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Infect Immun. 1993 May; 61(5): 1694-1699

Pore-forming properties of the major 53-kilodalton surface antigen from the outer sheath of Treponema denticola.

Department of Microbiology, University of British Columbia, Vancouver, Canada.

ABSTRACT

A 53-kDa protein from the outer sheath of the oral spirochete Treponema denticola was purified to homogeneity and shown to reconstitute channels in black lipid bilayer model membranes. The channel had a single-channel conductance of 1.8 nS in 0.1 M KCl, making this the largest porin channel observed to date (estimated diameter, 3.4 nm). Electron micrographs of 53-kDa-protein-containing outer sheaths of T. denticola showed a regular hexagonal array of darker staining pits.

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