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Infect. Immun., 03 1997, 1101-1104, Vol 65, No. 3
Copyright © 1997, American Society for Microbiology

Inducible expression of a Porphyromonas gingivalis W83 membrane- associated protease

Y Park, B Lu, C Mazur and BC McBride
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.

The Tpr protease of Porphyromonas gingivalis W83 is a membrane- associated enzyme capable of hydrolyzing a chromogenic bacterial collagenase substrate. An isogenic mutant lacking a functional tpr gene had a greatly reduced ability to hydrolyze the collagenase substrate. Activity was restored to the tpr mutant by introducing a shuttle plasmid containing the tpr gene. Expression of the gene is induced by nutrient limitation, as shown by enzymatic and Northern analyses.


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