This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Finlay, B B
Right arrow Articles by Kaper, J B
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Finlay, B B
Right arrow Articles by Kaper, J B

 Previous Article  |  Next Article 

Infect Immun. 1992 June; 60(6): 2541-2543

Cytoskeletal composition of attaching and effacing lesions associated with enteropathogenic Escherichia coli adherence to HeLa cells.

B B Finlay, I Rosenshine, M S Donnenberg and J B Kaper

Biotechnology Laboratory, University of British Columbia, Vancouver, Canada.

ABSTRACT

The cytoskeletal lesions associated with enteropathogenic Escherichia coli adhering to cultured HeLa epithelial cells were examined by immunofluorescence microscopy. The microfilament-associated proteins actin, alpha-actinin, talin, and ezrin were localized with adherent enteropathogenic E. coli, whereas tropomyosin, keratin and vimentin (intermediate filaments), tubulin (microtubules), and vinculin were not localized. These cytoskeletal structures differed significantly from those associated with Salmonella typhimurium internalization (invasion).

Infect Immun. 1992 June; 60(6): 2541-2543




This article has been cited by other articles:

  • Lacher, D. W., Steinsland, H., Blank, T. E., Donnenberg, M. S., Whittam, T. S. (2007). Molecular Evolution of Typical Enteropathogenic Escherichia coli: Clonal Analysis by Multilocus Sequence Typing and Virulence Gene Allelic Profiling. J. Bacteriol. 189: 342-350 [Abstract] [Full Text]  
  • Garrido, P., Blanco, M., Moreno-Paz, M., Briones, C., Dahbi, G., Blanco, J., Blanco, J., Parro, V. (2006). STEC-EPEC Oligonucleotide Microarray: A New Tool for Typing Genetic Variants of the LEE Pathogenicity Island of Human and Animal Shiga Toxin-Producing Escherichia coli (STEC) and Enteropathogenic E. coli (EPEC) Strains. Clin. Chem. 52: 192-201 [Abstract] [Full Text]  
  • Garmendia, J., Frankel, G., Crepin, V. F. (2005). Enteropathogenic and Enterohemorrhagic Escherichia coli Infections: Translocation, Translocation, Translocation. Infect. Immun. 73: 2573-2585 [Full Text]  
  • Barocchi, M. A., Ko, A. I., Reis, M. G., McDonald, K. L., Riley, L. W. (2002). Rapid Translocation of Polarized MDCK Cell Monolayers by Leptospira interrogans, an Invasive but Nonintracellular Pathogen. Infect. Immun. 70: 6926-6932 [Abstract] [Full Text]  
  • Zhang, W. L., Kohler, B., Oswald, E., Beutin, L., Karch, H., Morabito, S., Caprioli, A., Suerbaum, S., Schmidt, H. (2002). Genetic Diversity of Intimin Genes of Attaching and Effacing Escherichia coli Strains. J. Clin. Microbiol. 40: 4486-4492 [Abstract] [Full Text]  
  • Kenny, B. (2002). Enteropathogenic Escherichia coli (EPEC) - a crafty subversive little bug. Microbiology 148: 1967-1978 [Full Text]  
  • Simonovic, I., Arpin, M., Koutsouris, A., Falk-Krzesinski, H. J., Hecht, G. (2001). Enteropathogenic Escherichia coli Activates Ezrin, Which Participates in Disruption of Tight Junction Barrier Function. Infect. Immun. 69: 5679-5688 [Abstract] [Full Text]  
  • Elliott, D. A., Coleman, D. J., Lane, M. A., May, R. C., Machesky, L. M., Clark, D. P. (2001). Cryptosporidium parvum Infection Requires Host Cell Actin Polymerization. Infect. Immun. 69: 5940-5942 [Abstract] [Full Text]  
  • KUMAR, S. S., SANKARAN, K., HAIGH, R., WILLIAMS, P. H., BALAKRISHNAN, A. (2001). Cytopathic effects of outer-membrane preparations of enteropathogenic Escherichia coli and co-expression of maltoporin with secretory virulence factor, EspB. J Med Microbiol 50: 602-612 [Abstract] [Full Text]  
  • Goosney, D. L., DeVinney, R., Finlay, B. B. (2001). Recruitment of Cytoskeletal and Signaling Proteins to Enteropathogenic and Enterohemorrhagic Escherichia coli Pedestals. Infect. Immun. 69: 3315-3322 [Abstract] [Full Text]  
  • Fuller, J. D., Bast, D. J., Nizet, V., Low, D. E., de Azavedo, J. C. S. (2001). Streptococcus iniae Virulence Is Associated with a Distinct Genetic Profile. Infect. Immun. 69: 1994-2000 [Abstract] [Full Text]  
  • Ceu Sousa, M., Goncalves, C. A., Bairos, V. A., Poiares-da-Silva, J. (2001). Adherence of Giardia lamblia Trophozoites to Int-407 Human Intestinal Cells. CVI 8: 258-265 [Abstract] [Full Text]  
  • Czerucka, D., Dahan, S., Mograbi, B., Rossi, B., Rampal, P. (2001). Implication of Mitogen-Activated Protein Kinases in T84 Cell Responses to Enteropathogenic Escherichia coli Infection. Infect. Immun. 69: 1298-1305 [Abstract] [Full Text]  
  • Czerucka, D., Dahan, S., Mograbi, B., Rossi, B., Rampal, P. (2000). Saccharomyces boulardii Preserves the Barrier Function and Modulates the Signal Transduction Pathway Induced in Enteropathogenic Escherichia coli-Infected T84 Cells. Infect. Immun. 68: 5998-6004 [Abstract] [Full Text]  
  • Manjarrez-Hernandez, H. A., Gavilanes-Parra, S., Chavez-Berrocal, E., Navarro-Ocana, A., Cravioto, A. (2000). Antigen Detection in Enteropathogenic Escherichia coli Using Secretory Immunoglobulin A Antibodies Isolated from Human Breast Milk. Infect. Immun. 68: 5030-5036 [Abstract] [Full Text]  
  • Winner, F., Rosengarten, R., Citti, C. (2000). In Vitro Cell Invasion of Mycoplasma gallisepticum. Infect. Immun. 68: 4238-4244 [Abstract] [Full Text]  
  • Marches, O., Nougayrede, J.-P., Boullier, S., Mainil, J., Charlier, G., Raymond, I., Pohl, P., Boury, M., De Rycke, J., Milon, A., Oswald, E. (2000). Role of Tir and Intimin in the Virulence of Rabbit Enteropathogenic Escherichia coli Serotype O103:H2. Infect. Immun. 68: 2171-2182 [Abstract] [Full Text]  
  • Elliott, D. A., Clark, D. P. (2000). Cryptosporidium parvum Induces Host Cell Actin Accumulation at the Host-Parasite Interface. Infect. Immun. 68: 2315-2322 [Abstract] [Full Text]  
  • Cantarelli, V. V., Takahashi, A., Akeda, Y., Nagayama, K., Honda, T. (2000). Interaction of Enteropathogenic or Enterohemorrhagic Escherichia coli with HeLa Cells Results in Translocation of Cortactin to the Bacterial Adherence Site. Infect. Immun. 68: 382-386 [Abstract] [Full Text]  
  • Goluszko, P., Selvarangan, R., Popov, V., Pham, T., Wen, J. W., Singhal, J. (1999). Decay-Accelerating Factor and Cytoskeleton Redistribution Pattern in HeLa Cells Infected with Recombinant Escherichia coli Strains Expressing Dr Family of Adhesins. Infect. Immun. 67: 3989-3997 [Abstract] [Full Text]  
  • DeVinney, R., Stein, M., Reinscheid, D., Abe, A., Ruschkowski, S., Finlay, B. B. (1999). Enterohemorrhagic Escherichia coli O157:H7 Produces Tir, Which Is Translocated to the Host Cell Membrane but Is Not Tyrosine Phosphorylated. Infect. Immun. 67: 2389-2398 [Abstract] [Full Text]  
  • Taylor, K. A., Luther, P. W., Donnenberg, M. S. (1999). Expression of the EspB Protein of Enteropathogenic Escherichia coli within HeLa Cells Affects Stress Fibers and Cellular Morphology. Infect. Immun. 67: 120-125 [Abstract] [Full Text]  
  • Adu-Bobie, J., Trabulsi, L. R., Carneiro-Sampaio, M. M. S., Dougan, G., Frankel, G. (1998). Identification of Immunodominant Regions within the C-Terminal Cell Binding Domain of Intimin alpha  and Intimin beta  from Enteropathogenic Escherichia coli. Infect. Immun. 66: 5643-5649 [Abstract] [Full Text]  
  • Abe, A., Heczko, U., Hegele, R. G., Brett Finlay, B. (1998). Two Enteropathogenic Escherichia coli Type III Secreted Proteins, EspA and EspB, Are Virulence Factors. JEM 188: 1907-1916 [Abstract] [Full Text]  
  • Taylor, K. A., O'Connell, C. B., Luther, P. W., Donnenberg, M. S. (1998). The EspB Protein of Enteropathogenic Escherichia coli Is Targeted to the Cytoplasm of Infected HeLa Cells. Infect. Immun. 66: 5501-5507 [Abstract] [Full Text]  
  • Borowsky, M. L., Hynes, R. O. (1998). Layilin, A Novel Talin-binding Transmembrane Protein Homologous with C-type Lectins, is Localized in Membrane Ruffles. JCB 143: 429-442 [Abstract] [Full Text]  
  • Coxon, P. Y., Summersgill, J. T., Ramirez, J. A., Miller, R. D. (1998). Signal Transduction during Legionella pneumophila Entry into Human Monocytes. Infect. Immun. 66: 2905-2913 [Abstract] [Full Text]  
  • Hueck, C. J. (1998). Type III Protein Secretion Systems in Bacterial Pathogens of Animals and Plants. Microbiol. Mol. Biol. Rev. 62: 379-433 [Abstract] [Full Text]  
  • Lamarcq, L. H., McFall-Ngai, M. J. (1998). Induction of a Gradual, Reversible Morphogenesis of Its Host's Epithelial Brush Border by Vibrio fischeri. Infect. Immun. 66: 777-785 [Abstract] [Full Text]  
  • Donnenberg, M. S., Tacket, C. O., Losonsky, G., Frankel, G., Nataro, J. P., Dougan, G., Levine, M. M. (1998). Effect of Prior Experimental Human Enteropathogenic Escherichia coli Infection on Illness following Homologous and Heterologous Rechallenge. Infect. Immun. 66: 52-58 [Abstract] [Full Text]  
  • Nataro, J. P., Kaper, J. B. (1998). Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201 [Abstract] [Full Text]  
  • Frankel, G., Lider, O., Hershkoviz, R., Mould, A. P., Kachalsky, S. G., Candy, D. C.A., Cahalon, L., Humphries, M. J., Dougan, G. (1996). The Cell-binding Domain of Intimin from Enteropathogenic Escherichia coli Binds to beta 1 Integrins. J. Biol. Chem. 271: 20359-20364 [Abstract] [Full Text]  
  • Finlay, B.B. (1995). Interactions Between Salmonella Typhimurium, Enteropathogenic Escherichia Coli (EPEC), and Host Epithelial Cells. ADR 9: 31-36 [Abstract]  
  • Garcia-del Portillo, F., Pucciarelli, M. G., Jefferies, W. A., Finlay, B. B. (1994). Salmonella typhimurium induces selective aggregation and internalization of host cell surface proteins during invasion of epithelial cells. J. Cell Sci. 107: 2005-2020 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»