This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Guo, Z. Articles by Kaneshiro, E. S. Search for Related Content PubMed PubMed Citation Articles by Guo, Z. Articles by Kaneshiro, E. S.

 Previous Article  |  Next Article 

Infect. Immun., 04 1995, 1286-1290, Vol 63, No. 4
Copyright © 1995, American Society for Microbiology

Phospholipid composition of Pneumocystis carinii carinii and effects of methylprednisolone immunosuppression on rat lung lipids

Z Guo and ES Kaneshiro
Department of Biological Sciences, University of Cincinnati, Ohio 45221.

The phospholipid class composition of Pneumocystis carinii carinii freshly isolated from infected lungs generally resembled that of the host lung, suggesting that the parasite scavenges lung alveolar lipids. However, subtle quantitative differences were demonstrated, indicating that the pathogen has the metabolic capacity to de novo synthesize, or at least tailor, its lipids. The concentration of phosphatidylcholine, the major lung surfactant lipid, in the organism was lower than that in lungs of normal and immunosuppressed uninfected rats, and the concentration of phosphatidylinositol was higher. Phosphonolipids were not detected in the organism by chemical analysis and nuclear magnetic resonance spectrometry. The immunosuppressive regimen alone caused increases in both surfactant protein A and the lipid content of the whole lung. The lungs of rats that were subjected to corticosteroid immunosuppression and had heavy parasite loads had dramatically elevated surfactant protein A levels, whereas the lipid contents of these lungs were not different from lipid contents in whole lungs of immunosuppressed uninfected rats. P. carinii was found to concentrate lipids, indicating that a large amount of the lipids in the whole infected rat lung was within the parasites residing in the organ. These observations have important implications relevant to the use of corticosteroid therapy for P. carinii pneumonitis.

This article has been cited by other articles:

• Wang, Z., Foye, A., Chang, Y., Chess, P. R., Wright, T. W., Bhagwat, S., Gigliotti, F., Notter, R. H. (2005). Inhibition of surfactant activity by Pneumocystis carinii organisms and components in vitro. Am. J. Physiol. Lung Cell. Mol. Physiol. 288: L1124-L1131 [Abstract] [Full Text]  
• Maskell, N A, Waine, D J, Lindley, A, Pepperell, J C T, Wakefield, A E, Miller, R F, Davies, R J O (2003). Asymptomatic carriage of Pneumocystis jiroveci in subjects undergoing bronchoscopy: a prospective study. Thorax 58: 594-597 [Abstract] [Full Text]  
• Guadiz, G., Haidaris, C. G., Maine, G. N., Simpson-Haidaris, P. J. (1998). The Carboxyl Terminus of Pneumocystis carinii Glycoprotein A Encodes a Functional Glycosylphosphatidylinositol Signal Sequence. J. Biol. Chem. 273: 26202-26209 [Abstract] [Full Text]  
• Kaneshiro, E. S., Guo, Z., Sul, D., Kallam, K. A., Jayasimhulu, K., Beach, D. H. (1998). Characterizations of Pneumocystis carinii and rat lung lipids: glyceryl ethers and fatty alcohols. J. Lipid Res. 39: 1907-1917 [Abstract] [Full Text]  
• Kaneshiro, E. S. (1998). The Lipids of Pneumocystis carinii. Clin. Microbiol. Rev. 11: 27-41 [Abstract] [Full Text]