol. 190: 1631-1637 [Abstract] [Full Text]  
• Murphy, E. R., Payne, S. M. (2007). RyhB, an Iron-Responsive Small RNA Molecule, Regulates Shigella dysenteriae Virulence. Infect. Immun. 75: 3470-3477 [Abstract] [Full Text]  
• Kunkle, C. A., Schmitt, M. P. (2007). Comparative Analysis of hmuO Function and Expression in Corynebacterium Species. J. Bacteriol. 189: 3650-3654 [Abstract] [Full Text]  
• Bibb, L. A., Kunkle, C. A., Schmitt, M. P. (2007). The ChrA-ChrS and HrrA-HrrS Signal Transduction Systems Are Required for Activation of the hmuO Promoter and Repression of the hemA Promoter in Corynebacterium diphtheriae. Infect. Immun. 75: 2421-2431 [Abstract] [Full Text]  
• Wennerhold, J., Bott, M. (2006). The DtxR Regulon of Corynebacterium glutamicum.. J. Bacteriol. 188: 2907-2918 [Abstract] [Full Text]  
• Oram, D. M., Jacobson, A. D., Holmes, R. K. (2006). Transcription of the Contiguous sigB, dtxR, and galE Genes in Corynebacterium diphtheriae: Evidence for Multiple Transcripts and Regulation by Environmental Factors.. J. Bacteriol. 188: 2959-2973 [Abstract] [Full Text]  
• Johnston, J. W., Briles, D. E., Myers, L. E., Hollingshead, S. K. (2006). Mn2+-Dependent Regulation of Multiple Genes in Streptococcus pneumoniae through PsaR and the Resultant Impact on Virulence. Infect. Immun. 74: 1171-1180 [Abstract] [Full Text]  
• Bibb, L. A., King, N. D., Kunkle, C. A., Schmitt, M. P. (2005). Analysis of a Heme-Dependent Signal Transduction System in Corynebacterium diphtheriae: Deletion of the chrAS Genes Results in Heme Sensitivity and Diminished Heme-Dependent Activation of the hmuO Promoter. Infect. Immun. 73: 7406-7412 [Abstract] [Full Text]  
• Bates, C. S., Toukoki, C., Neely, M. N., Eichenbaum, Z. (2005). Characterization of MtsR, a New Metal Regulator in Group A Streptococcus, Involved in Iron Acquisition and Virulence. Infect. Immun. 73: 5743-5753 [Abstract] [Full Text]  
• Miranda-CasoLuengo, R., Duffy, P. S., O'Connell, E. P., Graham, B. J., Mangan, M. W., Prescott, J. F., Meijer, W. G. (2005). The Iron-Regulated iupABC Operon Is Required for Saprophytic Growth of the Intracellular Pathogen Rhodococcus equi at Low Iron Concentrations. J. Bacteriol. 187: 3438-3444 [Abstract] [Full Text]  
• Prakash, P., Yellaboina, S., Ranjan, A., Hasnain, S. E. (2005). Computational prediction and experimental verification of novel IdeR binding sites in the upstream sequences of Mycobacterium tuberculosis open reading frames. Bioinformatics 21: 2161-2166 [Abstract] [Full Text]  
• Kunkle, C. A., Schmitt, M. P. (2005). Analysis of a DtxR-Regulated Iron Transport and Siderophore Biosynthesis Gene Cluster in Corynebacterium diphtheriae. J. Bacteriol. 187: 422-433 [Abstract] [Full Text]  
• De Zoysa, A., Efstratiou, A., Hawkey, P. M. (2005). Molecular Characterization of Diphtheria Toxin Repressor (dtxR) Genes Present in Nontoxigenic Corynebacterium diphtheriae Strains Isolated in the United Kingdom. J. Clin. Microbiol. 43: 223-228 [Abstract] [Full Text]  
• Oram, D. M., Avdalovic, A., Holmes, R. K. (2004). Analysis of Genes That Encode DtxR-Like Transcriptional Regulators in Pathogenic and Saprophytic Corynebacterial Species. Infect. Immun. 72: 1885-1895 [Abstract] [Full Text]  
• Love, J. F., vanderSpek, J. C., Marin, V., Guerrero, L., Logan, T. M., Murphy, J. R. (2004). Genetic and biophysical studies of diphtheria toxin repressor (DtxR) and the hyperactive mutant DtxR(E175K) support a multistep model of activation. Proc. Natl. Acad. Sci. USA 101: 2506-2511 [Abstract] [Full Text]  
• Kunkle, C. A., Schmitt, M. P. (2003). Analysis of the Corynebacterium diphtheriae DtxR Regulon: Identification of a Putative Siderophore Synthesis and Transport System That Is Similar to the Yersinia High-Pathogenicity Island-Encoded Yersiniabactin Synthesis and Uptake System. J. Bacteriol. 185: 6826-6840 [Abstract] [Full Text]  
• Sheehan, B. J., Bosse, J. T., Beddek, A. J., Rycroft, A. N., Kroll, J. S., Langford, P. R. (2003). Identification of Actinobacillus pleuropneumoniae Genes Important for Survival during Infection in Its Natural Host. Infect. Immun. 71: 3960-3970 [Abstract] [Full Text]  
• Ando, M., Manabe, Y. C., Converse, P. J., Miyazaki, E., Harrison, R., Murphy, J. R., Bishai, W. R. (2003). Characterization of the Role of the Divalent Metal Ion-Dependent Transcriptional Repressor MntR in the Virulence of Staphylococcus aureus. Infect. Immun. 71: 2584-2590 [Abstract] [Full Text]  
• Spiering, M. M., Ringe, D., Murphy, J. R., Marletta, M. A. (2003). Bioinorganic Chemistry Special Feature: Metal stoichiometry and functional studies of the diphtheria toxin repressor. Proc. Natl. Acad. Sci. USA 100: 3808-3813 [Abstract] [Full Text]  
• Schmitt, M. P. (2002). Analysis of a DtxR-Like Metalloregulatory Protein, MntR, from Corynebacterium diphtheriae That Controls Expression of an ABC Metal Transporter by an Mn2+-Dependent Mechanism. J. Bacteriol. 184: 6882-6892 [Abstract] [Full Text]  
• Oram, D. M., Avdalovic, A., Holmes, R. K. (2002). Construction and Characterization of Transposon Insertion Mutations in Corynebacterium diphtheriae That Affect Expression of the Diphtheria Toxin Repressor (DtxR). J. Bacteriol. 184: 5723-5732 [Abstract] [Full Text]  
• Qian, Y., Lee, J. H., Holmes, R. K. (2002). Identification of a DtxR-Regulated Operon That Is Essential for Siderophore-Dependent Iron Uptake in Corynebacterium diphtheriae. J. Bacteriol. 184: 4846-4856 [Abstract] [Full Text]  
• Wagner, P. L., Waldor, M. K. (2002). Bacteriophage Control of Bacterial Virulence. Infect. Immun. 70: 3985-3993 [Full Text]  
• Kirby, A. E., Metzger, D. J., Murphy, E. R., Connell, T. D. (2001). Heme Utilization in Bordetella avium Is Regulated by RhuI, a Heme-Responsive Extracytoplasmic Function Sigma Factor. Infect. Immun. 69: 6951-6961 [Abstract] [Full Text]  
• Twigg, P. D., Parthasarathy, G., Guerrero, L., Logan, T. M., Caspar, D. L. D. (2001). Disordered to ordered folding in the regulation of diphtheria toxin repressor activity. Proc. Natl. Acad. Sci. USA 98: 11259-11264 [Abstract] [Full Text]  
• Schmitt, M. P., Drazek, E. S. (2001). Construction and Consequences of Directed Mutations Affecting the Hemin Receptor in Pathogenic Corynebacterium Species. J. Bacteriol. 183: 1476-1481 [Abstract] [Full Text]  
• Jarosik, G. P., Land, C. B. (2000). Identification of a Human Lactoferrin-Binding Protein in Gardnerella vaginalis. Infect. Immun. 68: 3443-3447 [Abstract] [Full Text]  
• Wilks, A., Moenne-Loccoz, P. (2000). Identification of the Proximal Ligand His-20 in Heme Oxygenase (Hmu O) from Corynebacterium diphtheriae. OXIDATIVE CLEAVAGE OF THE HEME MACROCYCLE DOES NOT REQUIRE THE PROXIMAL HISTIDINE. J. Biol. Chem. 275: 11686-11692 [Abstract] [Full Text]  
• Lee, J. H., Holmes, R. K. (2000). Characterization of Specific Nucleotide Substitutions in DtxR-Specific Operators of Corynebacterium diphtheriae That Dramatically Affect DtxR Binding, Operator Function, and Promoter Strength. J. Bacteriol. 182: 432-438 [Abstract] [Full Text]  
• Schmitt, M. P. (1999). Identification of a Two-Component Signal Transduction System from Corynebacterium diphtheriae That Activates Gene Expression in Response to the Presence of Heme and Hemoglobin. J. Bacteriol. 181: 5330-5340 [Abstract] [Full Text]  
• Murphy, E. R., Dickenson, A., Militello, K. T., Connell, T. D. (1999). Genetic Characterization of Wild-Type and Mutant fur Genes of Bordetella avium. Infect. Immun. 67: 3160-3165 [Abstract] [Full Text]  
• Goranson-Siekierke, J., Pohl, E., Hol, W. G. J., Holmes, R. K. (1999). Anion-Coordinating Residues at Binding Site 1 Are Essential for the Biological Activity of the Diphtheria Toxin Repressor. Infect. Immun. 67: 1806-1811 [Abstract] [Full Text]  
• Sun, L., vanderSpek, J., Murphy, J. R. (1998). Isolation and characterization of iron-independent positive dominant mutants of the diphtheria toxin repressor DtxR. Proc. Natl. Acad. Sci. USA 95: 14985-14990 [Abstract] [Full Text]  
• Jarosik, G. P., Land, C. B., Duhon, P., Chandler, R. Jr., Mercer, T. (1998). Acquisition of Iron by Gardnerella vaginalis. Infect. Immun. 66: 5041-5047 [Abstract] [Full Text]  
• Pohl, E., Holmes, R. K., Hol, W. G. J. (1998). Motion of the DNA-binding Domain with Respect to the Core of the Diphtheria Toxin Repressor (DtxR) Revealed in the Crystal Structures of Apo- and Holo-DtxR. J. Biol. Chem. 273: 22420-22427 [Abstract] [Full Text]  
• Connell, T. D., Dickenson, A., Martone, A. J., Militello, K. T., Filiatraut, M. J., Hayman, M. L., Pitula, J. (1998). Iron Starvation of Bordetella avium Stimulates Expression of Five Outer Membrane Proteins and Regulates a Gene Involved in Acquiring Iron from Serum. Infect. Immun. 66: 3597-3605 [Abstract] [Full Text]  
• Wilks, A., Schmitt, M. P. (1998). Expression and Characterization of a Heme Oxygenase (Hmu O) from Corynebacterium diphtheriae. IRON ACQUISITION REQUIRES OXIDATIVE CLEAVAGE OF THE HEME MACROCYCLE. J. Biol. Chem. 273: 837-841 [Abstract] [Full Text]  
• O'Halloran, T. (1993). Transition metals in control of gene expression. Science 261: 715-725 [Abstract]  
• Feese, M. D., Ingason, B. P., Goranson-Siekierke, J., Holmes, R. K., Hol, W. G. J. (2001). Crystal Structure of the Iron-dependent Regulator from Mycobacterium tuberculosis at 2.0-A Resolution Reveals the Src Homology Domain 3-like Fold and Metal Binding Function of the Third Domain. J. Biol. Chem. 276: 5959-5966 [Abstract] [Full Text]