This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hogh, B Articles by Sherman, I W Search for Related Content PubMed PubMed Citation Articles by Hogh, B Articles by Sherman, I W

research-article

Immune responses to band 3 neoantigens on Plasmodium falciparum-infected erythrocytes in subjects living in an area of intense malaria transmission are associated with low parasite density and high hematocrit value.

Laboratory of Parasitology, Statens Seruminstitut, Copenhagen, Denmark.

ABSTRACT

During the intracellular development of the human malarial parasite, Plasmodium falciparum, cryptic regions of the erythrocyte band 3 protein are exposed. Antibodies against these band 3-related neoantigens block cytoadherence, and peptides based on amino acid sequences of putative exofacial loops of band 3 protein block the in vitro and in vivo adherence of P. falciparum-infected erythrocytes. At present, it is not known whether reactivity to these antigens is related to exposure to the malaria parasite or is correlated with protective immunity. The reactivities of plasma to peptides containing amino acid sequences of putative exofacial loops 3 and 7 of human band 3 protein were determined for children and adults living in an area of perennial malaria transmission (Liberia) and for donors who had never been exposed to malaria (Denmark). Plasma samples from children and adults living in an area of intense malaria transmission showed a much higher reactivity with the band 3 peptides than did those from nonimmune individuals. High reactivity to the loop 3 peptide (amino acids 546 to 555) was correlated with lower mean parasite density in children in the 5- to 9-year-old age group. The presence of antibodies against loop 3 and 7 peptides was not associated with a low packed erythrocyte volume (hematocrit); in fact, higher-than-average reactivities to both peptides were positively correlated with high hematocrit values, indicating that antibodies which specifically recognize the band 3-related neoantigens are not involved in hemolysis (autoimmunity).

This article has been cited by other articles:

• Luginbuhl, A., Nikolic, M., Beck, H. P., Wahlgren, M., Lutz, H. U. (2007). Complement Factor D, Albumin, and Immunoglobulin G Anti-Band 3 Protein Antibodies Mimic Serum in Promoting Rosetting of Malaria-Infected Red Blood Cells. Infect. Immun. 75: 1771-1777 [Abstract] [Full Text]  
• KAY, M. (2005). Immunoregulation of Cellular Life Span. Ann. N. Y. Acad. Sci. 1057: 85-111 [Abstract] [Full Text]  
• Tokumasu, F., Fairhurst, R. M., Ostera, G. R., Brittain, N. J., Hwang, J., Wellems, T. E., Dvorak, J. A. (2005). Band 3 modifications in Plasmodium falciparum-infected AA and CC erythrocytes assayed by autocorrelation analysis using quantum dots. J. Cell Sci. 118: 1091-1098 [Abstract] [Full Text]  
• Jarolim, P., Rubin, H.L., Zakova, D., Storry, J., Reid, M.E. (1998). Characterization of Seven Low Incidence Blood Group Antigens Carried by Erythrocyte Band 3 Protein. Blood 92: 4836-4843 [Abstract] [Full Text]  
• Jakobsen, P. H., McKay, V., N'Jie, R., Olaleye, B. O., D'Alessandro, U., Zhang, G.-H., Eggelte, T. A., Koch, C., Greenwood, B. M. (1998). Decreased Antitoxic Activities among Children with Clinical Episodes of Malaria. Infect. Immun. 66: 1654-1659 [Abstract] [Full Text]