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Infection and Immunity, August 1994, p. 3184-3188, Vol. 62, No. 8
0019-9567/1994/$04.00+0     DOI:

research-article

Interaction of the two components of leukocidin from Staphylococcus aureus with human polymorphonuclear leukocyte membranes: sequential binding and subsequent activation.

D A Colin, I Mazurier, S Sire, and V Finck-Barbançon

Laboratoire de Toxinologie Bactérienne, Faculté de Médecine, Université Louis Pasteur, Strasbourg, France.

ABSTRACT

The sequential interaction between the two components S and F of leukocidin from Staphylococcus aureus and the membrane of human polymorphonuclear neutrophils has been investigated in the presence of 1 mM Ca2+. With 125I-labeled components, it has been shown that binding of the F component occurred only after binding of the S component. The kinetic constants of binding of both components were not statistically different (Kd, approximately 5 nM; Bm, approximately 35,000 molecules per cell), and both Hill coefficients were 1. The application of increasing concentrations of leukocidin provoked a dose-dependent secretion of the granule content, as determined by hexosaminidase and lysozyme activity measurements. Furthermore, the separate perfusion of S and F components on human polymorphonuclear neutrophils deposited on a filter induced secretion of the granules content only when the perfusion of the S component preceded that of the F component. We conclude, therefore, that (i) S-component binding is a prerequisite for F-component binding and for subsequent activation of polymorphonuclear neutrophils and (ii) there is a specific binding site for the S component in the plasma membrane.

Infection and Immunity, August 1994, p. 3184-3188, Vol. 62, No. 8
0019-9567/1994/$04.00+0     DOI:




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