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Infect. Immun., Apr 1995, 1318-1328, Vol 63, No. 4
Copyright © 1995, American Society for Microbiology

Aggregative adherence of Klebsiella pneumoniae to human intestine-407 cells

S Favre-Bonte, A Darfeuille-Michaud and C Forestier
Laboratoire de Bacteriologie, Faculte de Pharmacie, Clermont-Ferrand, France.

Aggregative adhesion of Klebsiella pneumoniae LM3 to Intestine-407 (Int- 407) cells was studied. Adhesive capacities were affected by the bacterial growth phase (with a maximum of adherence obtained during the exponential phase), temperature, multiplicity of infection, and length of incubation with Int-407 cells. Adhesion occurred through a cytochalasin D-sensitive process and was greatly reduced after treatment of Int-407 with cycloheximide, indicating that aggregative adhesion requires active participation of Int-407 cells. Transmission electron microscopy revealed that adherent bacteria were surrounded by a capsule-like material, apparently involved in both bacterium-Int-407 cell and bacterium-bacterium adherence. Examination with a scanning electron microscope showed interactions of intestinal cell microvilli with bacteria and formation in 3 h of a fibrous network within and around the bacterial clusters. We speculate that aggregative adhesion of K. pneumoniae mediated by a capsule-like extracellular material might explain the persistence of these strains inside the host gastrointestinal tract.

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