This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sturman, L. S. Articles by Takemoto, K. K. Search for Related Content PubMed PubMed Citation Articles by Sturman, L. S. Articles by Takemoto, K. K.

Enhanced Growth of a Murine Coronavirus in Transformed Mouse Cells

¹ Virus Laboratory, Division of Laboratories and Research, New York State Department of Health, Albany, New York 12201
² Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014

ABSTRACT

Plaque formation by A59 virus, a murine coronavirus, was facilitated in AL/N and Balb mouse cells transformed by polyoma virus, simian virus 40, murine sarcoma virus, or mammary tumor virus. In these virus-transformed cells, A59 virus plaques were larger, they appeared earlier, and plaquing efficiencies were higher than in normal, untransformed cells. "Spontaneously" transformed AL/N cells behaved similarly to untransformed cells, whereas "spontaneously" transformed Balb cells resembled virus-transformed cell hosts. Both untransformed and transformed AL/N and Balb cells were permissive hosts for A59 virus. However, multiplication of A59 virus was enhanced at least fivefold in the virus-transformed AL/N cell hosts. Larger differences (100-fold or greater) in A59 virus production were obtained during the first cycle of infection in Balb cells at low multiplicities and in AL/N cells after multiple cycles of virus growth. In virus-transformed and "spontaneously" transformed Balb cells, A59 virus induced extensive syncytia formation.

This article has been cited by other articles:

• Bechill, J., Chen, Z., Brewer, J. W., Baker, S. C. (2008). Coronavirus Infection Modulates the Unfolded Protein Response and Mediates Sustained Translational Repression. J. Virol. 82: 4492-4501 [Abstract] [Full Text]  
• Slobodskaya, O., Laarman, A., Spaan, W. J. M. (2008). Intracellular Restriction of a Productive Noncytopathic Coronavirus Infection. J. Virol. 82: 451-460 [Abstract] [Full Text]  
• Eifart, P., Ludwig, K., Bottcher, C., de Haan, C. A. M., Rottier, P. J. M., Korte, T., Herrmann, A. (2007). Role of Endocytosis and Low pH in Murine Hepatitis Virus Strain A59 Cell Entry. J. Virol. 81: 10758-10768 [Abstract] [Full Text]  
• Ye, Y., Hauns, K., Langland, J. O., Jacobs, B. L., Hogue, B. G. (2007). Mouse Hepatitis Coronavirus A59 Nucleocapsid Protein Is a Type I Interferon Antagonist. J. Virol. 81: 2554-2563 [Abstract] [Full Text]  
• De Albuquerque, N., Baig, E., Ma, X., Zhang, J., He, W., Rowe, A., Habal, M., Liu, M., Shalev, I., Downey, G. P., Gorczynski, R., Butany, J., Leibowitz, J., Weiss, S. R., McGilvray, I. D., Phillips, M. J., Fish, E. N., Levy, G. A. (2006). Murine hepatitis virus strain 1 produces a clinically relevant model of severe acute respiratory syndrome in a/j mice.. J. Virol. 80: 10382-10394 [Abstract] [Full Text]  
• Versteeg, G. A., Slobodskaya, O., Spaan, W. J. M. (2006). Transcriptional profiling of acute cytopathic murine hepatitis virus infection in fibroblast-like cells. J. Gen. Virol. 87: 1961-1975 [Abstract] [Full Text]  
• Thorp, E. B., Boscarino, J. A., Logan, H. L., Goletz, J. T., Gallagher, T. M. (2006). Palmitoylations on Murine Coronavirus Spike Proteins Are Essential for Virion Assembly and Infectivity. J. Virol. 80: 1280-1289 [Abstract] [Full Text]  
• Coley, S. E., Lavi, E., Sawicki, S. G., Fu, L., Schelle, B., Karl, N., Siddell, S. G., Thiel, V. (2005). Recombinant Mouse Hepatitis Virus Strain A59 from Cloned, Full-Length cDNA Replicates to High Titers In Vitro and Is Fully Pathogenic In Vivo. J. Virol. 79: 3097-3106 [Abstract] [Full Text]  
• Chen, C.-J., Sugiyama, K., Kubo, H., Huang, C., Makino, S. (2004). Murine Coronavirus Nonstructural Protein p28 Arrests Cell Cycle in G0/G1 Phase. J. Virol. 78: 10410-10419 [Abstract] [Full Text]  
• Chen, C.-J., Makino, S. (2004). Murine Coronavirus Replication Induces Cell Cycle Arrest in G0/G1 Phase. J. Virol. 78: 5658-5669 [Abstract] [Full Text]  
• Thorp, E. B., Gallagher, T. M. (2004). Requirements for CEACAMs and Cholesterol during Murine Coronavirus Cell Entry. J. Virol. 78: 2682-2692 [Abstract] [Full Text]  
• Ng, M.-L., Tan, S.-H., See, E.-E., Ooi, E.-E., Ling, A.-E. (2003). Proliferative growth of SARS coronavirus in Vero E6 cells. J. Gen. Virol. 84: 3291-3303 [Abstract] [Full Text]  
• Banerjee, S., Narayanan, K., Mizutani, T., Makino, S. (2002). Murine Coronavirus Replication-Induced p38 Mitogen-Activated Protein Kinase Activation Promotes Interleukin-6 Production and Virus Replication in Cultured Cells. J. Virol. 76: 5937-5948 [Abstract] [Full Text]  
• Gosert, R., Kanjanahaluethai, A., Egger, D., Bienz, K., Baker, S. C. (2002). RNA Replication of Mouse Hepatitis Virus Takes Place at Double-Membrane Vesicles. J. Virol. 76: 3697-3708 [Abstract] [Full Text]  
• Sawicki, D. L., Wang, T., Sawicki, S. G. (2001). The RNA structures engaged in replication and transcription of the A59 strain of mouse hepatitis virus. J. Gen. Virol. 82: 385-396 [Abstract] [Full Text]  
• Kanjanahaluethai, A., Baker, S. C. (2000). Identification of Mouse Hepatitis Virus Papain-Like Proteinase 2 Activity. J. Virol. 74: 7911-7921 [Abstract] [Full Text]  
• An, S., Chen, C.-J., Yu, X., Leibowitz, J. L., Makino, S. (1999). Induction of Apoptosis in Murine Coronavirus-Infected Cultured Cells and Demonstration of E Protein as an Apoptosis Inducer. J. Virol. 73: 7853-7859 [Abstract] [Full Text]  
• Shi, S. T., Schiller, J. J., Kanjanahaluethai, A., Baker, S. C., Oh, J.-W., Lai, M. M. C. (1999). Colocalization and Membrane Association of Murine Hepatitis Virus Gene 1 Products and De Novo-Synthesized Viral RNA in Infected Cells. J. Virol. 73: 5957-5969 [Abstract] [Full Text]  
• Pope, M., Marsden, P. A., Cole, E., Sloan, S., Fung, L. S., Ning, Q., Ding, J. W., Leibowitz, J. L., Phillips, M. J., Levy, G. A. (1998). Resistance to Murine Hepatitis Virus Strain 3 Is Dependent on Production of Nitric Oxide. J. Virol. 72: 7084-7090 [Abstract] [Full Text]  
• Rao, P. V., Gallagher, T. M. (1998). Intracellular Complexes of Viral Spike and Cellular Receptor Accumulate during Cytopathic Murine Coronavirus Infections. J. Virol. 72: 3278-3288 [Abstract] [Full Text]