This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pascopella, L Articles by Jacobs, W R Search for Related Content PubMed PubMed Citation Articles by Pascopella, L Articles by Jacobs, W R, Jr

research-article

Use of in vivo complementation in Mycobacterium tuberculosis to identify a genomic fragment associated with virulence.

Department of Microbiology and Immunology, Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York 10461.

ABSTRACT

Novel molecular tools and genetic methods were developed to isolate genomic fragments of Mycobacterium tuberculosis that may be associated with virulence. We sought to restore virulence, a characteristic of M. tuberculosis that is correlated with growth rate in mouse spleen and lung tissue, to the avirulent strain H37Ra by complementation. A representative library of the virulent M. tuberculosis strain H37Rv was constructed and transformed into H37Ra. Enrichment for individual faster-growing recombinants was achieved by passage of pools of H37Ra transformants harboring the H37Rv library through mice. A molecular strategy was devised to isolate and clone the H37Rv genomic DNA fragment ivg, which conferred a more rapid in vivo growth rate to H37Ra.

This article has been cited by other articles:

• Chesne-Seck, M.-L., Barilone, N., Boudou, F., Asensio, J. G., Kolattukudy, P. E., Martin, C., Cole, S. T., Gicquel, B., Gopaul, D. N., Jackson, M. (2008). A Point Mutation in the Two-Component Regulator PhoP-PhoR Accounts for the Absence of Polyketide-Derived Acyltrehaloses but Not That of Phthiocerol Dimycocerosates in Mycobacterium tuberculosis H37Ra. J. Bacteriol. 190: 1329-1334 [Abstract] [Full Text]  
• Subbian, S., Mehta, P. K., Cirillo, S. L. G., Bermudez, L. E., Cirillo, J. D. (2007). A Mycobacterium marinum mel2 Mutant Is Defective for Growth in Macrophages That Produce Reactive Oxygen and Reactive Nitrogen Species. Infect. Immun. 75: 127-134 [Abstract] [Full Text]  
• Casali, N., White, A. M., Riley, L. W. (2006). Regulation of the Mycobacterium tuberculosis mce1 Operon. J. Bacteriol. 188: 441-449 [Abstract] [Full Text]  
• Collins, D. M., Skou, B., White, S., Bassett, S., Collins, L., For, R., Hurr, K., Hotter, G., de Lisle, G. W. (2005). Generation of Attenuated Mycobacterium bovis Strains by Signature-Tagged Mutagenesis for Discovery of Novel Vaccine Candidates. Infect. Immun. 73: 2379-2386 [Abstract] [Full Text]  
• Mills, J. A., Motichka, K., Jucker, M., Wu, H. P., Uhlik, B. C., Stern, R. J., Scherman, M. S., Vissa, V. D., Pan, F., Kundu, M., Ma, Y. F., McNeil, M. (2004). Inactivation of the Mycobacterial Rhamnosyltransferase, Which Is Needed for the Formation of the Arabinogalactan-Peptidoglycan Linker, Leads to Irreversible Loss of Viability. J. Biol. Chem. 279: 43540-43546 [Abstract] [Full Text]  
• Collins, D. M., Kawakami, R. P., Buddle, B. M., Wards, B. J., de Lisle, G. W. (2003). Different susceptibility of two animal species infected with isogenic mutants of Mycobacterium bovis identifies phoT as having roles in tuberculosis virulence and phosphate transport. Microbiology 149: 3203-3212 [Abstract] [Full Text]  
• Smith, I. (2003). Mycobacterium tuberculosis Pathogenesis and Molecular Determinants of Virulence. Clin. Microbiol. Rev. 16: 463-496 [Abstract] [Full Text]  
• Rindi, L., Fattorini, L., Bonanni, D., Iona, E., Freer, G., Tan, D., Deho, G., Orefici, G., Garzelli, C. (2002). Involvement of the fadD33 gene in the growth of Mycobacterium tuberculosis in the liver of BALB/c mice. Microbiology 148: 3873-3880 [Abstract] [Full Text]  
• Howard, S. T., Byrd, T. F., Lyons, C. R. (2002). A polymorphic region in Mycobacterium abscessus contains a novel insertion sequence element. Microbiology 148: 2987-2996 [Abstract] [Full Text]  
• Dullaghan, E. M., Malloff, C. A., Li, A. H., Lam, W. L., Stokes, R. W. (2002). Two-dimensional bacterial genome display: a method for the genomic analysis of mycobacteria. Microbiology 148: 3111-3117 [Abstract] [Full Text]  
• Feng, Z., Caceres, N. E., Sarath, G., Barletta, R. G. (2002). Mycobacterium smegmatisL-Alanine Dehydrogenase (Ald) Is Required for Proficient Utilization of Alanine as a Sole Nitrogen Source and Sustained Anaerobic Growth. J. Bacteriol. 184: 5001-5010 [Abstract] [Full Text]  
• Soto, C. Y., Andreu, N., Gibert, I., Luquin, M. (2002). Simple and Rapid Differentiation of Mycobacterium tuberculosis H37Ra from M. tuberculosis Clinical Isolates through Two Cytochemical Tests Using Neutral Red and Nile Blue Stains. J. Clin. Microbiol. 40: 3021-3024 [Abstract] [Full Text]  
• Chacon, O., Feng, Z., Harris, N. B., Caceres, N. E., Adams, L. G., Barletta, R. G. (2002). Mycobacterium smegmatisD-Alanine Racemase Mutants Are Not Dependent on D-Alanine for Growth. Antimicrob. Agents Chemother. 46: 47-54 [Abstract] [Full Text]  
• Smith, H. E., Buijs, H., Wisselink, H. J., Stockhofe-Zurwieden, N., Smits, M. A. (2001). Selection of Virulence-Associated Determinants of Streptococcus suis Serotype 2 by In Vivo Complementation. Infect. Immun. 69: 1961-1966 [Abstract] [Full Text]  
• Monahan, I. M., Betts, J., Banerjee, D. K., Butcher, P. D. (2001). Differential expression of mycobacterial proteins following phagocytosis by macrophages. Microbiology 147: 459-471 [Abstract] [Full Text]  
• Alland, D., Steyn, A. J., Weisbrod, T., Aldrich, K., Jacobs, W. R. Jr. (2000). Characterization of the Mycobacterium tuberculosis iniBAC Promoter, a Promoter That Responds to Cell Wall Biosynthesis Inhibition. J. Bacteriol. 182: 1802-1811 [Abstract] [Full Text]  
• Wei, J., Dahl, J. L., Moulder, J. W., Roberts, E. A., O'Gaora, P., Young, D. B., Friedman, R. L. (2000). Identification of a Mycobacterium tuberculosis Gene That Enhances Mycobacterial Survival in Macrophages. J. Bacteriol. 182: 377-384 [Abstract] [Full Text]  
• Miller, B. H., Shinnick, T. M. (2000). Evaluation of Mycobacterium tuberculosis Genes Involved in Resistance to Killing by Human Macrophages. Infect. Immun. 68: 387-390 [Abstract] [Full Text]  
• Brosch, R., Philipp, W. J., Stavropoulos, E., Colston, M. J., Cole, S. T., Gordon, S. V. (1999). Genomic Analysis Reveals Variation between Mycobacterium tuberculosis H37Rv and the Attenuated M. tuberculosis H37Ra Strain. Infect. Immun. 67: 5768-5774 [Abstract] [Full Text]  
• Joshi, A. D., Swanson, M. S. (1999). Comparative Analysis of Legionella pneumophila and Legionella micdadei Virulence Traits. Infect. Immun. 67: 4134-4142 [Abstract] [Full Text]  
• Pelicic, V., Jackson, M., Reyrat, J.-M., Jacobs, W. R. Jr., Gicquel, B., Guilhot, C. (1997). Efficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 94: 10955-10960 [Abstract] [Full Text]