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Infect Immun. 1972 November; 6(5): 793-797
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Abortive Infection of Human Diploid Cells by Murine Cytomegalovirus

¹ Department of Microbiology and Animal Experimentation, New York State Institute for Research in Mental Retardation, Staten Island, New York 10314

ABSTRACT

Inoculation of human diploid cells (WI-38) with murine cytomegalovirus (MCMV) did not result in the synthesis of any infectious virions. However, morphological changes typical of the cytopathic effects (CPE) of MCMV were detectable within 12 hr of infection. The CPE included rounding, swelling, and detachment of cells. The nuclei of infected cells were enlarged, and intranuclear inclusions were visible by May Grunwald-Giemsa staining and by the indirect fluorescent-antibody test. All cells infected at a multiplicity of infection of 3 showed CPE, and these cells could not be passaged successfully. Cell lysates and exhausted media from infected WI-38 cultures did not produce any CPE in WI-38 cells. The virus absorbed to WI-38 cells with the same efficiency as to mouse embryo fibroblast cells (MEF). Samples of MCMV in which virus infectivity for MEF cells had been inactivated by ultraviolet irradiation or by exposure to 56 C failed to produce any of the above signs. MCMV-specific CPE did not occur in the presence of actinomycin D (1 µg/ml) or puromycin (20 µg/ml), but 5'-fluoro-2'-deoxyuridine at 1 x 10^–4M did not prevent CPE or the development of intranuclear inclusions.

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