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Infection and Immunity, April 1994, p. 1213-1218, Vol. 62, No. 4
0019-9567/1994/$04.00+0     DOI:

research-article

Size heterogeneity among antigenically related Giardia lamblia variant-specific surface proteins is due to differences in tandem repeat copy number.

M R Mowatt, B Y Nguyen, J T Conrad, R D Adam, and T E Nash

Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

ABSTRACT

Giardia lamblia undergoes antigenic variation by modulating the expression of the different genes that comprise the trophozoite's variant-specific surface protein (VSP) repertoire. We studied an epitope that is conserved among VSPs expressed by cloned trophozoite lines derived from the independent G. lamblia isolates WB, G3M, Be-2, and CAT. The epitope recognized by monoclonal antibody 6E7 lies entirely within the region of tandemly repeated 65-amino-acid units that is characteristic of these size-variant VSPs. Northern (RNA) hybridization, cDNA cloning, and DNA sequence analysis indicate that size heterogeneity among these VSPs is due to differences in the number of repetitive units.

Infection and Immunity, April 1994, p. 1213-1218, Vol. 62, No. 4
0019-9567/1994/$04.00+0     DOI:




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