This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fravalo, P. Articles by Bonnaure-Mallet, M. Search for Related Content PubMed PubMed Citation Articles by Fravalo, P. Articles by Bonnaure-Mallet, M.

 Previous Article  |  Next Article 

Infect. Immun., 12 1996, 4940-4945, Vol 64, No. 12
Copyright © 1996, American Society for Microbiology

Effect of Porphyromonas gingivalis on epithelial cell MMP-9 type IV collagenase production

P Fravalo, C Menard and M Bonnaure-Mallet
Equipe de Biologie Buccale, UFR Odontologie, Universite de Rennes I, France.

Porphyromonas gingivalis is reportedly capable of stimulating the expression of host cell matrix metalloproteinases (MMP), contributing to tissue destruction. However, the impact of this bacterium on specific molecules remains to be determined. In this study, we evaluate the effect of P. gingivalis on regulation of MMP-9 expression in human gingival epithelial cells (HGEC). Various inocula of P. gingivalis were added to cultures of HGEC. The effects of live bacteria, heat-killed bacteria, and outer membrane extract were analyzed. MMP-9 secretion by HGEC was evaluated by enzyme-linked immunosorbent assay. For inocula smaller than one bacterium per cell, the quantity of MMP-9 secreted by HGEC was increased in comparison to control conditions. For inocula from 2.5 to 250 bacteria per cell, an inhibition of MMP-9 secretion in a dose-response fashion was observed, with a maximum reduction (ranging from 80 to 95% in five experiments) at 50 bacteria per cell. Gelatin zymograms confirmed the decrease in MMP-9 secretion. A band of 83 kDa, corresponding to activated enzyme, was present for inocula of 0.5 to 50 bacteria. Inhibition took place without any alteration of epithelial cell viability. Heat-killed bacteria and outer membrane extract also provoked proenzyme activation but did not inhibit MMP-9 secretion. These results demonstrate a direct effect of P. gingivalis on HGEC, suggesting a specific action on the collagen renewal process at the interface between the epithelium and connective tissue.

This article has been cited by other articles:

• Andrian, E., Grenier, D., Rouabhia, M. (2006). Porphyromonas gingivalis-Epithelial Cell Interactions in Periodontitis.. J. Dent. Res. 85: 392-403 [Abstract] [Full Text]  
• Li, C. K. F., Pender, S. L. F., Pickard, K. M., Chance, V., Holloway, J. A., Huett, A., Goncalves, N. S., Mudgett, J. S., Dougan, G., Frankel, G., MacDonald, T. T. (2004). Impaired Immunity to Intestinal Bacterial Infection in Stromelysin-1 (Matrix Metalloproteinase-3)-Deficient Mice. J. Immunol. 173: 5171-5179 [Abstract] [Full Text]  
• Lamont, R. J., Jenkinson, H. F. (1998). Life Below the Gum Line: Pathogenic Mechanisms of Porphyromonas gingivalis. Microbiol. Mol. Biol. Rev. 62: 1244-1263 [Abstract] [Full Text]