This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kobayashi, H Articles by Suzuki, S Search for Related Content PubMed PubMed Citation Articles by Kobayashi, H Articles by Suzuki, S

 Previous Article  |  Next Article 

Infection and Immunity, March 1994, p. 968-973, Vol. 62, No. 3
0019-9567/1994/$04.00+0     DOI:

research-article

Structural modification of cell wall mannans of Candida albicans serotype A strains grown in yeast extract-Sabouraud liquid medium under acidic conditions.

Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan.

ABSTRACT

The cell wall mannans of two Candida albicans serotype A strains, NIH A-207 and J-1012 (abbreviated as A and J strains, respectively), cultured in yeast extract-Sabouraud liquid medium at pH 2.0, contained neither a phosphate group nor a beta-1,2-linked mannopyranose unit (H. Kobayashi, P. Giummelly, S. Takahashi, M. Ishida, J. Sato, M. Takaku, Y. Nishidate, N. Shibata, Y. Okawa, and S. Suzuki, Biochem. Biophys. Res. Commun. 175:1003-1009, 1991). In this study, the mannans obtained from A and J strains grown in pH 2.0 medium (abbreviated as mannans A2 and J2, respectively) exhibited quite different reactivities against rabbit anti-C. albicans and anti-Saccharomyces cerevisiae sera compared with those of mannans from the corresponding strains cultured in conventional medium at pH 5.9 (abbreviated as mannans A and J, respectively). Namely, mannans A2 and J2 lost reactivity against the former serum but reacted with the latter serum to a higher extent than mannans A and J. In order to account for these difference in more detail, mannans A2 and J2 were subjected to acetolysis. Elution profiles of the acetolysates were completely different from those of acetolysates obtained from mannans A and J reported in our previous papers. The 1H nuclear magnetic resonance spectra of the oligosaccharides from mannans A2 and J2 obtained by this procedure indicate that the side chains are composed of alpha-linked mannopyranose units densely linked to the alpha-1,6-linked backbone. The long side chains containing one alpha-1,3-linked mannopyranose unit are markedly increased.

This article has been cited by other articles:

• Hazen, K. C, Singleton, D. R, Masuoka, J. (2007). Influence of outer region mannosylphosphorylation on N-glycan formation by Candida albicans: Normal acid-stable N-glycan formation requires acid-labile mannosylphosphate addition. Glycobiology 17: 1052-1060 [Abstract] [Full Text]  
• Mille, C., Janbon, G., Delplace, F., Ibata-Ombetta, S., Gaillardin, C., Strecker, G., Jouault, T., Trinel, P.-A., Poulain, D. (2004). Inactivation of CaMIT1 Inhibits Candida albicans Phospholipomannan {beta}-Mannosylation, Reduces Virulence, and Alters Cell Wall Protein {beta}-Mannosylation. J. Biol. Chem. 279: 47952-47960 [Abstract] [Full Text]  
• Masuoka, J., Hazen, K. C. (2004). Cell Wall Mannan and Cell Surface Hydrophobicity in Candida albicans Serotype A and B Strains. Infect. Immun. 72: 6230-6236 [Abstract] [Full Text]  
• Masuoka, J. (2004). Surface Glycans of Candida albicans and Other Pathogenic Fungi: Physiological Roles, Clinical Uses, and Experimental Challenges. Clin. Microbiol. Rev. 17: 281-310 [Abstract] [Full Text]  
• Han, Y., Morrison, R. P., Cutler, J. E. (1998). A Vaccine and Monoclonal Antibodies That Enhance Mouse Resistance to Candida albicans Vaginal Infection. Infect. Immun. 66: 5771-5776 [Abstract] [Full Text]  
• Martinez, J. P., Gil, M. L., Lopez-Ribot, J. L., Chaffin, W. L. (1998). Serologic Response to Cell Wall Mannoproteins and Proteins of Candida albicans. Clin. Microbiol. Rev. 11: 121-141 [Abstract] [Full Text]