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Cells Producing Low-Molecular-Weight Antibody to Escherichia coli Lipopolysaccharide

^a Department of Microbiology, Georgetown University Schools of Medicine and Dentistry, Washington, D.C. 20007

ABSTRACT

CD-1 mice immunized with sheep red blood cells (SRBC) or Escherichia coli lipopolysaccharide (LPS), developed splenic plaque-forming cells (PFC) producing low-molecular-weight antibody; these cells were detected by means of purified rabbit antisera to mouse ₁, _2a, and _2b immunoglobulins. In contrast to SRBC, the primary ₁ response to LPS was absent and _2a and _2b PFC were detected irregularly. Both immunogens elicited a secondary cellular response in all three subclasses without a corresponding increase in "direct" or _M PFC. An increase in serum bactericidal antibody following a second injection of LPS was not parallelled by an increase in splenic _M PFC; it might therefore involve the synthesis of ₂ complement-fixing antibody.

¹ Present address: Meloy Laboratories, Biological Products Division, Springfield, Va. 22151.

² Present address: Department of Microbiology, University of Colorado Medical Center, Denver, Colo. 80220.

³ In partial fulfillment of the requirements for the Doctor of Philosophy Degree, Georgetown University.