This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vidotto, M C Articles by Knowles, D P Search for Related Content PubMed PubMed Citation Articles by Vidotto, M C Articles by Knowles, D P, Jr

 Previous Article  |  Next Article 

Infection and Immunity, July 1994, p. 2940-2946, Vol. 62, No. 7
0019-9567/1994/$04.00+0     DOI:

research-article

Intermolecular relationships of major surface proteins of Anaplasma marginale.

Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.

ABSTRACT

Immunization with Anaplasma marginale membranes containing major surface proteins (MSPs) induces protective immunity against clinical disease (N. Tebele, T. C. McGuire, and G. H. Palmer, Infect. Immun. 59:3199-3204, 1991). For use in design of a recombinant antigen subunit vaccine for A. marginale, intermolecular relationships of known A. marginale MSPs were analyzed. Under nonreducing conditions, MSP-2 and MSP-5 occur as multimers. A large (> 300-kDa-molecular-mass), nonreduced protein complex contained MSP-1a linked by disulfide bonds to MSP-1b and by noncovalent bonds to MSP-5. MSP-2 was also noncovalently bound to this complex. The nearest neighbor membrane proteins were identified by cross-linking reactions followed by immunoblotting with anti-MSP antibodies. A cross-linked aggregate retained in the stacking gel contained MSP-1a, MSP-1b, MSP-2, MSP-3, MSP-4, and MSP-5. Collectively, the data indicate that MSP-2 and MSP-5 occur as monomers and disulfide-bonded multimers. The MSP-1 complex occurs as both disulfide-bonded and noncovalently associated MSP-1 and MSP-1b, and MSP-2 and MSP-5 are noncovalently associated with MSP-1. Also, MSP-1, MSP-2, MSP-3, and MSP-4 are nearest neighbors, and MSP-5 is noncovalently associated with this cross-linked complex.

This article has been cited by other articles:

• Macmillan, H., Norimine, J., Brayton, K. A., Palmer, G. H., Brown, W. C. (2008). Physical Linkage of Naturally Complexed Bacterial Outer Membrane Proteins Enhances Immunogenicity. Infect. Immun. 76: 1223-1229 [Abstract] [Full Text]  
• Lopez, J. E., Palmer, G. H., Brayton, K. A., Dark, M. J., Leach, S. E., Brown, W. C. (2007). Immunogenicity of Anaplasma marginale Type IV Secretion System Proteins in a Protective Outer Membrane Vaccine. Infect. Immun. 75: 2333-2342 [Abstract] [Full Text]  
• Macmillan, H., Brayton, K. A., Palmer, G. H., McGuire, T. C., Munske, G., Siems, W. F., Brown, W. C. (2006). Analysis of the Anaplasma marginale Major Surface Protein 1 Complex Protein Composition by Tandem Mass Spectrometry. J. Bacteriol. 188: 4983-4991 [Abstract] [Full Text]  
• Noh, S. M., Brayton, K. A., Knowles, D. P., Agnes, J. T., Dark, M. J., Brown, W. C., Baszler, T. V., Palmer, G. H. (2006). Differential Expression and Sequence Conservation of the Anaplasma marginale msp2 Gene Superfamily Outer Membrane Proteins.. Infect. Immun. 74: 3471-3479 [Abstract] [Full Text]  
• Mavromatis, K., Doyle, C. K., Lykidis, A., Ivanova, N., Francino, M. P., Chain, P., Shin, M., Malfatti, S., Larimer, F., Copeland, A., Detter, J. C., Land, M., Richardson, P. M., Yu, X. J., Walker, D. H., McBride, J. W., Kyrpides, N. C. (2006). The Genome of the Obligately Intracellular Bacterium Ehrlichia canis Reveals Themes of Complex Membrane Structure and Immune Evasion Strategies. J. Bacteriol. 188: 4015-4023 [Abstract] [Full Text]  
• Brayton, K. A., Kappmeyer, L. S., Herndon, D. R., Dark, M. J., Tibbals, D. L., Palmer, G. H., McGuire, T. C., Knowles, D. P. Jr. (2005). Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins. Proc. Natl. Acad. Sci. USA 102: 844-849 [Abstract] [Full Text]  
• Kocan, K. M., de la Fuente, J., Guglielmone, A. A., Melendez, R. D. (2003). Antigens and Alternatives for Control of Anaplasma marginale Infection in Cattle. Clin. Microbiol. Rev. 16: 698-712 [Abstract] [Full Text]  
• Park, J., Choi, K. S., Dumler, J. S. (2003). Major Surface Protein 2 of Anaplasma phagocytophilum Facilitates Adherence to Granulocytes. Infect. Immun. 71: 4018-4025 [Abstract] [Full Text]  
• Brown, W. C., McGuire, T. C., Mwangi, W., Kegerreis, K. A., Macmillan, H., Lewin, H. A., Palmer, G. H. (2002). Major Histocompatibility Complex Class II DR-Restricted Memory CD4+ T Lymphocytes Recognize Conserved Immunodominant Epitopes of Anaplasma marginale Major Surface Protein 1a. Infect. Immun. 70: 5521-5532 [Abstract] [Full Text]  
• McBride, J. W., Ndip, L. M., Popov, V. L., Walker, D. H. (2002). Identification and Functional Analysis of an Immunoreactive DsbA-Like Thio-Disulfide Oxidoreductase of Ehrlichia spp.. Infect. Immun. 70: 2700-2703 [Abstract] [Full Text]  
• Brown, W. C., Palmer, G. H., Lewin, H. A., McGuire, T. C. (2001). CD4+ T Lymphocytes from Calves Immunized with Anaplasma marginale Major Surface Protein 1 (MSP1), a Heteromeric Complex of MSP1a and MSP1b, Preferentially Recognize the MSP1a Carboxyl Terminus That Is Conserved among Strains. Infect. Immun. 69: 6853-6862 [Abstract] [Full Text]  
• Camacho-Nuez, M., de Lourdes Munoz, M., Suarez, C. E., McGuire, T. C., Brown, W. C., Palmer, G. H. (2000). Expression of Polymorphic msp1beta Genes during Acute Anaplasma marginale Rickettsemia. Infect. Immun. 68: 1946-1952 [Abstract] [Full Text]  
• French, D. M., Brown, W. C., Palmer, G. H. (1999). Emergence of Anaplasma marginale Antigenic Variants during Persistent Rickettsemia. Infect. Immun. 67: 5834-5840 [Abstract] [Full Text]  
• Arulkanthan, A., Brown, W. C., McGuire, T. C., Knowles, D. P. (1999). Biased Immunoglobulin G1 Isotype Responses Induced in Cattle with DNA Expressing msp1a of Anaplasma marginale. Infect. Immun. 67: 3481-3487 [Abstract] [Full Text]  
• Barbet, A. F., Blentlinger, R., Yi, J., Lundgren, A. M., Blouin, E. F., Kocan, K. M. (1999). Comparison of Surface Proteins of Anaplasma marginale Grown in Tick Cell Culture, Tick Salivary Glands, and Cattle. Infect. Immun. 67: 102-107 [Abstract] [Full Text]  
• Munodzana, D., McElwain, T. F., Knowles, D. P., Palmer, G. H. (1998). Conformational Dependence of Anaplasma marginale Major Surface Protein 5 Surface-Exposed B-Cell Epitopes. Infect. Immun. 66: 2619-2624 [Abstract] [Full Text]  
• French, D. M., McElwain, T. F., McGuire, T. C., Palmer, G. H. (1998). Expression of Anaplasma marginale Major Surface Protein 2 Variants during Persistent Cyclic Rickettsemia. Infect. Immun. 66: 1200-1207 [Abstract] [Full Text]