This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Walker, K E Articles by Weiss, A A Search for Related Content PubMed PubMed Citation Articles by Walker, K E Articles by Weiss, A A

research-article

Characterization of the dermonecrotic toxin in members of the genus Bordetella.

Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, Ohio 45267.

ABSTRACT

All members of the genus Bordetella and Pasteurella multocida (a gram-negative bacillus genetically unrelated to Bordetella spp., yet often sharing the same ecological niche) produce a dermonecrotic toxin (DNT). The amount of toxin produced and the time required for appearance of the lesions are identical for Bordetella pertussis, B. parapertussis, and B. bronchiseptica but different for P. multocida and B. avium. DNT has been reported to act by promoting vasoconstriction; however, vasoactive compounds (verapamil, prazosin, hydralazine, tolazoline, or isoxsuprine) are able to reverse the action of the toxin only slightly. Vasoconstrictors (atropine, serotonin, epinephrine, or endothelin) did not produce DNT-like lesions. We have characterized a region of DNA essential for DNT expression. We have determined by Southern analysis that the restriction map of the DNT gene is nearly identical in B. pertussis, B. parapertussis, and B. bronchiseptica, but the sequences are not present in toxigenic B. avium and P. multocida strains. A gentamicin resistance-origin of transfer cassette cloned into a 1.8-kb NotI-BamHI fragment results in constructs which can be mobilized and recombined into the Bordetella chromosome, rendering the resultant B. pertussis, B. parapertussis, and B. bronchiseptica strains negative for DNT. A 5-kb BamHI-ApaI fragment from the B. pertussis chromosome was sequenced and revealed homology to the Escherichia coli CNF1 (cytotoxic necrotizing factor 1) toxin.

This article has been cited by other articles:

• Reissinger, A., Skinner, J. A., Yuk, M. H. (2005). Downregulation of Mitogen-Activated Protein Kinases by the Bordetella bronchiseptica Type III Secretion System Leads to Attenuated Nonclassical Macrophage Activation. Infect. Immun. 73: 308-316 [Abstract] [Full Text]  
• Bagley, K. C., Abdelwahab, S. F., Tuskan, R. G., Lewis, G. K. (2005). Pasteurella multocida Toxin Activates Human Monocyte-Derived and Murine Bone Marrow-Derived Dendritic Cells In Vitro but Suppresses Antibody Production In Vivo. Infect. Immun. 73: 413-421 [Abstract] [Full Text]  
• Nogawa, H., Kuwae, A., Matsuzawa, T., Abe, A. (2004). The Type III Secreted Protein BopD in Bordetella bronchiseptica Is Complexed with BopB for Pore Formation on the Host Plasma Membrane. J. Bacteriol. 186: 3806-3813 [Abstract] [Full Text]  
• Rambow-Larsen, A. A., Weiss, A. A. (2004). Temporal Expression of Pertussis Toxin and Ptl Secretion Proteins by Bordetella pertussis. J. Bacteriol. 186: 43-50 [Abstract] [Full Text]  
• Spears, P. A., Temple, L. M., Miyamoto, D. M., Maskell, D. J., Orndorff, P. E. (2003). Unexpected Similarities between Bordetella avium and Other Pathogenic Bordetellae. Infect. Immun. 71: 2591-2597 [Abstract] [Full Text]  
• Shime, H., Ohnishi, T., Nagao, K., Oka, K., Takao, T., Horiguchi, Y. (2002). Association of Pasteurella multocida Toxin with Vimentin. Infect. Immun. 70: 6460-6463 [Abstract] [Full Text]  
• Brockmeier, S. L., Register, K. B., Magyar, T., Lax, A. J., Pullinger, G. D., Kunkle, R. A. (2002). Role of the Dermonecrotic Toxin of Bordetella bronchiseptica in the Pathogenesis of Respiratory Disease in Swine. Infect. Immun. 70: 481-490 [Abstract] [Full Text]  
• Pullinger, G. D., Sowdhamini, R., Lax, A. J. (2001). Localization of Functional Domains of the Mitogenic Toxin of Pasteurella multocida. Infect. Immun. 69: 7839-7850 [Abstract] [Full Text]  
• Meysick, K. C., Mills, M., O'Brien, A. D. (2001). Epitope Mapping of Monoclonal Antibodies Capable of Neutralizing Cytotoxic Necrotizing Factor Type 1 of Uropathogenic Escherichia coli. Infect. Immun. 69: 2066-2074 [Abstract] [Full Text]  
• Wilson, B. A., Aminova, L. R., Ponferrada, V. G., Ho, M. (2000). Differential Modulation and Subsequent Blockade of Mitogenic Signaling and Cell Cycle Progression by Pasteurella multocida Toxin. Infect. Immun. 68: 4531-4538 [Abstract] [Full Text]  
• West, N. P., Jungnitz, H., Fitter, J. T., McArthur, J. D., Guzman, C. A., Walker, M. J. (2000). Role of Phosphoglucomutase of Bordetella bronchiseptica in Lipopolysaccharide Biosynthesis and Virulence. Infect. Immun. 68: 4673-4680 [Abstract] [Full Text]  
• Schmidt, G., Goehring, U.-M., Schirmer, J., Lerm, M., Aktories, K. (1999). Identification of the C-terminal Part of Bordetella Dermonecrotic Toxin as a Transglutaminase for Rho GTPases. J. Biol. Chem. 274: 31875-31881 [Abstract] [Full Text]  
• Lerm, M., Schmidt, G., Goehring, U.-M., Schirmer, J., Aktories, K. (1999). Identification of the Region of Rho Involved in Substrate Recognition by Escherichia coli Cytotoxic Necrotizing Factor 1 (CNF1). J. Biol. Chem. 274: 28999-29004 [Abstract] [Full Text]  
• Kashimoto, T., Katahira, J., Cornejo, W. R., Masuda, M., Fukuoh, A., Matsuzawa, T., Ohnishi, T., Horiguchi, Y. (1999). Identification of Functional Domains of Bordetella Dermonecrotizing Toxin. Infect. Immun. 67: 3727-3732 [Abstract] [Full Text]  
• Weingart, C. L., Broitman-Maduro, G., Dean, G., Newman, S., Peppler, M., Weiss, A. A. (1999). Fluorescent Labels Influence Phagocytosis of Bordetella pertussis by Human Neutrophils. Infect. Immun. 67: 4264-4267 [Abstract] [Full Text]  
• Wilson, B. A., Ponferrada, V. G., Vallance, J. E., Ho, M. (1999). Localization of the Intracellular Activity Domain of Pasteurella multocida Toxin to the N Terminus. Infect. Immun. 67: 80-87 [Abstract] [Full Text]  
• Temple, L. M., Weiss, A. A., Walker, K. E., Barnes, H. J., Christensen, V. L., Miyamoto, D. M., Shelton, C. B., Orndorff, P. E. (1998). Bordetella avium Virulence Measured In Vivo and In Vitro. Infect. Immun. 66: 5244-5251 [Abstract] [Full Text]  
• Fernandez-Prada, C., Tall, B. D., Elliott, S. E., Hoover, D. L., Nataro, J. P., Venkatesan, M. M. (1998). Hemolysin-Positive Enteroaggregative and Cell-Detaching Escherichia coli Strains Cause Oncosis of Human Monocyte-Derived Macrophages and Apoptosis of Murine J774 Cells. Infect. Immun. 66: 3918-3924 [Abstract] [Full Text]  
• Schmidt, G., Selzer, J., Lerm, M., Aktories, K. (1998). The Rho-deamidating Cytotoxic Necrotizing Factor 1 from Escherichia coli Possesses Transglutaminase Activity. CYSTEINE 866 AND HISTIDINE 881 ARE ESSENTIAL FOR ENZYME ACTIVITY. J. Biol. Chem. 273: 13669-13674 [Abstract] [Full Text]  
• Horiguchi, Y., Inoue, N., Masuda, M., Kashimoto, T., Katahira, J., Sugimoto, N., Matsuda, M. (1997). Bordetella bronchiseptica dermonecrotizing toxin induces reorganization of actin stress fibers through deamidation of Gln-63 of the GTP-binding protein Rho. Proc. Natl. Acad. Sci. USA 94: 11623-11626 [Abstract] [Full Text]  
• Fiorentini, C., Fabbri, A., Flatau, G., Donelli, G., Matarrese, P., Lemichez, E., Falzano, L., Boquet, P. (1997). Escherichia coli Cytotoxic Necrotizing Factor 1 (CNF1), a Toxin That Activates the Rho GTPase. J. Biol. Chem. 272: 19532-19537 [Abstract] [Full Text]  
• Lacerda, H. M., Pullinger, G. D., Lax, A. J., Rozengurt, E. (1997). Cytotoxic Necrotizing Factor 1from Escherichia coli and Dermonecrotic Toxin from Bordetella bronchiseptica Induce p21rho-dependent Tyrosine Phosphorylation of Focal Adhesion Kinase and Paxillin in Swiss 3T3 Cells. J. Biol. Chem. 272: 9587-9596 [Abstract] [Full Text]