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Infection and Immunity, February 1994, p. 757-758, Vol. 62, No. 2
0019-9567/1994/$04.00+0     DOI:

research-article

Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis.

Department of Microbiology, University of Leicester, United Kingdom.

ABSTRACT

The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.

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