Infect. Immun., Sep 1997, 3571-3576, Vol 65, No. 9
Copyright © 1997, American Society for Microbiology

Comparison of immune responses in patients infected with Vibrio cholerae O139 and O1

F Qadri, C Wenneras, MJ Albert, J Hossain, K Mannoor, YA Begum, G Mohi, MA Salam, RB Sack and AM Svennerholm
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka. fqadri@cholera.bangla.net

Vibrio cholerae O139 has recently emerged as the second etiologic agent of cholera in Asia. A study was carried out to evaluate the induction of specific immune responses to the organism in V. cholerae O139- infected patients. The immune responses to V. cholerae O139 Bengal were studied in patients by measuring antibody-secreting cells (ASC), as well as vibriocidal and antitoxic antibodies in the circulation. These responses were compared with those in patients with V. cholerae O1 disease. Strong immunoglobulin A (IgA) and IgM ASC responses were seen against the homologous lipopolysaccharide or serogroup of V. cholerae. The magnitude and isotype of the responses were similar in O139- and O1- infected patients. Vibriocidal antibody responses were seen against bacteria of the homologous but not heterologous serogroup, and these responses reflect the lack of cross-protection between the infections caused by the two serogroups. The two groups of patients showed comparable cholera toxin-specific ASC responses, with the IgG isotype dominating over the IgA isotype, as well as comparable antitoxic immune responses in plasma. These results suggest that despite having a polysaccharide capsule, V. cholerae O139 induces systemic and intestine- derived ASC responses in peripheral blood comparable to those seen in patients with V. cholerae O1 disease.

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