This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pancholi, P Articles by Bhardwaj, N Search for Related Content PubMed PubMed Citation Articles by Pancholi, P Articles by Bhardwaj, N

 Previous Article  |  Next Article 

Infect Immun. 1993 December; 61(12): 5326-5332

Presentation of mycobacterial antigens by human dendritic cells: lack of transfer from infected macrophages.

Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York.

ABSTRACT

When exposed to a challenge of 10 Mycobacterium bovis BCG cells per antigen-presenting cell, most human monocytes engulf several organisms. In contrast, blood dendritic cells which are potent antigen-presenting cells for several antigens are not detectably phagocytic for mycobacteria. We investigated the possibility that infected macrophages might regurgitate antigens for presentation by populations of human blood dendritic cells. Macrophages were infected with M. bovis BCG, mixed with uninfected dendritic cells, and added to immune T cells, either bulk T cells or cloned populations from BCG vaccinees or patients recovering from tuberculosis. The macrophages were from donors who were mismatched to the T cells so that transfer of antigen to major histocompatibility complex-matched dendritic cells could be evaluated. As we describe, there was no evidence for the transfer of mycobacterial antigens from macrophages to dendritic cells in a form that was stimulatory for the T cells.

This article has been cited by other articles:

• Ciavarra, R. P., Stephens, A., Nagy, S., Sekellick, M., Steel, C. (2006). Evaluation of Immunological Paradigms in a Virus Model: Are Dendritic Cells Critical for Antiviral Immunity and Viral Clearance?. J. Immunol. 177: 492-500 [Abstract] [Full Text]  
• Newman, S. L., Holly, A. (2001). Candida albicans Is Phagocytosed, Killed, and Processed for Antigen Presentation by Human Dendritic Cells. Infect. Immun. 69: 6813-6822 [Abstract] [Full Text]  
• Flynn, J. L., Chan, J. (2001). Tuberculosis: Latency and Reactivation. Infect. Immun. 69: 4195-4201 [Full Text]  
• Gildea, L. A., Morris, R. E., Newman, S. L. (2001). Histoplasma capsulatum Yeasts Are Phagocytosed Via Very Late Antigen-5, Killed, and Processed for Antigen Presentation by Human Dendritic Cells. J. Immunol. 166: 1049-1056 [Abstract] [Full Text]  
• Fortsch, D., Rollinghoff, M., Stenger, S. (2000). IL-10 Converts Human Dendritic Cells into Macrophage-Like Cells with Increased Antibacterial Activity Against Virulent Mycobacterium tuberculosis. J. Immunol. 165: 978-987 [Abstract] [Full Text]  
• Delvig, A. A., Robinson, J. H. (1998). Two T Cell Epitopes from the M5 Protein of Viable Streptococcus pyogenes Engage Different Pathways of Bacterial Antigen Processing in Mouse Macrophages. J. Immunol. 160: 5267-5272 [Abstract] [Full Text]  
• Hart, D. N.J. (1997). Dendritic Cells: Unique Leukocyte Populations Which Control the Primary Immune Response. Blood 90: 3245-3287 [Full Text]