Binding of pulmonary surfactant proteins A and D to Aspergillus fumigatus conidia enhances phagocytosis and killing by human neutrophils and alveolar macrophages

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Madan, T.
	Articles by Reid, K. B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Madan, T.
	Articles by Reid, K. B.

Infect. Immun., 08 1997, 3171-3179, Vol 65, No. 8
Copyright © 1997, American Society for Microbiology

Binding of pulmonary surfactant proteins A and D to Aspergillus fumigatus conidia enhances phagocytosis and killing by human neutrophils and alveolar macrophages

T Madan, P Eggleton, U Kishore, P Strong, SS Aggrawal, PU Sarma and KB Reid
CSIR Centre for Biochemical Technology, Delhi, India.

To determine whether the lung surfactant proteins A (SP-A) and D (SP-D) are involved in the initial protective immunity against opportunistic pulmonary fungal infections caused by Aspergillus fumigatus, we performed a series of in vitro functional studies to see if SP-A and SP- D enhanced binding, phagocytosis, activation, and killing of A. fumigatus conidia by human alveolar macrophages and circulating neutrophils. Both SP-A and SP-D bound to carbohydrate structures on A. fumigatus conidia in a calcium-dependent manner. SP-A and SP-D were also chemoattractant and significantly enhanced agglutination and binding of conidia to alveolar macrophages and neutrophils. Furthermore, in the presence of SP-A and SP-D, the phagocytosis, oxidative burst, and killing of A. fumigatus conidia by neutrophils were significantly increased. These findings indicate that SP-A and SP- D may have an important immunological role in the early antifungal defense responses in the lung, through inhibiting infectivity of conidia by agglutination and by enhancing uptake and killing of A. fumigatus by phagocytic cells.

This article has been cited by other articles:

Mahajan, L., Madan, T., Kamal, N., Singh, V. K., Sim, R. B., Telang, S. D., Ramchand, C. N., Waters, P., Kishore, U., Sarma, P. U. (2008). Recombinant surfactant protein-D selectively increases apoptosis in eosinophils of allergic asthmatics and enhances uptake of apoptotic eosinophils by macrophages. Int Immunol 0: dxn058v1-dxn058 [Abstract] [Full Text]
Sin, D. D., Pahlavan, P. S., Man, S.F. P. (2008). Review: Surfactant protein D: A lung specific biomarker in COPD?. Therapeutic Advances in Respiratory Disease 2: 65-74 [Abstract]
Samarakoon, P., Soubani, A. (2008). Invasive pulmonary aspergillosis in patients with COPD: a report of five cases and systematic review of the literature. Chronic Respiratory Disease 5: 19-27 [Abstract]
Feldmesser, M. (2006). Role of Neutrophils in Invasive Aspergillosis{triangledown}. Infect. Immun. 74: 6514-6516 [Full Text]
Lekkala, M., LeVine, A. M., Linke, M. J., Crouch, E. C., Linders, B., Brummer, E., Stevens, D. A. (2006). Effect of Lung Surfactant Collectins on Bronchoalveolar Macrophage Interaction with Blastomyces dermatitidis: Inhibition of Tumor Necrosis Factor Alpha Production by Surfactant Protein D.. Infect. Immun. 74: 4549-4556 [Abstract] [Full Text]
Haczku, A., Cao, Y., Vass, G., Kierstein, S., Nath, P., Atochina-Vasserman, E. N., Scanlon, S. T., Li, L., Griswold, D. E., Chung, K. F., Poulain, F. R., Hawgood, S., Beers, M. F., Crouch, E. C. (2006). IL-4 and IL-13 Form a Negative Feedback Circuit with Surfactant Protein-D in the Allergic Airway Response. J. Immunol. 176: 3557-3565 [Abstract] [Full Text]
Khamri, W., Moran, A. P., Worku, M. L., Karim, Q. N., Walker, M. M., Annuk, H., Ferris, J. A., Appelmelk, B. J., Eggleton, P., Reid, K. B. M., Thursz, M. R. (2005). Variations in Helicobacter pylori Lipopolysaccharide To Evade the Innate Immune Component Surfactant Protein D. Infect. Immun. 73: 7677-7686 [Abstract] [Full Text]
Malherbe, D. C., Erpenbeck, V. J., Abraham, S. N., Crouch, E. C., Hohlfeld, J. M., Wright, J. R. (2005). Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation. Am. J. Physiol. Lung Cell. Mol. Physiol. 289: L856-L866 [Abstract] [Full Text]
Yang, C.-H., Szeliga, J., Jordan, J., Faske, S., Sever-Chroneos, Z., Dorsett, B., Christian, R. E., Settlage, R. E., Shabanowitz, J., Hunt, D. F., Whitsett, J. A., Chroneos, Z. C. (2005). Identification of the Surfactant Protein A Receptor 210 as the Unconventional Myosin 18A. J. Biol. Chem. 280: 34447-34457 [Abstract] [Full Text]
Casey, J., Kaplan, J., Atochina-Vasserman, E. N., Gow, A. J., Kadire, H., Tomer, Y., Fisher, J. H., Hawgood, S., Savani, R. C., Beers, M. F. (2005). Alveolar Surfactant Protein D Content Modulates Bleomycin-induced Lung Injury. Am. J. Respir. Crit. Care Med. 172: 869-877 [Abstract] [Full Text]
Dave, V., Childs, T., Whitsett, J. A. (2004). Nuclear Factor of Activated T Cells Regulates Transcription of the Surfactant Protein D Gene (Sftpd) via Direct Interaction with Thyroid Transcription Factor-1 in Lung Epithelial Cells. J. Biol. Chem. 279: 34578-34588 [Abstract] [Full Text]
Hirche, T. O., Crouch, E. C., Espinola, M., Brokelman, T. J., Mecham, R. P., DeSilva, N., Cooley, J., Remold-O'Donnell, E., Belaaouaj, A. (2004). Neutrophil Serine Proteinases Inactivate Surfactant Protein D by Cleaving within a Conserved Subregion of the Carbohydrate Recognition Domain. J. Biol. Chem. 279: 27688-27698 [Abstract] [Full Text]
Jounblat, R., Kadioglu, A., Iannelli, F., Pozzi, G., Eggleton, P., Andrew, P. W. (2004). Binding and Agglutination of Streptococcus pneumoniae by Human Surfactant Protein D (SP-D) Vary between Strains, but SP-D Fails To Enhance Killing by Neutrophils. Infect. Immun. 72: 709-716 [Abstract] [Full Text]
van de Wetering, J. K., Coenjaerts, F. E. J., Vaandrager, A. B., van Golde, L. M. G., Batenburg, J. J. (2004). Aggregation of Cryptococcus neoformans by Surfactant Protein D Is Inhibited by Its Capsular Component Glucuronoxylomannan. Infect. Immun. 72: 145-153 [Abstract] [Full Text]
Clark, H, Reid, K (2003). The potential of recombinant surfactant protein D therapy to reduce inflammation in neonatal chronic lung disease, cystic fibrosis, and emphysema. Arch. Dis. Child. 88: 981-984 [Abstract] [Full Text]
Krane, M., Griese, M. (2003). Surfactant protein D in serum from patients with allergic bronchopulmonary aspergillosis. Eur Respir J 22: 592-595 [Abstract] [Full Text]
Lopez, J. P., Clark, E., Shepherd, V. L. (2003). Surfactant protein A enhances Mycobacterium avium ingestion but not killing by rat macrophages. J. Leukoc. Biol. 74: 523-530 [Abstract] [Full Text]
Takeda, K., Miyahara, N., Rha, Y.-H., Taube, C., Yang, E.-S., Joetham, A., Kodama, T., Balhorn, A. M., Dakhama, A., Duez, C., Evans, A. J., Voelker, D. R., Gelfand, E. W. (2003). Surfactant Protein D Regulates Airway Function and Allergic Inflammation through Modulation of Macrophage Function. Am. J. Respir. Crit. Care Med. 168: 783-789 [Abstract] [Full Text]
Philippe, B., Ibrahim-Granet, O., Prevost, M. C., Gougerot-Pocidalo, M. A., Sanchez Perez, M., Van der Meeren, A., Latge, J. P. (2003). Killing of Aspergillus fumigatus by Alveolar Macrophages Is Mediated by Reactive Oxidant Intermediates. Infect. Immun. 71: 3034-3042 [Abstract] [Full Text]
Zhang, L., Hartshorn, K. L., Crouch, E. C., Ikegami, M., Whitsett, J. A. (2002). Complementation of Pulmonary Abnormalities in SP-D(-/-) Mice with an SP-D/Conglutinin Fusion Protein. J. Biol. Chem. 277: 22453-22459 [Abstract] [Full Text]
Chiba, H., Pattanajitvilai, S., Evans, A. J., Harbeck, R. J., Voelker, D. R. (2002). Human Surfactant Protein D (SP-D) Binds Mycoplasma pneumoniae by High Affinity Interactions with Lipids. J. Biol. Chem. 277: 20379-20385 [Abstract] [Full Text]
Murray, E., Khamri, W., Walker, M. M., Eggleton, P., Moran, A. P., Ferris, J. A., Knapp, S., Karim, Q. N., Worku, M., Strong, P., Reid, K. B. M., Thursz, M. R. (2002). Expression of Surfactant Protein D in the Human Gastric Mucosa and during Helicobacter pylori Infection. Infect. Immun. 70: 1481-1487 [Abstract] [Full Text]
Haley, K. J., Ciota, A., Contreras, J. P., Boothby, M. R., Perkins, D. L., Finn, P. W. (2002). Alterations in lung collectins in an adaptive allergic immune response. Am. J. Physiol. Lung Cell. Mol. Physiol. 282: L573-L584 [Abstract] [Full Text]
Ferguson, J. S., Voelker, D. R., Ufnar, J. A., Dawson, A. J., Schlesinger, L. S. (2002). Surfactant Protein D Inhibition of Human Macrophage Uptake of Mycobacterium tuberculosis Is Independent of Bacterial Agglutination. J. Immunol. 168: 1309-1314 [Abstract] [Full Text]
Korfhagen, T. R. (2001). Surfactant Protein A (SP-A)-Mediated Bacterial Clearance . SP-A and Cystic Fibrosis. Am. J. Respir. Cell Mol. Bio. 25: 668-672 [Full Text]
Haczku, A., Atochina, E. N., Tomer, Y., Chen, H., Scanlon, S. T., Russo, S., Xu, J., Panettieri, R. A. Jr., Beers, M. F. (2001). Aspergillus fumigatus-Induced Allergic Airway Inflammation Alters Surfactant Homeostasis and Lung Function in BALB/c Mice. Am. J. Respir. Cell Mol. Bio. 25: 45-50 [Abstract] [Full Text]
von Bredow, C., Birrer, P., Griese, M. (2001). Surfactant protein A and other bronchoalveolar lavage fluid proteins are altered in cystic fibrosis. Eur Respir J 17: 716-722 [Abstract] [Full Text]
Allen, M. J., Voelker, D. R., Mason, R. J. (2001). Interactions of Surfactant Proteins A and D with Saccharomyces cerevisiae and Aspergillus fumigatus. Infect. Immun. 69: 2037-2044 [Abstract] [Full Text]
Madan, T., Kishore, U., Singh, M., Strong, P., Hussain, E. M., Reid, K. B. M., Sarma, P. U. (2001). Protective Role of Lung Surfactant Protein D in a Murine Model of Invasive Pulmonary Aspergillosis. Infect. Immun. 69: 2728-2731 [Abstract] [Full Text]
AWASTHI, S., COALSON, J. J., YODER, B. A., CROUCH, E., KING, R. J. (2001). Deficiencies in Lung Surfactant Proteins A and D Are Associated with Lung Infection in Very Premature Neonatal Baboons. Am. J. Respir. Crit. Care Med. 163: 389-397 [Abstract] [Full Text]
Ofek, I., Mesika, A., Kalina, M., Keisari, Y., Podschun, R., Sahly, H., Chang, D., McGregor, D., Crouch, E. (2001). Surfactant Protein D Enhances Phagocytosis and Killing of Unencapsulated Phase Variants of Klebsiella pneumoniae. Infect. Immun. 69: 24-33 [Abstract] [Full Text]
KRAMER, B. W., JOBE, A. H., BACHURSKI, C. J., IKEGAMI, M. (2001). Surfactant Protein A Recruits Neutrophils into the Lungs of Ventilated Preterm Lambs. Am. J. Respir. Crit. Care Med. 163: 158-165 [Abstract] [Full Text]
Weyer, C., Sabat, R., Wissel, H., Krüger, D. H., Stevens, P. A., Prösch, S. (2000). Surfactant Protein A Binding to Cytomegalovirus Proteins Enhances Virus Entry into Rat Lung Cells. Am. J. Respir. Cell Mol. Bio. 23: 71-78 [Abstract] [Full Text]
Yang, Z., Jaeckisch, S. M, Mitchell, C. G (2000). Enhanced binding of Aspergillus fumigatus spores to A549 epithelial cells and extracellular matrix proteins by a component from the spore surface and inhibition by rat lung lavage fluid. Thorax 55: 579-584 [Abstract] [Full Text]
Madsen, J., Kliem, A., Tornoe, I., Skjodt, K., Koch, C., Holmskov, U. (2000). Localization of Lung Surfactant Protein D on Mucosal Surfaces in Human Tissues. J. Immunol. 164: 5866-5870 [Abstract] [Full Text]
van Eijk, M., Haagsman, H. P., Skinner, T., Archibold, A., Reid, K. B. M., Lawson, P. R. (2000). Porcine Lung Surfactant Protein D: Complementary DNA Cloning, Chromosomal Localization, and Tissue Distribution. J. Immunol. 164: 1442-1450 [Abstract] [Full Text]
Crouch, E. C. (1999). Modulation of Host-Bacterial Interactions by Collectins. Am. J. Respir. Cell Mol. Bio. 21: 558-561 [Full Text]
Restrepo, C. I., Dong, Q., Savov, J., Mariencheck, W. I., Wright, J. R. (1999). Surfactant Protein D Stimulates Phagocytosis of Pseudomonas aeruginosa by Alveolar Macrophages. Am. J. Respir. Cell Mol. Bio. 21: 576-585 [Abstract] [Full Text]
Mariencheck, W. I., Savov, J., Dong, Q., Tino, M. J., Wright, J. R. (1999). Surfactant protein A enhances alveolar macrophage phagocytosis of a live, mucoid strain of P. aeruginosa. Am. J. Physiol. Lung Cell. Mol. Physiol. 277: L777-L786 [Abstract] [Full Text]
Holmskov, U., Mollenhauer, J., Madsen, J., Vitved, L., Gronlund, J., Tornoe, I., Kliem, A., Reid, K. B. M., Poustka, A., Skjodt, K. (1999). Cloning of gp-340, a putative opsonin receptor for lung surfactant protein D. Proc. Natl. Acad. Sci. USA 96: 10794-10799 [Abstract] [Full Text]
Allen, M. J., Harbeck, R., Smith, B., Voelker, D. R., Mason, R. J. (1999). Binding of Rat and Human Surfactant Proteins A and D to Aspergillus fumigatus Conidia. Infect. Immun. 67: 4563-4569 [Abstract] [Full Text]
Ferguson, J. S., Voelker, D. R., McCormack, F. X., Schlesinger, L. S. (1999). Surfactant Protein D Binds to Mycobacterium tuberculosis Bacilli and Lipoarabinomannan via Carbohydrate-Lectin Interactions Resulting in Reduced Phagocytosis of the Bacteria by Macrophages1. J. Immunol. 163: 312-321 [Abstract] [Full Text]
Latge, J.-P. (1999). Aspergillus fumigatus and Aspergillosis. Clin. Microbiol. Rev. 12: 310-350 [Abstract] [Full Text]
Wright, J. R., Zlogar, D. F., Taylor, J. C., Zlogar, T. M., Restrepo, C. I. (1999). Effects of endotoxin on surfactant protein A and D stimulation of NO production by alveolar macrophages. Am. J. Physiol. Lung Cell. Mol. Physiol. 276: L650-L658 [Abstract] [Full Text]
Stamme, C., Wright, J. R. (1999). Surfactant protein A enhances the binding and deacylation of E.�coli LPS by alveolar macrophages. Am. J. Physiol. Lung Cell. Mol. Physiol. 276: L540-L547 [Abstract] [Full Text]
Tino, M. J., Wright, J. R. (1999). Surfactant proteins A and D specifically stimulate directed actin-based responses in alveolar macrophages. Am. J. Physiol. Lung Cell. Mol. Physiol. 276: L164-L174 [Abstract] [Full Text]
Crouch, E. C. (1998). Collectins and Pulmonary Host Defense. Am. J. Respir. Cell Mol. Bio. 19: 177-201 [Abstract] [Full Text]
Mason, R. J., Greene, K., Voelker, D. R. (1998). Surfactant protein A and surfactant protein D in health and disease. Am. J. Physiol. Lung Cell. Mol. Physiol. 275: L1-L13 [Abstract] [Full Text]
Mishra, A., Weaver, T. E., Beck, D. C., Rothenberg, M. E. (2001). Interleukin-5-mediated Allergic Airway Inflammation Inhibits the Human Surfactant Protein C Promoter in Transgenic Mice. J. Biol. Chem. 276: 8453-8459 [Abstract] [Full Text]
Zhang, L., Ikegami, M., Crouch, E. C., Korfhagen, T. R., Whitsett, J. A. (2001). Activity of Pulmonary Surfactant Protein-D (SP-D) in Vivo Is Dependent on Oligomeric Structure. J. Biol. Chem. 276: 19214-19219 [Abstract] [Full Text]

J. Bacteriol.	J. Virol.	Eukaryot. Cell

Microbiol. Mol. Biol. Rev.	Clin. Vaccine Immunol.	All ASM Journals