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Infect. Immun., 04 1997, 1557-1560, Vol 65, No. 4
Copyright © 1997, American Society for Microbiology

Purification and characterization of a Na+, K+ ATPase inhibitor found in an endotoxin of Leptospira interrogans

P Burth, M Younes-Ibrahim, FH Goncalez, ER Costa and MV Faria
Departamento de Biologia Celular e Genetica, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Brazil.

We showed previously that the glycolipoprotein fraction prepared from Leptospira interrogans inhibited the Na+,K+ ATPase enzyme purified from brain or kidney and in isolated nephron segments (M. Younes-Ibrahim, P. Burth, M. V. Castro Faria, B. Buffin-Meyer, S. Marsy, C. Barlet-Bas, L. Cheval, and A. Doucet, C. R. Acad. Sci. Paris Ser. III 318:619-625, 1995). In the present communication, we have demonstrated that unsaturated fatty acids such as oleic and palmitoleic acids, which are adsorbed to this fraction, are effective inhibitors of the enzyme.