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Infect. Immun., 01 1997, 309-312, Vol 65, No. 1
Copyright © 1997, American Society for Microbiology

Interaction of Salmonella typhi strains with cultured human monocyte- derived macrophages

DR Sizemore, EA Elsinghorst, LC Eck, AA Branstrom, DL Hoover, RL Warren and FA Rubin
Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100, USA.

Human monocyte-derived macrophages (MDM) provided this laboratory with a tool to develop a primary-cell assay for evaluating the relative virulence of newly constructed Salmonella typhi carrier strains. In this study, the interaction with and survival within MDM were compared for delta aroA143-attenuated strains, wild-type virulent strains, and the current oral-vaccine strain, Ty21a.

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