This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by de Kort, G Articles by van de Klundert, J A Search for Related Content PubMed PubMed Citation Articles by de Kort, G Articles by van de Klundert, J A

 Previous Article  |  Next Article 

Infection and Immunity, November 1994, p. 4722-4726, Vol. 62, No. 11
0019-9567/1994/$04.00+0     DOI:

research-article

Invasion of rabbit ileal tissue by Enterobacter cloacae varies with the concentration of OmpX in the outer membrane.

Department of Medical Microbiology, University Hospital, Leiden, The Netherlands.

ABSTRACT

The outer membrane protein OmpX of Enterobacter cloacae shows high amino acid homology with virulence proteins PagC and Rck from Salmonella typhimurium and with Ail from Yersinia enterocolitica. Here we demonstrate a role for OmpX in the invasion of rabbit ileal tissue by E. cloacae. An organ culture system was used for maintenance of rabbit gut tissue during the experiments. The invasiveness of three E. cloacae strains, which differed in OmpX content, were compared with each other and with that of Salmonella typhimurium TML (a highly invasive strain) and S. typhimurium LT7 (a noninvasive strain). There was no significant difference between the invasiveness of the wild type and that of an ompX deletion mutant strain of E. cloacae; they were equally as invasive or less invasive than S. typhimurium LT7. The invasiveness of an OmpX overproducer strain of E. cloacae was 10-fold higher than that of its immediate parent carrying only the multicopy plasmid, higher than that of S. typhimurium LT7, but lower than that of S. typhimurium TML. The invasiveness of E. cloacae thus varied directly with the level of OmpX in the outer membrane in rabbit ileal enterocytes challenged in situ.

This article has been cited by other articles:

• Brown, E. A., Hardwidge, P. R. (2007). Biochemical characterization of the enterotoxigenic Escherichia coli LeoA protein. Microbiology 153: 3776-3784 [Abstract] [Full Text]  
• Otto, K., Hermansson, M. (2004). Inactivation of ompX Causes Increased Interactions of Type 1 Fimbriated Escherichia coli with Abiotic Surfaces. J. Bacteriol. 186: 226-234 [Abstract] [Full Text]  
• ROBEY, M., MORGAN, E., LODGE, J. M., BOLTON, A. J., MARTIN, G. D., BROWN, N. L., STEPHEN, J. (2002). A new chromosomal locus associated with gut-modulated phenotypes in Salmonella enterica serotype Typhimurium. J Med Microbiol 51: 247-263 [Abstract] [Full Text]  
• Fernández, C., Adeishvili, K., Wüthrich, K. (2001). Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles. Proc. Natl. Acad. Sci. USA 10.1073/pnas.051629298v1 [Abstract] [Full Text]  
• Keller, R., Pedroso, M. Z., Ritchmann, R., Silva, R. M. (1998). Occurrence of Virulence-Associated Properties in Enterobacter cloacae. Infect. Immun. 66: 645-649 [Abstract] [Full Text]  
• Fernandez, C., Adeishvili, K., Wuthrich, K. (2001). Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles. Proc. Natl. Acad. Sci. USA 98: 2358-2363 [Abstract] [Full Text]