This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by McOrist, S Articles by Lawson, G H Search for Related Content PubMed PubMed Citation Articles by McOrist, S Articles by Lawson, G H

Reproduction of porcine proliferative enteropathy with pure cultures of ileal symbiont intracellularis.

Department of Veterinary Pathology, University of Edinburgh, Easter Bush, Midlothian, Scotland.

ABSTRACT

Porcine proliferative enteropathy is consistently associated with the presence of intracellular curved bacteria in epithelial cells in affected portions of intestine. Two strains of these intracellular bacteria were cultured in a cell culture system with rat enterocytes (IEC-18) and passaged several times and used as oral inocula for 14 gnotobiotic and 8 conventional pigs. DNA and immunological studies had identified these bacteria as belonging to a new taxon, Ileal symbiont (IS) intracellularis. Conventional pigs dosed with approximately 3.7 x 10(6) of these organisms passaged six times in cell culture developed severe lesions of proliferative enteropathy in the ileum. Other conventional pigs dosed with a lower titer or with organisms passaged 13 times developed moderate and minor lesions, respectively. All gnotobiotic pigs dosed with organisms failed to develop lesions. Control pigs, eight conventional and two gnotobiotic, dosed with diluent, uninfected cell material or left undosed failed to develop lesions also. Reisolation of IS intracellularis and demonstration of the organism in mucosal and fecal samples only occurred in conventional pigs dosed with organisms. Gnotobiotic pigs lacking a normal intestinal flora have not been shown to be colonized by the organism. Seroconversion to IS intracellularis or mucosal infiltration by inflammatory cells was not observed in experimentally affected pigs, confirming the weak immune response characteristic of the natural disease. These results support the identification of IS intracellularis as an etiological agent of proliferative enteropathy in pigs.

This article has been cited by other articles:

• Meeusen, E. N. T., Walker, J., Peters, A., Pastoret, P.-P., Jungersen, G. (2007). Current Status of Veterinary Vaccines. Clin. Microbiol. Rev. 20: 489-510 [Abstract] [Full Text]  
• Whitney, M. H., Shurson, G. C., Guedes, R. C. (2006). Effect of dietary inclusion of distillers dried grains with solubles, soybean hulls, or a polyclonal antibody product on the ability of growing pigs to resist a Lawsonia intracellularis challenge. J ANIM SCI 84: 1880-1889 [Abstract] [Full Text]  
• Gustafsdottir, S. M., Nordengrahn, A., Fredriksson, S., Wallgren, P., Rivera, E., Schallmeiner, E., Merza, M., Landegren, U. (2006). Detection of individual microbial pathogens by proximity ligation.. Clin. Chem. 52: 1152-1160 [Abstract] [Full Text]  
• Kroll, J. J., Eichmeyer, M. A., Schaeffer, M. L., McOrist, S., Harris, D. L., Roof, M. B. (2005). Lipopolysaccharide-Based Enzyme-Linked Immunosorbent Assay for Experimental Use in Detection of Antibodies to Lawsonia intracellularis in Pigs. CVI 12: 693-699 [Abstract] [Full Text]  
• Ott, S J, Musfeldt, M, Wenderoth, D F, Hampe, J, Brant, O, Folsch, U R, Timmis, K N, Schreiber, S (2004). Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease. Gut 53: 685-693 [Abstract] [Full Text]  
• MacIntyre, N., Smith, D. G. E., Shaw, D. J., Thomson, J. R., Rhind, S. M. (2003). Immunopathogenesis of Experimentally Induced Proliferative Enteropathy in Pigs. Vet Pathol 40: 421-432 [Abstract] [Full Text]  
• Smith, D. G. E., Mitchell, S. C., Nash, T., Rhind, S. (2000). Gamma Interferon Influences Intestinal Epithelial Hyperplasia Caused by Lawsonia intracellularis Infection in Mice. Infect. Immun. 68: 6737-6743 [Abstract] [Full Text]  
• Leser, T. D., Lindecrona, R. H., Jensen, T. K., Jensen, B. B., Møller, K. (2000). Changes in Bacterial Community Structure in the Colon of Pigs Fed Different Experimental Diets and after Infection with Brachyspira hyodysenteriae. Appl. Environ. Microbiol. 66: 3290-3296 [Abstract] [Full Text]