Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential

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Infect. Immun., 07 1997, 2707-2716, Vol 65, No. 7
Copyright © 1997, American Society for Microbiology

Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential

M Wiedmann, JL Bruce, C Keating, AE Johnson, PL McDonough and CA Batt
Department of Food Science, Cornell University, Ithaca, New York 14853, USA. mw16@cornell.edu

A total of 133 Listeria monocytogenes isolates were characterized by ribotyping and allelic analysis of the virulence genes hly, actA, and inlA to uncover linkages between independent phylogenetic and specific virulence markers. PCR-restriction fragment length polymorphisms revealed 8 hly, 11 inl4, and 2 actA alleles. The combination of these virulence gene alleles and ribotype patterns separated L. monocytogenes into three distinct lineages. While distinct hly and inlA alleles were generally found to cluster into these three lineages, actA alleles segregated independently. These three phylogenetic lineages were confirmed when 22 partial actA DNA sequences were analyzed. The clinical history of the L. monocytogenes strains showed evidence for differences in pathogenic potential among the three lineages. Lineage I contains all strains isolated during epidemic outbreaks of listeriosis, while no human isolates were found in lineage III. Animal isolates were found in all three lineages. We found evidence that isolates from lineages I and III have a higher plaquing efficiency than lineage II strains in a cell culture assay. Strains from lineage III also seem to form larger plaques than strains from lineage II. A distinctive ribotype fragment and unique 16S rRNA gene sequences furthermore suggest that lineage III might represent a L. monocytogenes subspecies. None of the 20 human isolates available but 11% of our animal isolates were grouped in this lineage, indicating that strains in this lineage might have reduced virulence for humans.

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Larsen, C. N., Norrung, B., Sommer, H. M., Jakobsen, M. (2002). In Vitro and In Vivo Invasiveness of Different Pulsed-Field Gel Electrophoresis Types of Listeria monocytogenes. Appl. Environ. Microbiol. 68: 5698-5703 [Abstract] [Full Text]
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Mereghetti, L., Lanotte, P., Savoye-Marczuk, V., Marquet-Van Der Mee, N., Audurier, A., Quentin, R. (2002). Combined Ribotyping and Random Multiprimer DNA Analysis To Probe the Population Structure of Listeria monocytogenes. Appl. Environ. Microbiol. 68: 2849-2857 [Abstract] [Full Text]
Czuprynski, C. J., Faith, N. G., Steinberg, H. (2002). Ability of the Listeria monocytogenes Strain Scott A To Cause Systemic Infection in Mice Infected by the Intragastric Route. Appl. Environ. Microbiol. 68: 2893-2900 [Abstract] [Full Text]
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Tran, H. L., Kathariou, S. (2002). Restriction Fragment Length Polymorphisms Detected with Novel DNA Probes Differentiate among Diverse Lineages of Serogroup 4 Listeria monocytogenes and Identify Four Distinct Lineages in Serotype 4b. Appl. Environ. Microbiol. 68: 59-64 [Abstract] [Full Text]
Vela, A. I., Fernandez-Garayzabal, J. F., Vazquez, J. A., Latre, M. V., Blanco, M. M., Moreno, M. A., de la Fuente, L., Marco, J., Franco, C., Cepeda, A., Rodriguez Moure, A. A., Suarez, G., Dominguez, L. (2001). Molecular Typing by Pulsed-Field Gel Electrophoresis of Spanish Animal and Human Listeria monocytogenes Isolates. Appl. Environ. Microbiol. 67: 5840-5843 [Abstract] [Full Text]
Machner, M. P., Urbanke, C., Barzik, M., Otten, S., Sechi, A. S., Wehland, J., Heinz, D. W. (2001). ActA from Listeria monocytogenes Can Interact with Up to Four Ena/VASP Homology 1 Domains Simultaneously. J. Biol. Chem. 276: 40096-40103 [Abstract] [Full Text]
Skoble, J., Auerbuch, V., Goley, E. D., Welch, M. D., Portnoy, D. A. (2001). Pivotal role of VASP in Arp2/3 complex-mediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility. J. Cell Biol. 155: 89-100 [Abstract] [Full Text]
Auerbuch, V., Lenz, L. L., Portnoy, D. A. (2001). Development of a Competitive Index Assay To Evaluate the Virulence of Listeria monocytogenes actA Mutants during Primary and Secondary Infection of Mice. Infect. Immun. 69: 5953-5957 [Abstract] [Full Text]
De Cesare, A., Bruce, J. L., Dambaugh, T. R., Guerzoni, M. E., Wiedmann, M. (2001). Automated Ribotyping Using Different Enzymes To Improve Discrimination of Listeria monocytogenes Isolates, with a Particular Focus on Serotype 4b Strains. J. Clin. Microbiol. 39: 3002-3005 [Abstract] [Full Text]
Vazquez-Boland, J. A., Kuhn, M., Berche, P., Chakraborty, T., Dominguez-Bernal, G., Goebel, W., Gonzalez-Zorn, B., Wehland, J., Kreft, J. (2001). Listeria Pathogenesis and Molecular Virulence Determinants. Clin. Microbiol. Rev. 14: 584-640 [Abstract] [Full Text]
Nadon, C. A., Woodward, D. L., Young, C., Rodgers, F. G., Wiedmann, M. (2001). Correlations between Molecular Subtyping and Serotyping of Listeria monocytogenes. J. Clin. Microbiol. 39: 2704-2707 [Abstract] [Full Text]
Jeffers, G. T., Bruce, J. L., McDonough, P. L., Scarlett, J., Boor, K. J., Wiedmann, M. (2001). Comparative genetic characterization of Listeria monocytogenes isolates from human and animal listeriosis cases. Microbiology 147: 1095-1104 [Abstract] [Full Text]
Norton, D. M., Scarlett, J. M., Horton, K., Sue, D., Thimothe, J., Boor, K. J., Wiedmann, M. (2001). Characterization and Pathogenic Potential of Listeria monocytogenes Isolates from the Smoked Fish Industry. Appl. Environ. Microbiol. 67: 646-653 [Abstract] [Full Text]
Weed, S. A., Karginov, A. V., Schafer, D. A., Weaver, A. M., Kinley, A. W., Cooper, J. A., Parsons, J. T. (2000). Cortactin Localization to Sites of Actin Assembly in Lamellipodia Requires Interactions with F-Actin and the Arp2/3 Complex. J. Cell Biol. 151: 29-40 [Abstract] [Full Text]
Musser, J. M., Amin, A., Ramaswamy, S. (2000). Negligible Genetic Diversity of Mycobacterium tuberculosis Host Immune System Protein Targets: Evidence of Limited Selective Pressure. Genetics 155: 7-16 [Abstract] [Full Text]
Jalal, S., Ciofu, O., Høiby, N., Gotoh, N., Wretlind, B. (2000). Molecular Mechanisms of Fluoroquinolone Resistance in Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients. Antimicrob. Agents Chemother. 44: 710-712 [Abstract] [Full Text]
McDonough, P. L., Fogelman, D., Shin, S. J., Brunner, M. A., Lein, D. H. (1999). Salmonella enterica Serotype Dublin Infection: an Emerging Infectious Disease for the Northeastern United States. J. Clin. Microbiol. 37: 2418-2427 [Abstract] [Full Text]
Wiedmann, M., Arvik, T. J., Hurley, R. J., Boor, K. J. (1998). General Stress Transcription Factor sigma B and Its Role in Acid Tolerance and Virulence of Listeria monocytogenes. J. Bacteriol. 180: 3650-3656 [Abstract] [Full Text]

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