This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kasten, R. W. Articles by Hirsh, D. C. Search for Related Content PubMed PubMed Citation Articles by Kasten, R. W. Articles by Hirsh, D. C.

 Previous Article  |  Next Article 

Infect. Immun., Mar 1995, 989-993, Vol 63, No. 3
Copyright © 1995, American Society for Microbiology

Pasteurella multocida produces a protein with homology to the P6 outer membrane protein of Haemophilus influenzae

RW Kasten, LM Hansen, J Hinojoza, D Bieber, WW Ruehl and DC Hirsh
Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis 95616.

An antibody specific for a 16-kDa outer membrane protein of a rabbit strain of Pasteurella multocida was used to probe representatives of all 16 somatic serotypes of P. multocida, as well as the vaccine strains CU and M9, and all were shown to express the protein. The gene encoding this protein was cloned and sequenced and found to have extensive sequence homology with the gene encoding the P6 protein of Haemophilus influenzae. The protein in P. multocida has been designated P6-like. The gene encoding the P6-like protein was used to probe members of the family Pasteurellaceae and other gram-negative bacteria. Representatives of all 16 somatic serotypes (as well as the vaccine strains CU and M9) of P. multocida hybridized with the P6-like gene under conditions of high stringency. The DNA from H. influenzae hybridized weakly with the P6-like gene under these conditions, but Pasteurella haemolytica (representatives of A and T biotypes), Bordetella bronchiseptica, B. avium, Actinobacillus suis, A. suis-like, A. lignieresii, A. ureae, A. rossii, A. pleuropneumoniae, A. equuli, and various members of the family Enterobacteriaceae (Escherichia coli, Klebsiella pneumoniae, and Salmonella typhimurium) did not hybridize detectably. Under conditions of lower stringency, the P6-like gene also hybridized strongly with DNA from P. multocida, H. influenzae, and A. rossii but weakly with DNA from P. haemolytica and members of the genus Actinobacillus. These results suggest that the P6-like protein of P. multocida might be useful as an immunizing product to protect poultry from avian cholera. This suggestion stems from (i) our finding that the P6-like protein in P. multocida is widely distributed among all the somatic serotypes and (ii) the previous work of others demonstrating that the P6 protein of H. influenzae elicits a protective immune response in animal models of human disease.

This article has been cited by other articles:

• Xia, X., Wei, T., Xie, Z., Danchin, A. (2002). Genomic Changes in Nucleotide and Dinucleotide Frequencies in Pasteurella multocida Cultured Under High Temperature. Genetics 161: 1385-1394 [Abstract] [Full Text]