This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vanmaele, R. P. Articles by Armstrong, G. D. Search for Related Content PubMed PubMed Citation Articles by Vanmaele, R. P. Articles by Armstrong, G. D.

 Previous Article  |  Next Article 

Infect. Immun., 01 1995, 191-198, Vol 63, No. 1
Copyright © 1995, American Society for Microbiology

Effect of enteropathogenic Escherichia coli on adherent properties of Chinese hamster ovary cells

RP Vanmaele, MC Finlayson and GD Armstrong
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.

Enteropathogenic Escherichia coli (EPEC) O111:H2, O119:H6, or O142:H6 caused rapid detachment of Chinese hamster ovary (CHO) cell monolayers within 2 to 4 h of cocultivation. CHO cell detachment was not promoted by nonenteropathogenic E. coli (O125:H4, O126:H27, O157:H7, and O26:H11) and could not be attributed to EPEC production of enterohemolysin or Shiga-like toxins. In contrast, EPEC strains did not promote rapid detachment of Lec1, Lec2, or Lec8 CHO cell monolayers. These CHO cell Lec mutants all express abbreviated glycan sequences on membrane glycoproteins and glycolipids. Although EPEC strains failed to alter the adherent properties of Lec2 cells lacking only terminal sialic acid groups, EPEC adherence to the Lec2 mutant was indistinguishable from that observed with wild-type CHO cells. There was also no significant difference in EPEC-induced actin accumulation or invasion of Lec2 cells. In contrast, EPEC localized adherence to Lec1 and Lec8 mutants, lacking sialyllactosamine (Lec1) or sialic acid and galactose (Lec8) sequences, was reduced by 84 and 93%, respectively. Our results suggest that lactosamine sequences [beta Gal(1-4 or 1-3)beta GlcNAc] not containing sialic acid are sufficient for EPEC adherence, actin accumulation, and invasion of CHO cells. Sialic acid groups, however, may be necessary for EPEC-mediated CHO cell detachment.

This article has been cited by other articles:

• Hyland, R. M., Beck, P., Mulvey, G. L., Kitov, P. I., Armstrong, G. D. (2006). N-Acetyllactosamine Conjugated to Gold Nanoparticles Inhibits Enteropathogenic Escherichia coli Colonization of the Epithelium in Human Intestinal Biopsy Specimens. Infect. Immun. 74: 5419-5421 [Abstract] [Full Text]  
• Hyland, R. M., Griener, T. P., Mulvey, G. L., Kitov, P. I., Srivastava, O. P., Marcato, P., Armstrong, G. D. (2006). Basis for N-acetyllactosamine-mediated inhibition of enteropathogenic Escherichia coli localized adherence. J Med Microbiol 55: 669-675 [Abstract] [Full Text]  
• Angeloni, S., Ridet, J.L., Kusy, N., Gao, H., Crevoisier, F., Guinchard, S., Kochhar, S., Sigrist, H., Sprenger, N. (2005). Glycoprofiling with micro-arrays of glycoconjugates and lectins. Glycobiology 15: 31-41 [Abstract] [Full Text]  
• Lai, X.-H., Golovliov, I., Sjostedt, A. (2001). Francisella tularensis Induces Cytopathogenicity and Apoptosis in Murine Macrophages via a Mechanism That Requires Intracellular Bacterial Multiplication. Infect. Immun. 69: 4691-4694 [Abstract] [Full Text]  
• Lai, X.-H., Wang, S.-Y., Uhlin, B. E. (1999). Expression of cytotoxicity by potential pathogens in the standard Escherichia coli collection of reference (ECOR) strains. Microbiology 145: 3295-3303 [Abstract] [Full Text]  
• Vanmaele, R. P., Heerze, L. D., Armstrong, G. D. (1999). Role of Lactosyl Glycan Sequences in Inhibiting Enteropathogenic Escherichia coli Attachment. Infect. Immun. 67: 3302-3307 [Abstract] [Full Text]