This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maurin, M
Right arrow Articles by Raoult, D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maurin, M
Right arrow Articles by Raoult, D

 Previous Article  |  Next Article 

Infect Immun. 1992 December; 60(12): 5013-5016

Phagolysosomes of Coxiella burnetii-infected cell lines maintain an acidic pH during persistent infection.

M Maurin, A M Benoliel, P Bongrand and D Raoult

Centre National de Référence des Rickettsioses, CHU La Timone, Marseille.

ABSTRACT

Coxiella burnetii, the agent of Q fever, is an obligate intracellular bacterium that multiples within vacuoles of phagolysosomal origin. Persistently infected cell lines were maintained in continuous culture for months. We studied the pH of the phagolysosomes by using two murine cell lines during early propagation of the bacteria and after establishment of persistent infection. Three strains of C. burnetii were studied because of the purported propensity of each strain to cause acute or chronic disease and to be resistant or susceptible to antibiotics. The pHs were calculated from fluorescence experiments with fluoresceinated dextran as a lysosomal probe. Phagolysosomal vacuoles maintained an acidic pH during a 36-day infection. Minimal variation of the pH occurred over the duration of the experiment with strains that caused either acute or chronic disease. Phagolysosomal pH remained stable for as long as 153 days with the Nine Mile phase II isolate. Thus, neither the course of C. burnetti infection nor the diversity of antibiotic susceptibility of the strains is related to variations in the phagolysosomal pH.

Infect Immun. 1992 December; 60(12): 5013-5016




This article has been cited by other articles:

  • Marmion, B.P., Sukocheva, O., Storm, P.A., Lockhart, M., Turra, M., Kok, T., Ayres, J., Routledge, H., Graves, S. (2009). Q fever: persistence of antigenic non-viable cell residues of Coxiella burnetii in the host--implications for post Q fever infection fatigue syndrome and other chronic sequelae. QJM 102: 673-684 [Abstract] [Full Text]  
  • Vandal, O. H., Nathan, C. F., Ehrt, S. (2009). Acid Resistance in Mycobacterium tuberculosis. J. Bacteriol. 191: 4714-4721 [Full Text]  
  • Raghavan, R., Hicks, L. D., Minnick, M. F. (2008). Toxic Introns and Parasitic Intein in Coxiella burnetii: Legacies of a Promiscuous Past. J. Bacteriol. 190: 5934-5943 [Abstract] [Full Text]  
  • Omsland, A., Cockrell, D. C., Fischer, E. R., Heinzen, R. A. (2008). Sustained Axenic Metabolic Activity by the Obligate Intracellular Bacterium Coxiella burnetii. J. Bacteriol. 190: 3203-3212 [Abstract] [Full Text]  
  • Luhrmann, A., Roy, C. R. (2007). Coxiella burnetii Inhibits Activation of Host Cell Apoptosis through a Mechanism That Involves Preventing Cytochrome c Release from Mitochondria. Infect. Immun. 75: 5282-5289 [Abstract] [Full Text]  
  • Chu, H., Lee, J.-H., Han, S.-H., Kim, S.-Y., Cho, N.-H., Kim, I.-S., Choi, M.-S. (2006). Exploitation of the Endocytic Pathway by Orientia tsutsugamushi in Nonprofessional Phagocytes. Infect. Immun. 74: 4246-4253 [Abstract] [Full Text]  
  • Batista, D. G.J., Silva, C. F., Mota, R. A., Costa, L. C., Meirelles, M. N.L., Meuser-Batista, M., Soeiro, M. N.C. (2006). Trypanosoma cruzi Modulates the Expression of Rabs and Alters the Endocytosis in Mouse Cardiomyocytes In Vitro. J. Histochem. Cytochem. 54: 605-614 [Abstract] [Full Text]  
  • Newman, S. L., Gootee, L., Hilty, J., Morris, R. E. (2006). Human Macrophages Do Not Require Phagosome Acidification to Mediate Fungistatic/Fungicidal Activity against Histoplasma capsulatum. J. Immunol. 176: 1806-1813 [Abstract] [Full Text]  
  • Zamboni, D. S., Campos, M. A., Torrecilhas, A. C. T., Kiss, K., Samuel, J. E., Golenbock, D. T., Lauw, F. N., Roy, C. R., Almeida, I. C., Gazzinelli, R. T. (2004). Stimulation of Toll-like Receptor 2 by Coxiella burnetii Is Required for Macrophage Production of Pro-inflammatory Cytokines and Resistance to Infection. J. Biol. Chem. 279: 54405-54415 [Abstract] [Full Text]  
  • Coleman, S. A., Fischer, E. R., Howe, D., Mead, D. J., Heinzen, R. A. (2004). Temporal Analysis of Coxiella burnetii Morphological Differentiation. J. Bacteriol. 186: 7344-7352 [Abstract] [Full Text]  
  • Drevets, D. A., Leenen, P. J. M., Greenfield, R. A. (2004). Invasion of the Central Nervous System by Intracellular Bacteria. Clin. Microbiol. Rev. 17: 323-347 [Abstract] [Full Text]  
  • Greub, G., Raoult, D. (2004). Microorganisms Resistant to Free-Living Amoebae. Clin. Microbiol. Rev. 17: 413-433 [Abstract] [Full Text]  
  • Zamboni, D. S. (2004). Genetic Control of Natural Resistance of Mouse Macrophages to Coxiella burnetii Infection In Vitro: Macrophages from Restrictive Strains Control Parasitophorous Vacuole Maturation. Infect. Immun. 72: 2395-2399 [Abstract] [Full Text]  
  • Boulos, A., Rolain, J.-M., Raoult, D. (2004). Antibiotic Susceptibility of Tropheryma whipplei in MRC5 Cells. Antimicrob. Agents Chemother. 48: 747-752 [Abstract] [Full Text]  
  • Zamboni, D. S., Rabinovitch, M. (2003). Nitric Oxide Partially Controls Coxiella burnetii Phase II Infection in Mouse Primary Macrophages. Infect. Immun. 71: 1225-1233 [Abstract] [Full Text]  
  • Hoover, T. A., Culp, D. W., Vodkin, M. H., Williams, J. C., Thompson, H. A. (2002). Chromosomal DNA Deletions Explain Phenotypic Characteristics of Two Antigenic Variants, Phase II and RSA 514 (Crazy), of the Coxiella burnetii Nine Mile Strain. Infect. Immun. 70: 6726-6733 [Abstract] [Full Text]  
  • Ghigo, E., Capo, C., Tung, C.-H., Raoult, D., Gorvel, J.-P., Mege, J.-L. (2002). Coxiellaburnetii Survival in THP-1 Monocytes Involves the Impairment of Phagosome Maturation: IFN-{gamma} Mediates its Restoration and Bacterial Killing. J. Immunol. 169: 4488-4495 [Abstract] [Full Text]  
  • Beron, W., Gutierrez, M. G., Rabinovitch, M., Colombo, M. I. (2002). Coxiella burnetii Localizes in a Rab7-Labeled Compartment with Autophagic Characteristics. Infect. Immun. 70: 5816-5821 [Abstract] [Full Text]  
  • Webster, P. (2002). Early Intracellular Events During Internalization of Listeria monocytogenes by J774 Cells. J. Histochem. Cytochem. 50: 503-518 [Abstract] [Full Text]  
  • Maurin, M., Raoult, D. (2001). Use of Aminoglycosides in Treatment of Infections Due to Intracellular Bacteria. Antimicrob. Agents Chemother. 45: 2977-2986 [Full Text]  
  • Sturgill-Koszycki, S., Swanson, M. S. (2000). Legionella pneumophila Replication Vacuoles Mature into Acidic, Endocytic Organelles. JEM 192: 1261-1272 [Abstract] [Full Text]  
  • Howe, D., Mallavia, L. P. (2000). Coxiella burnetii Exhibits Morphological Change and Delays Phagolysosomal Fusion after Internalization by J774A.1 Cells. Infect. Immun. 68: 3815-3821 [Abstract] [Full Text]  
  • Hashim, S., Mukherjee, K., Raje, M., Basu, S. K., Mukhopadhyay, A. (2000). Live Salmonella Modulate Expression of Rab Proteins to Persist in a Specialized Compartment and Escape Transport to Lysosomes. J. Biol. Chem. 275: 16281-16288 [Abstract] [Full Text]  
  • Mukherjee, K., Siddiqi, S. A., Hashim, S., Raje, M., Basu, S. K., Mukhopadhyay, A. (2000). Live Salmonella Recruits N-Ethylmaleimide-Sensitive Fusion Protein on Phagosomal Membrane and Promotes Fusion with Early Endosome. JCB 148: 741-754 [Abstract] [Full Text]  
  • Maurin, M., Raoult, D. (1999). Q Fever. Clin. Microbiol. Rev. 12: 518-553 [Abstract] [Full Text]  
  • Porte, F., Liautard, J.-P., Kohler, S. (1999). Early Acidification of Phagosomes Containing Brucella suis Is Essential for Intracellular Survival in Murine Macrophages. Infect. Immun. 67: 4041-4047 [Abstract] [Full Text]  
  • Gomes, M. S., Paul, S., Moreira, A. L., Appelberg, R., Rabinovitch, M., Kaplan, G. (1999). Survival of Mycobacterium avium and Mycobacterium tuberculosis in Acidified Vacuoles of Murine Macrophages. Infect. Immun. 67: 3199-3206 [Abstract] [Full Text]  
  • Howe, D., Mallavia, L. P. (1999). Coxiella burnetii Infection Increases Transferrin Receptors on J774A.1 Cells. Infect. Immun. 67: 3236-3241 [Abstract] [Full Text]  
  • Raoult, D., Houpikian, P., Dupont, H. T., Riss, J. M., Arditi-Djiane, J., Brouqui, P. (1999). Treatment of Q Fever Endocarditis: Comparison of 2 Regimens Containing Doxycycline and Ofloxacin or Hydroxychloroquine. Arch Intern Med 159: 167-173 [Abstract] [Full Text]  
  • Pizarro-Cerda, J., Meresse, S., Parton, R. G., van der Goot, G., Sola-Landa, A., Lopez-Goni, I., Moreno, E., Gorvel, J.-P. (1998). Brucella abortus Transits through the Autophagic Pathway and Replicates in the Endoplasmic Reticulum of Nonprofessional Phagocytes. Infect. Immun. 66: 5711-5724 [Abstract] [Full Text]  
  • Alvarez-Dominguez, C, Roberts, R, Stahl, P. (1997). Internalized Listeria monocytogenes modulates intracellular trafficking and delays maturation of the phagosome. J. Cell Sci. 110: 731-743 [Abstract]  
  • Veras, P., de Chastellier, C, Moreau, M., Villiers, V, Thibon, M, Mattei, D, Rabinovitch, M (1994). Fusion between large phagocytic vesicles: targeting of yeast and other particulates to phagolysosomes that shelter the bacterium Coxiella burnetii or the protozoan Leishmania amazonensis in Chinese hamster ovary cells. J. Cell Sci. 107: 3065-3076 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»