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Titration of Antibodies to Salmonella O Antigens by Enzyme-Linked Immunosorbent Assay

Department of Immunology, Wenner Gren Institute, University of Stockholm, S-113 45 Stockholm, Sweden
Department of Bacteriology, National Bacteriological Laboratory, S-105 21 Stockholm, Sweden

ABSTRACT

A new type of immunoassay (enzyme-linked immunosorbent assay, ELISA) has been used for the detection of antibodies against Salmonella O antigens. The results show that ELISA is specific and highly sensitive. When compared with the Widal reaction, passive hemagglutination, and quantitative precipitation, ELISA was shown to be more sensitive. In addition, immunoglobulin G and immunoglobulin M antibodies were both detected approximately as efficiently with ELISA.

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